Computational Design of Self-Assembling Protein Nanomaterials with Atomic Level Accuracy
(2012) In Science 336(6085). p.1171-1174- Abstract
- We describe a general computational method for designing proteins that self-assemble to a desired symmetric architecture. Protein building blocks are docked together symmetrically to identify complementary packing arrangements, and low-energy protein-protein interfaces are then designed between the building blocks in order to drive self-assembly. We used trimeric protein building blocks to design a 24-subunit, 13-nm diameter complex with octahedral symmetry and a 12-subunit, 11-nm diameter complex with tetrahedral symmetry. The designed proteins assembled to the desired oligomeric states in solution, and the crystal structures of the complexes revealed that the resulting materials closely match the design models. The method can be used to... (More)
- We describe a general computational method for designing proteins that self-assemble to a desired symmetric architecture. Protein building blocks are docked together symmetrically to identify complementary packing arrangements, and low-energy protein-protein interfaces are then designed between the building blocks in order to drive self-assembly. We used trimeric protein building blocks to design a 24-subunit, 13-nm diameter complex with octahedral symmetry and a 12-subunit, 11-nm diameter complex with tetrahedral symmetry. The designed proteins assembled to the desired oligomeric states in solution, and the crystal structures of the complexes revealed that the resulting materials closely match the design models. The method can be used to design a wide variety of self-assembling protein nanomaterials. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2799758
- author
- King, Neil P. ; Sheffler, William ; Sawaya, Michael R. ; Vollmar, Breanna S. ; Sumida, John P. ; André, Ingemar LU ; Gonen, Tamir ; Yeates, Todd O. and Baker, David
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Science
- volume
- 336
- issue
- 6085
- pages
- 1171 - 1174
- publisher
- American Association for the Advancement of Science (AAAS)
- external identifiers
-
- wos:000304647900053
- scopus:84861676223
- pmid:22654060
- ISSN
- 1095-9203
- DOI
- 10.1126/science.1219364
- language
- English
- LU publication?
- yes
- id
- 34bfdfba-8f6c-4480-93ef-d5f4bf31b625 (old id 2799758)
- date added to LUP
- 2016-04-01 13:32:51
- date last changed
- 2022-04-06 05:32:12
@article{34bfdfba-8f6c-4480-93ef-d5f4bf31b625, abstract = {{We describe a general computational method for designing proteins that self-assemble to a desired symmetric architecture. Protein building blocks are docked together symmetrically to identify complementary packing arrangements, and low-energy protein-protein interfaces are then designed between the building blocks in order to drive self-assembly. We used trimeric protein building blocks to design a 24-subunit, 13-nm diameter complex with octahedral symmetry and a 12-subunit, 11-nm diameter complex with tetrahedral symmetry. The designed proteins assembled to the desired oligomeric states in solution, and the crystal structures of the complexes revealed that the resulting materials closely match the design models. The method can be used to design a wide variety of self-assembling protein nanomaterials.}}, author = {{King, Neil P. and Sheffler, William and Sawaya, Michael R. and Vollmar, Breanna S. and Sumida, John P. and André, Ingemar and Gonen, Tamir and Yeates, Todd O. and Baker, David}}, issn = {{1095-9203}}, language = {{eng}}, number = {{6085}}, pages = {{1171--1174}}, publisher = {{American Association for the Advancement of Science (AAAS)}}, series = {{Science}}, title = {{Computational Design of Self-Assembling Protein Nanomaterials with Atomic Level Accuracy}}, url = {{http://dx.doi.org/10.1126/science.1219364}}, doi = {{10.1126/science.1219364}}, volume = {{336}}, year = {{2012}}, }