Elevated Enterotoxin A Expression and Formation in Staphylococcus aureus and its Association with Prophage Induction.
(2012) In Applied and Environmental Microbiology 78(14). p.4942-4948- Abstract
- Staphylococcus aureus strains producing the bacteriophage-encoded staphylococcal enterotoxin A (SEA) were divided into two groups, high- and low-SEA-producing strains, based on the amount of SEA produced. After growth under favorable conditions in batch cultures, ten of the 21 strains tested produced more than 1,000 ng/ml SEA and nine strains produced less than 10 ng/ml SEA; two enterotoxigenic strains, MRSA252 and Newman, produced intermediate levels of SEA (around 450 ng/ml). The differences in the production of SEA were found to be associated with the expression level of sea and whether they hosted the versions sea(1) or sea(2). Furthermore, differences in the nucleotide sequence in the Siphoviridae phage region showed two clonal... (More)
- Staphylococcus aureus strains producing the bacteriophage-encoded staphylococcal enterotoxin A (SEA) were divided into two groups, high- and low-SEA-producing strains, based on the amount of SEA produced. After growth under favorable conditions in batch cultures, ten of the 21 strains tested produced more than 1,000 ng/ml SEA and nine strains produced less than 10 ng/ml SEA; two enterotoxigenic strains, MRSA252 and Newman, produced intermediate levels of SEA (around 450 ng/ml). The differences in the production of SEA were found to be associated with the expression level of sea and whether they hosted the versions sea(1) or sea(2). Furthermore, differences in the nucleotide sequence in the Siphoviridae phage region showed two clonal lineages of the high-SEA-producing strains. One of these lines was correlated with the capacity for a massive increase in SEA levels by prophage induction as demonstrated using mitomycin C (MC). This was also confirmed by the occurrence of additional sea expression presumed to be initiated by a latent phage promoter located upstream of the endogenous sea promoter. Remarkably, the SEA level was increased by up to ten fold in some strains due to prophage induction. The low-SEA-producing group and the high-SEA-producing subgroup lacking phage activated sea transcription showed no increase in SEA formation after the addition of MC. This study demonstrates that sea expression in enterotoxigenic strains is correlated with the clonal lineage of sea-encoded phages. The high-SEA-producing group, and in particular the prophage inducible sea(1) group, may be more relevant in staphylococcal food poisoning than the low-SEA-producing group, mainly harboring sea(2). (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2609363
- author
- Cao, Rong LU ; Zeaki, Nikoleta LU ; Wallin, Nina LU ; Skandamis, Panagiotis N ; Schelin, Jenny LU and Rådström, Peter LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Applied and Environmental Microbiology
- volume
- 78
- issue
- 14
- pages
- 4942 - 4948
- publisher
- American Society for Microbiology
- external identifiers
-
- wos:000306098600021
- pmid:22544256
- scopus:84863731315
- pmid:22544256
- ISSN
- 0099-2240
- DOI
- 10.1128/AEM.00803-12
- language
- English
- LU publication?
- yes
- id
- b2867b30-14ec-4e5b-88f6-39d329de4aab (old id 2609363)
- date added to LUP
- 2016-04-01 10:59:17
- date last changed
- 2022-04-28 03:30:57
@article{b2867b30-14ec-4e5b-88f6-39d329de4aab,
abstract = {{Staphylococcus aureus strains producing the bacteriophage-encoded staphylococcal enterotoxin A (SEA) were divided into two groups, high- and low-SEA-producing strains, based on the amount of SEA produced. After growth under favorable conditions in batch cultures, ten of the 21 strains tested produced more than 1,000 ng/ml SEA and nine strains produced less than 10 ng/ml SEA; two enterotoxigenic strains, MRSA252 and Newman, produced intermediate levels of SEA (around 450 ng/ml). The differences in the production of SEA were found to be associated with the expression level of sea and whether they hosted the versions sea(1) or sea(2). Furthermore, differences in the nucleotide sequence in the Siphoviridae phage region showed two clonal lineages of the high-SEA-producing strains. One of these lines was correlated with the capacity for a massive increase in SEA levels by prophage induction as demonstrated using mitomycin C (MC). This was also confirmed by the occurrence of additional sea expression presumed to be initiated by a latent phage promoter located upstream of the endogenous sea promoter. Remarkably, the SEA level was increased by up to ten fold in some strains due to prophage induction. The low-SEA-producing group and the high-SEA-producing subgroup lacking phage activated sea transcription showed no increase in SEA formation after the addition of MC. This study demonstrates that sea expression in enterotoxigenic strains is correlated with the clonal lineage of sea-encoded phages. The high-SEA-producing group, and in particular the prophage inducible sea(1) group, may be more relevant in staphylococcal food poisoning than the low-SEA-producing group, mainly harboring sea(2).}},
author = {{Cao, Rong and Zeaki, Nikoleta and Wallin, Nina and Skandamis, Panagiotis N and Schelin, Jenny and Rådström, Peter}},
issn = {{0099-2240}},
language = {{eng}},
number = {{14}},
pages = {{4942--4948}},
publisher = {{American Society for Microbiology}},
series = {{Applied and Environmental Microbiology}},
title = {{Elevated Enterotoxin A Expression and Formation in Staphylococcus aureus and its Association with Prophage Induction.}},
url = {{http://dx.doi.org/10.1128/AEM.00803-12}},
doi = {{10.1128/AEM.00803-12}},
volume = {{78}},
year = {{2012}},
}