CC chemokine ligand 20 partially controls adhesion of naive B cells to activated endothelial cells under shear stress
(2003) In Blood 102(8). p.2724-2727- Abstract
- Chemokines are thought to control lymphocyte recruitment to the inflamed endothelium. To dissect chemokine-mediated adhesion, binding of ex vivo isolated splenocytes to tumor necrosis factor (TNF)-activated endothelial cells was analyzed under shear stress. We observed specific adhesion of naive follicular B cells, which could be blocked by pertussis toxin. This indicated a G protein-mediated binding and pointed at a contribution of chemokine receptors to B-cell adhesion. Analysis of chemokines expressed by TNF-activated endothelial cells showed that CC chemokine ligand 2 (CCL2), CCL17, and CCL20 were up-regulated. Only on follicular B cells was the cognate receptor for CCL20, CC chemokine receptor 6 (CCR6), expressed strongly, and a... (More)
- Chemokines are thought to control lymphocyte recruitment to the inflamed endothelium. To dissect chemokine-mediated adhesion, binding of ex vivo isolated splenocytes to tumor necrosis factor (TNF)-activated endothelial cells was analyzed under shear stress. We observed specific adhesion of naive follicular B cells, which could be blocked by pertussis toxin. This indicated a G protein-mediated binding and pointed at a contribution of chemokine receptors to B-cell adhesion. Analysis of chemokines expressed by TNF-activated endothelial cells showed that CC chemokine ligand 2 (CCL2), CCL17, and CCL20 were up-regulated. Only on follicular B cells was the cognate receptor for CCL20, CC chemokine receptor 6 (CCR6), expressed strongly, and a functional transmigration assay with CCR6-negative B cells demonstrated conclusively the sole signaling of CCL20 through CCR6. Desensitization of CCR6 on naive B cells with CCL20 resulted in receptor down-regulation and reduced B-cell adhesion. We conclude that CCL20 plays a vital role in B-cell adhesion to the inflamed endothelium. (C) 2003 by The American Society of Hematology. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/298496
- author
- Meissner, A ; Zilles, Olaf LU ; Varona, R ; Jozefowski, K ; Ritter, U ; Marquez, G ; Hallmann, Rupert LU and Korner, H
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Blood
- volume
- 102
- issue
- 8
- pages
- 2724 - 2727
- publisher
- American Society of Hematology
- external identifiers
-
- pmid:12816871
- wos:000185877300012
- scopus:0141993068
- ISSN
- 1528-0020
- DOI
- 10.1182/blood-2003-01-0007
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pathology, (Lund) (013030000)
- id
- 9447b2ea-4931-42d3-a195-8324a6d1f6ba (old id 298496)
- date added to LUP
- 2016-04-01 11:56:29
- date last changed
- 2022-03-28 17:55:46
@article{9447b2ea-4931-42d3-a195-8324a6d1f6ba, abstract = {{Chemokines are thought to control lymphocyte recruitment to the inflamed endothelium. To dissect chemokine-mediated adhesion, binding of ex vivo isolated splenocytes to tumor necrosis factor (TNF)-activated endothelial cells was analyzed under shear stress. We observed specific adhesion of naive follicular B cells, which could be blocked by pertussis toxin. This indicated a G protein-mediated binding and pointed at a contribution of chemokine receptors to B-cell adhesion. Analysis of chemokines expressed by TNF-activated endothelial cells showed that CC chemokine ligand 2 (CCL2), CCL17, and CCL20 were up-regulated. Only on follicular B cells was the cognate receptor for CCL20, CC chemokine receptor 6 (CCR6), expressed strongly, and a functional transmigration assay with CCR6-negative B cells demonstrated conclusively the sole signaling of CCL20 through CCR6. Desensitization of CCR6 on naive B cells with CCL20 resulted in receptor down-regulation and reduced B-cell adhesion. We conclude that CCL20 plays a vital role in B-cell adhesion to the inflamed endothelium. (C) 2003 by The American Society of Hematology.}}, author = {{Meissner, A and Zilles, Olaf and Varona, R and Jozefowski, K and Ritter, U and Marquez, G and Hallmann, Rupert and Korner, H}}, issn = {{1528-0020}}, language = {{eng}}, number = {{8}}, pages = {{2724--2727}}, publisher = {{American Society of Hematology}}, series = {{Blood}}, title = {{CC chemokine ligand 20 partially controls adhesion of naive B cells to activated endothelial cells under shear stress}}, url = {{http://dx.doi.org/10.1182/blood-2003-01-0007}}, doi = {{10.1182/blood-2003-01-0007}}, volume = {{102}}, year = {{2003}}, }