Chitosan does not inhibit enzymatic action of human pancreatic lipase in Langmuir monolayers of 1,2-didecanoyl-glycerol (DDG)
(2014) In Colloids and Surfaces B: Biointerfaces 123. p.870-877- Abstract
In this study, we tested the hypothesis according to which chitosan reduces lipid digestion by blocking the access of lipases to ingested fat. Because lipase action takes place mostly at interfaces, we produced Langmuir films of 1,2-didecanoyl-glycerol (DDG), which is the substrate for human pancreatic lipase (HPL). The experimental assays were carried out in acidic medium, at pH 3.0, to ensure that chitosan is completely soluble. Chitosan was found to affect strongly the surface activity of HPL that forms a Gibbs monolayer at the air/water interface, but did not inhibit the enzymatic action of HPL toward the DDG monolayer. The latter was observed using two surface-specific spectroscopic techniques, namely polarization-modulated... (More)
In this study, we tested the hypothesis according to which chitosan reduces lipid digestion by blocking the access of lipases to ingested fat. Because lipase action takes place mostly at interfaces, we produced Langmuir films of 1,2-didecanoyl-glycerol (DDG), which is the substrate for human pancreatic lipase (HPL). The experimental assays were carried out in acidic medium, at pH 3.0, to ensure that chitosan is completely soluble. Chitosan was found to affect strongly the surface activity of HPL that forms a Gibbs monolayer at the air/water interface, but did not inhibit the enzymatic action of HPL toward the DDG monolayer. The latter was observed using two surface-specific spectroscopic techniques, namely polarization-modulated infrared reflection-absorption and sum-frequency generation (SFG). The extension of DDG hydrolysis calculated using SFG spectroscopy was 33% in the absence of chitosan, and ranged from 29 to 50% in the presence of chitosan at concentrations of 0.20gL-1 and 0.30gL-1, respectively. Therefore, fat "protection" by chitosan is unlikely to be an important factor in fat reduction.
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- author
- Souza, Adriano L. ; Pavinatto, Felippe J. ; Caseli, Luciano ; Volpati, Diogo LU ; Miranda, Paulo B. and Oliveira, Osvaldo N.
- publishing date
- 2014-11-01
- type
- Contribution to journal
- publication status
- published
- keywords
- Chitosan, Fat reduction, Human pancreatic lipase, Langmuir monolayers, Sum-frequency generation spectroscopy
- in
- Colloids and Surfaces B: Biointerfaces
- volume
- 123
- pages
- 8 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:84915823197
- ISSN
- 0927-7765
- DOI
- 10.1016/j.colsurfb.2014.10.040
- language
- English
- LU publication?
- no
- id
- 2d8de800-7ec3-491d-a094-511af53faf10
- date added to LUP
- 2019-05-17 14:33:34
- date last changed
- 2022-02-15 19:18:53
@article{2d8de800-7ec3-491d-a094-511af53faf10,
abstract = {{<p>In this study, we tested the hypothesis according to which chitosan reduces lipid digestion by blocking the access of lipases to ingested fat. Because lipase action takes place mostly at interfaces, we produced Langmuir films of 1,2-didecanoyl-glycerol (DDG), which is the substrate for human pancreatic lipase (HPL). The experimental assays were carried out in acidic medium, at pH 3.0, to ensure that chitosan is completely soluble. Chitosan was found to affect strongly the surface activity of HPL that forms a Gibbs monolayer at the air/water interface, but did not inhibit the enzymatic action of HPL toward the DDG monolayer. The latter was observed using two surface-specific spectroscopic techniques, namely polarization-modulated infrared reflection-absorption and sum-frequency generation (SFG). The extension of DDG hydrolysis calculated using SFG spectroscopy was 33% in the absence of chitosan, and ranged from 29 to 50% in the presence of chitosan at concentrations of 0.20gL<sup>-1</sup> and 0.30gL<sup>-1</sup>, respectively. Therefore, fat "protection" by chitosan is unlikely to be an important factor in fat reduction.</p>}},
author = {{Souza, Adriano L. and Pavinatto, Felippe J. and Caseli, Luciano and Volpati, Diogo and Miranda, Paulo B. and Oliveira, Osvaldo N.}},
issn = {{0927-7765}},
keywords = {{Chitosan; Fat reduction; Human pancreatic lipase; Langmuir monolayers; Sum-frequency generation spectroscopy}},
language = {{eng}},
month = {{11}},
pages = {{870--877}},
publisher = {{Elsevier}},
series = {{Colloids and Surfaces B: Biointerfaces}},
title = {{Chitosan does not inhibit enzymatic action of human pancreatic lipase in Langmuir monolayers of 1,2-didecanoyl-glycerol (DDG)}},
url = {{http://dx.doi.org/10.1016/j.colsurfb.2014.10.040}},
doi = {{10.1016/j.colsurfb.2014.10.040}},
volume = {{123}},
year = {{2014}},
}