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Optimization of steam pretreatment of corn stover to enhance enzymatic digestibility

Varga, Arthur LU ; Reczey, K and Zacchi, Guido LU (2004) In Applied Biochemistry and Biotechnology 114(1-3). p.509-523
Abstract
Among the available agricultural byproducts, corn stover, with its yearly production of 10 million t (dry basis), is the most abundant promising raw material for fuel ethanol production in Hungary. In the United States, more than 216 million t of corn stover is produced annually, of which a portion also could possibly be collected for conversion to ethanol. However, a network of lignin and hemicellulose protects cellulose, which is the major source of fermentable sugars in corn stover (approx 40% of the dry matter [DM]). Steam pretreatment removes the major part of the hemicellulose from the solid material and makes the cellulose more susceptible to enzymatic digestion. We studied 12 different combinations of reaction temperature, time,... (More)
Among the available agricultural byproducts, corn stover, with its yearly production of 10 million t (dry basis), is the most abundant promising raw material for fuel ethanol production in Hungary. In the United States, more than 216 million t of corn stover is produced annually, of which a portion also could possibly be collected for conversion to ethanol. However, a network of lignin and hemicellulose protects cellulose, which is the major source of fermentable sugars in corn stover (approx 40% of the dry matter [DM]). Steam pretreatment removes the major part of the hemicellulose from the solid material and makes the cellulose more susceptible to enzymatic digestion. We studied 12 different combinations of reaction temperature, time, and pH during steam pretreatment. The best conditions (200degreesC, 5 min, 2% H2SO4) increased the enzymatic conversion (from cellulose to glucose) of corn stover more then four times, compared to untreated material. However, steam pretreatment at 190degreesC for 5 min with 2% sulfuric acid resulted in the highest overall yield of sugars, 56.1 g from 100 g of untreated material (DM), corresponding to 73% of the theoretical. The liquor following steam explosion was fermented using Saccharomyces cerevisiae to investigate the inhibitory effect of the pretreatment. The achieved ethanol yield was slightly higher than that obtained with a reference sugar solution. This demonstrates that baker's yeast could adapt to the pretreated liquor and ferment the glucose to ethanol efficiently. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied Biochemistry and Biotechnology
volume
114
issue
1-3
pages
509 - 523
publisher
Humana Press
external identifiers
  • wos:000221186200044
  • scopus:11244262290
ISSN
1559-0291
DOI
10.1385/ABAB:114:1-3:509
language
English
LU publication?
yes
id
2b363302-2378-4017-80c2-eb38fbd9d6b8 (old id 138856)
date added to LUP
2016-04-01 12:17:53
date last changed
2023-11-11 20:06:59
@article{2b363302-2378-4017-80c2-eb38fbd9d6b8,
  abstract     = {{Among the available agricultural byproducts, corn stover, with its yearly production of 10 million t (dry basis), is the most abundant promising raw material for fuel ethanol production in Hungary. In the United States, more than 216 million t of corn stover is produced annually, of which a portion also could possibly be collected for conversion to ethanol. However, a network of lignin and hemicellulose protects cellulose, which is the major source of fermentable sugars in corn stover (approx 40% of the dry matter [DM]). Steam pretreatment removes the major part of the hemicellulose from the solid material and makes the cellulose more susceptible to enzymatic digestion. We studied 12 different combinations of reaction temperature, time, and pH during steam pretreatment. The best conditions (200degreesC, 5 min, 2% H2SO4) increased the enzymatic conversion (from cellulose to glucose) of corn stover more then four times, compared to untreated material. However, steam pretreatment at 190degreesC for 5 min with 2% sulfuric acid resulted in the highest overall yield of sugars, 56.1 g from 100 g of untreated material (DM), corresponding to 73% of the theoretical. The liquor following steam explosion was fermented using Saccharomyces cerevisiae to investigate the inhibitory effect of the pretreatment. The achieved ethanol yield was slightly higher than that obtained with a reference sugar solution. This demonstrates that baker's yeast could adapt to the pretreated liquor and ferment the glucose to ethanol efficiently.}},
  author       = {{Varga, Arthur and Reczey, K and Zacchi, Guido}},
  issn         = {{1559-0291}},
  language     = {{eng}},
  number       = {{1-3}},
  pages        = {{509--523}},
  publisher    = {{Humana Press}},
  series       = {{Applied Biochemistry and Biotechnology}},
  title        = {{Optimization of steam pretreatment of corn stover to enhance enzymatic digestibility}},
  url          = {{http://dx.doi.org/10.1385/ABAB:114:1-3:509}},
  doi          = {{10.1385/ABAB:114:1-3:509}},
  volume       = {{114}},
  year         = {{2004}},
}