Molecular identification of bloodmeals and species composition in Culicoides biting midges.
(2012) In Medical and Veterinary Entomology- Abstract
- Investigations of host preferences in haematophagous insects, including Culicoides biting midges (Diptera: Ceratopogonidae), are critical in order to assess transmission routes of vector-borne diseases. In this study, we collected and morphologically identified 164 blood-engorged Culicoides females caught in both light traps and permanent 12-m high suction traps during 2008-2010 in Sweden. Molecular analysis of the mitochondrial cytochrome c oxidase subunit I (COI) gene in the biting midges was performed to verify species classification, discern phylogenetic relationships and uncover possible cryptic species. Bloodmeal analysis using universal vertebrate cytochrome b primers revealed a clear distinction in host selection between... (More)
- Investigations of host preferences in haematophagous insects, including Culicoides biting midges (Diptera: Ceratopogonidae), are critical in order to assess transmission routes of vector-borne diseases. In this study, we collected and morphologically identified 164 blood-engorged Culicoides females caught in both light traps and permanent 12-m high suction traps during 2008-2010 in Sweden. Molecular analysis of the mitochondrial cytochrome c oxidase subunit I (COI) gene in the biting midges was performed to verify species classification, discern phylogenetic relationships and uncover possible cryptic species. Bloodmeal analysis using universal vertebrate cytochrome b primers revealed a clear distinction in host selection between mammalophilic and ornithophilic Culicoides species. Host sequences found matches in horse (n = 59), sheep (n = 39), cattle (n = 26), Eurasian elk (n = 1) and 10 different bird species (n = 18). We identified 15 Culicoides species previously recorded in Scandinavia and four additional species haplotypes that were distinctly different from the described species. All ornithophilic individuals (n = 23) were caught exclusively in the suction traps, as were, interestingly, almost all mammalophilic species (n = 41), indicating that many biting midge species may be able to cover long distances after completing a bloodmeal. These results add new information on the composition of Culicoides species and their host preferences and their potential long-distance dispersal while blood-engorged. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3047607
- author
- Videvall, Elin LU ; Bensch, Staffan LU ; Ander, M ; Chirico, J ; Sigvald, R and Ignell, R LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Medical and Veterinary Entomology
- publisher
- The Royal Entomological Society
- external identifiers
-
- wos:000315033000012
- pmid:22882691
- scopus:84873986490
- ISSN
- 0269-283X
- DOI
- 10.1111/j.1365-2915.2012.01038.x
- language
- English
- LU publication?
- yes
- id
- a18aff34-13a9-4129-ae18-8ce185fa7285 (old id 3047607)
- date added to LUP
- 2016-04-04 08:55:13
- date last changed
- 2022-04-23 18:20:42
@article{a18aff34-13a9-4129-ae18-8ce185fa7285,
abstract = {{Investigations of host preferences in haematophagous insects, including Culicoides biting midges (Diptera: Ceratopogonidae), are critical in order to assess transmission routes of vector-borne diseases. In this study, we collected and morphologically identified 164 blood-engorged Culicoides females caught in both light traps and permanent 12-m high suction traps during 2008-2010 in Sweden. Molecular analysis of the mitochondrial cytochrome c oxidase subunit I (COI) gene in the biting midges was performed to verify species classification, discern phylogenetic relationships and uncover possible cryptic species. Bloodmeal analysis using universal vertebrate cytochrome b primers revealed a clear distinction in host selection between mammalophilic and ornithophilic Culicoides species. Host sequences found matches in horse (n = 59), sheep (n = 39), cattle (n = 26), Eurasian elk (n = 1) and 10 different bird species (n = 18). We identified 15 Culicoides species previously recorded in Scandinavia and four additional species haplotypes that were distinctly different from the described species. All ornithophilic individuals (n = 23) were caught exclusively in the suction traps, as were, interestingly, almost all mammalophilic species (n = 41), indicating that many biting midge species may be able to cover long distances after completing a bloodmeal. These results add new information on the composition of Culicoides species and their host preferences and their potential long-distance dispersal while blood-engorged.}},
author = {{Videvall, Elin and Bensch, Staffan and Ander, M and Chirico, J and Sigvald, R and Ignell, R}},
issn = {{0269-283X}},
language = {{eng}},
publisher = {{The Royal Entomological Society}},
series = {{Medical and Veterinary Entomology}},
title = {{Molecular identification of bloodmeals and species composition in Culicoides biting midges.}},
url = {{http://dx.doi.org/10.1111/j.1365-2915.2012.01038.x}},
doi = {{10.1111/j.1365-2915.2012.01038.x}},
year = {{2012}},
}