Arabidopsis STO/BBX24 negatively regulates UV-B signaling by interacting with COP1 and repressing HY5 transcriptional activity
Jiang, Lei ; Wang, Yan ; Li, Qian-Feng ; Björn, Lars Olof LU
; He, Jun-Xian
and Li, Shao-Shan
(2012)
In Cell Research
22.
p.1046-1057
- Abstract
- UV-B (280-315 nm) is an integral part of solar radiation and can act either as a stress inducer or as a developmental signal. In recent years, increasing attention has been paid to the low-fluence UV-B-induced photomorphogenic response and several key players in this response have been identified, which include UVR8 (a UV-B-specific photoreceptor), COP1 (a WD40-repeat-containing RING finger protein), HY5 (a basic zipper transcription factor), and RUP1/2 (two UVR8-interacting proteins). Here we report that Arabidopsis SALT TOLERANCE (STO/BBX24), a known regulator for light signaling in plants, defines a new signaling component in UV-B-mediated photomorphogenesis. The bbx24 mutant is hypersensitive to UV-B radiation and becomes extremely... (More)
- UV-B (280-315 nm) is an integral part of solar radiation and can act either as a stress inducer or as a developmental signal. In recent years, increasing attention has been paid to the low-fluence UV-B-induced photomorphogenic response and several key players in this response have been identified, which include UVR8 (a UV-B-specific photoreceptor), COP1 (a WD40-repeat-containing RING finger protein), HY5 (a basic zipper transcription factor), and RUP1/2 (two UVR8-interacting proteins). Here we report that Arabidopsis SALT TOLERANCE (STO/BBX24), a known regulator for light signaling in plants, defines a new signaling component in UV-B-mediated photomorphogenesis. The bbx24 mutant is hypersensitive to UV-B radiation and becomes extremely dwarfed under UV-B treatment. By contrast, BBX24 overexpression transgenic lines respond much more weakly to UV-B than the bbx24 and wild-type plants.
BBX24 expression is UV-B-inducible and its accumulation under UV-B requires COP1. Co-immunoprecipitation
experiments indicate that BBX24 interacts with COP1 in planta upon UV-B illumination. Moreover, BBX24 interacts
with HY5 and acts antagonistically with HY5 in UV-B-induced inhibition of hypocotyl elongation. Furthermore,
BBX24 attenuates UV-B-induced HY5 accumulation and suppresses its transcription-activation activity. Taken together, our results reveal a previously uncharacterized function of the light-regulated BBX24 in UV-B responses and demonstrate that BBX24 functions as a negative regulator of photomorphogenic UV-B responses by interacting with both COP1 and HY5. The UV-B-inducible expression pattern and its suppression of HY5 activity suggest that BBX24 could be a new component of the feedback regulatory module of UV-B signaling in plants. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2860496
- author
- Jiang, Lei
; Wang, Yan
; Li, Qian-Feng
; Björn, Lars Olof
LU
; He, Jun-Xian
and Li, Shao-Shan
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Arabidopsis thaliana, HY5, COP1, STO/BBX24, photomorphogenesis, UV-B
- in
- Cell Research
- volume
- 22
- pages
- 1046 - 1057
- publisher
- Science Press
- external identifiers
-
- wos:000304817300011
- scopus:84862832310
- pmid:22410790
- ISSN
- 1748-7838
- DOI
- 10.1038/cr.2012.34
- project
- Photobiology
- language
- English
- LU publication?
- yes
- id
- 04bc5b46-b7b5-45c0-aa6d-cb29821f6308 (old id 2860496)
- date added to LUP
- 2016-04-01 09:54:32
- date last changed
- 2022-03-04 06:09:16
@article{04bc5b46-b7b5-45c0-aa6d-cb29821f6308,
abstract = {{UV-B (280-315 nm) is an integral part of solar radiation and can act either as a stress inducer or as a developmental signal. In recent years, increasing attention has been paid to the low-fluence UV-B-induced photomorphogenic response and several key players in this response have been identified, which include UVR8 (a UV-B-specific photoreceptor), COP1 (a WD40-repeat-containing RING finger protein), HY5 (a basic zipper transcription factor), and RUP1/2 (two UVR8-interacting proteins). Here we report that Arabidopsis SALT TOLERANCE (STO/BBX24), a known regulator for light signaling in plants, defines a new signaling component in UV-B-mediated photomorphogenesis. The bbx24 mutant is hypersensitive to UV-B radiation and becomes extremely dwarfed under UV-B treatment. By contrast, BBX24 overexpression transgenic lines respond much more weakly to UV-B than the bbx24 and wild-type plants.<br/><br>
BBX24 expression is UV-B-inducible and its accumulation under UV-B requires COP1. Co-immunoprecipitation<br/><br>
experiments indicate that BBX24 interacts with COP1 in planta upon UV-B illumination. Moreover, BBX24 interacts<br/><br>
with HY5 and acts antagonistically with HY5 in UV-B-induced inhibition of hypocotyl elongation. Furthermore,<br/><br>
BBX24 attenuates UV-B-induced HY5 accumulation and suppresses its transcription-activation activity. Taken together, our results reveal a previously uncharacterized function of the light-regulated BBX24 in UV-B responses and demonstrate that BBX24 functions as a negative regulator of photomorphogenic UV-B responses by interacting with both COP1 and HY5. The UV-B-inducible expression pattern and its suppression of HY5 activity suggest that BBX24 could be a new component of the feedback regulatory module of UV-B signaling in plants.}},
author = {{Jiang, Lei and Wang, Yan and Li, Qian-Feng and Björn, Lars Olof and He, Jun-Xian and Li, Shao-Shan}},
issn = {{1748-7838}},
keywords = {{Arabidopsis thaliana; HY5; COP1; STO/BBX24; photomorphogenesis; UV-B}},
language = {{eng}},
pages = {{1046--1057}},
publisher = {{Science Press}},
series = {{Cell Research}},
title = {{Arabidopsis STO/BBX24 negatively regulates UV-B signaling by interacting with COP1 and repressing HY5 transcriptional activity}},
url = {{http://dx.doi.org/10.1038/cr.2012.34}},
doi = {{10.1038/cr.2012.34}},
volume = {{22}},
year = {{2012}},
}