Epithelial expression of matrix metalloproteinase-26 is elevated at mid-cycle in the human endometrium
(2003) In Molecular Human Reproduction 9(5). p.271-277- Abstract
- The human endometrium is a dynamic tissue, which undergoes extensive tissue remodelling during the menstrual cycle. Due to their involvement in such processes, several well-characterized matrix metalloproteinases (MMP) have previously been studied in the endometrium. MMP-26 is a newly described matrilysin. We studied MMP-26 mRNA in 39 normal endometrial samples obtained across the menstrual cycle. Tissue distribution and cycle variation was examined using in-situ hybridization, Northern blot analyis and real time PCR. The probes for Northern blot analysis and real time PCR recognized non-overlapping sequences. MMP-26 was localized exclusively in epithelial cells of both glands and the luminal surface. Expression increased during the... (More)
- The human endometrium is a dynamic tissue, which undergoes extensive tissue remodelling during the menstrual cycle. Due to their involvement in such processes, several well-characterized matrix metalloproteinases (MMP) have previously been studied in the endometrium. MMP-26 is a newly described matrilysin. We studied MMP-26 mRNA in 39 normal endometrial samples obtained across the menstrual cycle. Tissue distribution and cycle variation was examined using in-situ hybridization, Northern blot analyis and real time PCR. The probes for Northern blot analysis and real time PCR recognized non-overlapping sequences. MMP-26 was localized exclusively in epithelial cells of both glands and the luminal surface. Expression increased during the proliferative phase to a maximum at mid-cycle, then decreased to non-detectable levels in the late secretory and menstrual phases. Expression of MMP-26 mRNA in endometrial tissue explants in vitro required stimulation with both estradiol and progesterone. The tissue content of c-jun mRNA was assayed, since c-jun, as part of the enhancer complex AP-1, may be involved in regulation of MMP-26 gene transcription. The pattern of c-jun expression over the menstrual cycle was similar to that of MMP-26. Epithelial expression in the peri- and post-ovulatory stages of the menstrual cycle suggests the involvement of MMP-26 in reproductive processes. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/308427
- author
- Pilka, Radovan LU ; Whatling, C ; Domanski, Henryk LU ; Hansson, Stefan LU ; Eriksson, P and Casslén, Bertil LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- proteinase, mRNA, MMP-26, c-jun, human endometrium, regulation
- in
- Molecular Human Reproduction
- volume
- 9
- issue
- 5
- pages
- 271 - 277
- publisher
- Oxford University Press
- external identifiers
-
- wos:000183575600005
- pmid:12728020
- scopus:0037635037
- ISSN
- 1460-2407
- DOI
- 10.1093/molehr/gag039
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pathology, (Lund) (013030000), Department of Obstetrics and Gynaecology (Lund) (013018000)
- id
- 6718d6d2-b078-4b4b-beee-2e7d1a2fafc3 (old id 308427)
- alternative location
- http://molehr.oxfordjournals.org/cgi/content/abstract/9/5/271
- date added to LUP
- 2016-04-01 12:23:32
- date last changed
- 2022-01-27 03:05:25
@article{6718d6d2-b078-4b4b-beee-2e7d1a2fafc3, abstract = {{The human endometrium is a dynamic tissue, which undergoes extensive tissue remodelling during the menstrual cycle. Due to their involvement in such processes, several well-characterized matrix metalloproteinases (MMP) have previously been studied in the endometrium. MMP-26 is a newly described matrilysin. We studied MMP-26 mRNA in 39 normal endometrial samples obtained across the menstrual cycle. Tissue distribution and cycle variation was examined using in-situ hybridization, Northern blot analyis and real time PCR. The probes for Northern blot analysis and real time PCR recognized non-overlapping sequences. MMP-26 was localized exclusively in epithelial cells of both glands and the luminal surface. Expression increased during the proliferative phase to a maximum at mid-cycle, then decreased to non-detectable levels in the late secretory and menstrual phases. Expression of MMP-26 mRNA in endometrial tissue explants in vitro required stimulation with both estradiol and progesterone. The tissue content of c-jun mRNA was assayed, since c-jun, as part of the enhancer complex AP-1, may be involved in regulation of MMP-26 gene transcription. The pattern of c-jun expression over the menstrual cycle was similar to that of MMP-26. Epithelial expression in the peri- and post-ovulatory stages of the menstrual cycle suggests the involvement of MMP-26 in reproductive processes.}}, author = {{Pilka, Radovan and Whatling, C and Domanski, Henryk and Hansson, Stefan and Eriksson, P and Casslén, Bertil}}, issn = {{1460-2407}}, keywords = {{proteinase; mRNA; MMP-26; c-jun; human endometrium; regulation}}, language = {{eng}}, number = {{5}}, pages = {{271--277}}, publisher = {{Oxford University Press}}, series = {{Molecular Human Reproduction}}, title = {{Epithelial expression of matrix metalloproteinase-26 is elevated at mid-cycle in the human endometrium}}, url = {{http://dx.doi.org/10.1093/molehr/gag039}}, doi = {{10.1093/molehr/gag039}}, volume = {{9}}, year = {{2003}}, }