Characterization of gamma-tubulin filaments in mammalian cells
(2018) In Biochimica et Biophysica Acta - Molecular Cell Research 1865(1). p.158-171- Abstract
Overexpression of γ-tubulin leads to the formation of filaments, but nothing is known about such filaments with regard to possible presence in cells, structure and probable dynamics. Here, we used mammalian cell lines to investigate the ability of γ-tubulin to form filaments. We found that γ-tubulin produces fibers called γ-tubules in a GTP-dependent manner and that γ-tubules are made up of pericentrin and the γ-tubulin complex proteins 2, 3, 5 and 6. Furthermore, we noted that the number of cells with cytosolic γ-tubules is increased in non-dividing cells. Our experiments showed that γ-tubules are polar structures that have a low regrowth rate compared to microtubules. Also, we observed that γ-tubules were disassembled by treatment... (More)
Overexpression of γ-tubulin leads to the formation of filaments, but nothing is known about such filaments with regard to possible presence in cells, structure and probable dynamics. Here, we used mammalian cell lines to investigate the ability of γ-tubulin to form filaments. We found that γ-tubulin produces fibers called γ-tubules in a GTP-dependent manner and that γ-tubules are made up of pericentrin and the γ-tubulin complex proteins 2, 3, 5 and 6. Furthermore, we noted that the number of cells with cytosolic γ-tubules is increased in non-dividing cells. Our experiments showed that γ-tubules are polar structures that have a low regrowth rate compared to microtubules. Also, we observed that γ-tubules were disassembled by treatment with cold, colcemid, citral dimethyl acetal, dimethyl fumarate or mutation of γ-tubulin GTPase domain, but were increased in number by treatment with taxol or by stable expression of the γ-tubulin1–333 GTPase domain. Our results demonstrate that γ-tubulin forms filaments, and such assembly is facilitated by the GTPase domain of γ-tubulin.
(Less)
- author
- Lindström, Lisa LU and Alvarado-Kristensson, Maria LU
- organization
- publishing date
- 2018-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Filaments, γ-Tubulin, γ-Tubulin ring complex
- in
- Biochimica et Biophysica Acta - Molecular Cell Research
- volume
- 1865
- issue
- 1
- pages
- 14 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:29050966
- scopus:85032352376
- ISSN
- 0167-4889
- DOI
- 10.1016/j.bbamcr.2017.10.008
- language
- English
- LU publication?
- yes
- id
- 309fa67f-1162-4d38-8187-2991a5d0abec
- date added to LUP
- 2017-11-07 09:31:52
- date last changed
- 2024-03-18 00:07:57
@article{309fa67f-1162-4d38-8187-2991a5d0abec,
abstract = {{<p>Overexpression of γ-tubulin leads to the formation of filaments, but nothing is known about such filaments with regard to possible presence in cells, structure and probable dynamics. Here, we used mammalian cell lines to investigate the ability of γ-tubulin to form filaments. We found that γ-tubulin produces fibers called γ-tubules in a GTP-dependent manner and that γ-tubules are made up of pericentrin and the γ-tubulin complex proteins 2, 3, 5 and 6. Furthermore, we noted that the number of cells with cytosolic γ-tubules is increased in non-dividing cells. Our experiments showed that γ-tubules are polar structures that have a low regrowth rate compared to microtubules. Also, we observed that γ-tubules were disassembled by treatment with cold, colcemid, citral dimethyl acetal, dimethyl fumarate or mutation of γ-tubulin GTPase domain, but were increased in number by treatment with taxol or by stable expression of the γ-tubulin<sup>1–333</sup> GTPase domain. Our results demonstrate that γ-tubulin forms filaments, and such assembly is facilitated by the GTPase domain of γ-tubulin.</p>}},
author = {{Lindström, Lisa and Alvarado-Kristensson, Maria}},
issn = {{0167-4889}},
keywords = {{Filaments; γ-Tubulin; γ-Tubulin ring complex}},
language = {{eng}},
month = {{01}},
number = {{1}},
pages = {{158--171}},
publisher = {{Elsevier}},
series = {{Biochimica et Biophysica Acta - Molecular Cell Research}},
title = {{Characterization of gamma-tubulin filaments in mammalian cells}},
url = {{http://dx.doi.org/10.1016/j.bbamcr.2017.10.008}},
doi = {{10.1016/j.bbamcr.2017.10.008}},
volume = {{1865}},
year = {{2018}},
}