Isolation of microbial natural products.
(2012) In Methods in Molecular Biology 864. p.393-413- Abstract
- In principle, the isolation of secondary metabolites from microbes does not differ from their isolation from other organisms. The extraction procedure may of course be quite different, especially if it is carried out in an industrial scale, but when an extract containing the metabolites of interest is at hand, it is the same palette of adsorbents and chromatographic techniques that provide the major tools for the fractionation and eventual isolation of the pure compounds. Compared to plants, in which one is sure to find secondary metabolites of certain types, e.g., flavonoids, microbes can be expected to produce virtually anything and it is important to go about the fractionation procedure with an open mind. This chapter presents an... (More)
- In principle, the isolation of secondary metabolites from microbes does not differ from their isolation from other organisms. The extraction procedure may of course be quite different, especially if it is carried out in an industrial scale, but when an extract containing the metabolites of interest is at hand, it is the same palette of adsorbents and chromatographic techniques that provide the major tools for the fractionation and eventual isolation of the pure compounds. Compared to plants, in which one is sure to find secondary metabolites of certain types, e.g., flavonoids, microbes can be expected to produce virtually anything and it is important to go about the fractionation procedure with an open mind. This chapter presents an overview of preparation of extracts from microbial sources, and various methods and strategies involved in the isolation and characterization of microbial natural products. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2432491
- author
- Sterner, Olov LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Methods in Molecular Biology
- volume
- 864
- pages
- 393 - 413
- publisher
- Springer
- external identifiers
-
- pmid:22367905
- scopus:84859816386
- pmid:22367905
- ISSN
- 1940-6029
- DOI
- 10.1007/978-1-61779-624-1_15
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Organic chemistry (S/LTH) (011001240)
- id
- b81ae55d-9634-4ae8-80fc-e3e957728de5 (old id 2432491)
- date added to LUP
- 2016-04-01 10:36:18
- date last changed
- 2022-01-26 00:50:43
@article{b81ae55d-9634-4ae8-80fc-e3e957728de5,
abstract = {{In principle, the isolation of secondary metabolites from microbes does not differ from their isolation from other organisms. The extraction procedure may of course be quite different, especially if it is carried out in an industrial scale, but when an extract containing the metabolites of interest is at hand, it is the same palette of adsorbents and chromatographic techniques that provide the major tools for the fractionation and eventual isolation of the pure compounds. Compared to plants, in which one is sure to find secondary metabolites of certain types, e.g., flavonoids, microbes can be expected to produce virtually anything and it is important to go about the fractionation procedure with an open mind. This chapter presents an overview of preparation of extracts from microbial sources, and various methods and strategies involved in the isolation and characterization of microbial natural products.}},
author = {{Sterner, Olov}},
issn = {{1940-6029}},
language = {{eng}},
pages = {{393--413}},
publisher = {{Springer}},
series = {{Methods in Molecular Biology}},
title = {{Isolation of microbial natural products.}},
url = {{http://dx.doi.org/10.1007/978-1-61779-624-1_15}},
doi = {{10.1007/978-1-61779-624-1_15}},
volume = {{864}},
year = {{2012}},
}