The breast cancer-associated gene Di12 has oncogenic activity
(2003) In Anticancer research 23(3A). p.2027-2033- Abstract
- The breast cancer-associated gene Di12 encodes a novel protein, which was found overexpressed in invasive ductal carcinomas of the breast. In experiments designed to assess the role of the Di12 gene in oncogenesis, the overexpression of 339 N-terminal amino acids of this gene in NIH3T3 cells resulted in cellular transformation and in vivo tumorigenesis. NIH3T3-Di12 tumor cell growth was partly reversible upon Di12 antisense treatment. In addition, transfortnation of the ER + human breast cancer cell line MCF- 7 resulted in hormone independent growth of these tumors in nude mice. Di12 expression in NIH3T3 and MCF-7 tumor cells was confirmed by RT-PCR and mabDi12 immunostaining. Immunohistochemistry using mabDi12 on an arrayed collection of... (More)
- The breast cancer-associated gene Di12 encodes a novel protein, which was found overexpressed in invasive ductal carcinomas of the breast. In experiments designed to assess the role of the Di12 gene in oncogenesis, the overexpression of 339 N-terminal amino acids of this gene in NIH3T3 cells resulted in cellular transformation and in vivo tumorigenesis. NIH3T3-Di12 tumor cell growth was partly reversible upon Di12 antisense treatment. In addition, transfortnation of the ER + human breast cancer cell line MCF- 7 resulted in hormone independent growth of these tumors in nude mice. Di12 expression in NIH3T3 and MCF-7 tumor cells was confirmed by RT-PCR and mabDi12 immunostaining. Immunohistochemistry using mabDi12 on an arrayed collection of 106 invasive breast tumors further underlined the expression of the gene in over 75% of advanced stage breast cancers. Our data indicate that Di12 expression is oncogenic in in vitro transformation and in vivo tumorigenic assays. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/305794
- author
- Burger, AM ; Grugel, R ; Landberg, Göran LU ; Kitching, R and Seth, A
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- nude mice, mabDi12, tumor formation, Di12, clonogenic assay, gene, expression
- in
- Anticancer research
- volume
- 23
- issue
- 3A
- pages
- 2027 - 2033
- publisher
- International Institute of Cancer Research
- external identifiers
-
- wos:000184359100006
- scopus:0042306312
- ISSN
- 1791-7530
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pathology, (Lund) (013030000), Pathology (Malmö) (013031000)
- id
- 08d6cf6c-ac6c-46e6-b8d1-3c2c83b61e0f (old id 305794)
- date added to LUP
- 2016-04-01 11:50:24
- date last changed
- 2022-02-18 06:08:27
@article{08d6cf6c-ac6c-46e6-b8d1-3c2c83b61e0f, abstract = {{The breast cancer-associated gene Di12 encodes a novel protein, which was found overexpressed in invasive ductal carcinomas of the breast. In experiments designed to assess the role of the Di12 gene in oncogenesis, the overexpression of 339 N-terminal amino acids of this gene in NIH3T3 cells resulted in cellular transformation and in vivo tumorigenesis. NIH3T3-Di12 tumor cell growth was partly reversible upon Di12 antisense treatment. In addition, transfortnation of the ER + human breast cancer cell line MCF- 7 resulted in hormone independent growth of these tumors in nude mice. Di12 expression in NIH3T3 and MCF-7 tumor cells was confirmed by RT-PCR and mabDi12 immunostaining. Immunohistochemistry using mabDi12 on an arrayed collection of 106 invasive breast tumors further underlined the expression of the gene in over 75% of advanced stage breast cancers. Our data indicate that Di12 expression is oncogenic in in vitro transformation and in vivo tumorigenic assays.}}, author = {{Burger, AM and Grugel, R and Landberg, Göran and Kitching, R and Seth, A}}, issn = {{1791-7530}}, keywords = {{nude mice; mabDi12; tumor formation; Di12; clonogenic assay; gene; expression}}, language = {{eng}}, number = {{3A}}, pages = {{2027--2033}}, publisher = {{International Institute of Cancer Research}}, series = {{Anticancer research}}, title = {{The breast cancer-associated gene Di12 has oncogenic activity}}, volume = {{23}}, year = {{2003}}, }