Characterization and criteria of embryonic stem and induced pluripotent stem cells for a dopamine replacement therapy
Cooper, Oliver ; Parmar, Malin LU
and Isacson, Ole
(2012)
In Progress in Brain Research
200.
p.76-265
- Abstract
Human pluripotent stem cells provide new choices for sources of A9-type dopaminergic (DA) neurons in clinical trials of neural transplantation for patients with Parkinson's disease (PD). For example, "self" and HLA-matched A9 DA neurons may improve the patient-to-patient variability observed in previous clinical trials using fetal DA neurons and obviate the need for long-term immunosuppression in the patient. Normal chromosomal structure and minimal somatic mutations in pluripotent stem cells are necessary criteria for assuring the safe and reproducible transplantation of differentiated DA neurons into patients with PD in clinical trials. However, with these new choices of cell source, the application of pluripotency assays as criteria... (More)
Human pluripotent stem cells provide new choices for sources of A9-type dopaminergic (DA) neurons in clinical trials of neural transplantation for patients with Parkinson's disease (PD). For example, "self" and HLA-matched A9 DA neurons may improve the patient-to-patient variability observed in previous clinical trials using fetal DA neurons and obviate the need for long-term immunosuppression in the patient. Normal chromosomal structure and minimal somatic mutations in pluripotent stem cells are necessary criteria for assuring the safe and reproducible transplantation of differentiated DA neurons into patients with PD in clinical trials. However, with these new choices of cell source, the application of pluripotency assays as criteria to ensure pluripotent stem cell quality becomes less relevant. New more relevant standards of quality control, assurance, and function are required. We suggest that quality assurance measures for pluripotent stem cells need to focus upon readouts for authentic midbrain DA neurons, their integration and growth using in vivo assays, and their long-term functional stability.
(Less)
- author
- Cooper, Oliver
; Parmar, Malin
LU
and Isacson, Ole
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animals, Cell Differentiation, Dopaminergic Neurons, Embryonic Stem Cells, Humans, Induced Pluripotent Stem Cells, Mesencephalon, Parkinson Disease, Stem Cell Transplantation, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Review
- in
- Progress in Brain Research
- volume
- 200
- pages
- 12 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:23195423
- scopus:84870275738
- ISSN
- 1875-7855
- DOI
- 10.1016/B978-0-444-59575-1.00012-0
- language
- English
- LU publication?
- yes
- id
- 2d86bc77-a588-4f7c-b973-7624d09edf11
- date added to LUP
- 2016-11-23 14:28:20
- date last changed
- 2024-03-07 16:41:57
@article{2d86bc77-a588-4f7c-b973-7624d09edf11,
abstract = {{<p>Human pluripotent stem cells provide new choices for sources of A9-type dopaminergic (DA) neurons in clinical trials of neural transplantation for patients with Parkinson's disease (PD). For example, "self" and HLA-matched A9 DA neurons may improve the patient-to-patient variability observed in previous clinical trials using fetal DA neurons and obviate the need for long-term immunosuppression in the patient. Normal chromosomal structure and minimal somatic mutations in pluripotent stem cells are necessary criteria for assuring the safe and reproducible transplantation of differentiated DA neurons into patients with PD in clinical trials. However, with these new choices of cell source, the application of pluripotency assays as criteria to ensure pluripotent stem cell quality becomes less relevant. New more relevant standards of quality control, assurance, and function are required. We suggest that quality assurance measures for pluripotent stem cells need to focus upon readouts for authentic midbrain DA neurons, their integration and growth using in vivo assays, and their long-term functional stability.</p>}},
author = {{Cooper, Oliver and Parmar, Malin and Isacson, Ole}},
issn = {{1875-7855}},
keywords = {{Animals; Cell Differentiation; Dopaminergic Neurons; Embryonic Stem Cells; Humans; Induced Pluripotent Stem Cells; Mesencephalon; Parkinson Disease; Stem Cell Transplantation; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review}},
language = {{eng}},
pages = {{76--265}},
publisher = {{Elsevier}},
series = {{Progress in Brain Research}},
title = {{Characterization and criteria of embryonic stem and induced pluripotent stem cells for a dopamine replacement therapy}},
url = {{http://dx.doi.org/10.1016/B978-0-444-59575-1.00012-0}},
doi = {{10.1016/B978-0-444-59575-1.00012-0}},
volume = {{200}},
year = {{2012}},
}