Prebiotic-non-digestible oligosaccharides preference of probiotic bifidobacteria and antimicrobial activity against Clostridium difficile.
(2012) In Anaerobe 18(5). p.489-497- Abstract
- Bifidobacterium breve 46, Bifidobacteriumlactis 8:8 and Bifidobacteriumlongum 6:18 and three reference strains B. breve CCUG 24611, B. lactis JCM 10602, and Bifidobacteriumpseudocatenulatum JCM 1200 were examined for acid and bile tolerance, prebiotic utilization and antimicrobial activity against four Clostridium difficile (CD) strains including the hypervirulent strain, PCR ribotype NAP1/027. B. lactis 8:8 and B. lactis JCM 10602 exhibited a high tolerance in MRSC broth with pH 2.5 for 30 min. B. breve 46 and B. lactis 8:8 remained 100% viable in MRSC broth with 5% porcine bile after 4 h. All six strains showed a high prebiotic degrading ability (prebiotic score) with galactooligosaccharides (GOS), isomaltooligosaccharides (IMOS) and... (More)
- Bifidobacterium breve 46, Bifidobacteriumlactis 8:8 and Bifidobacteriumlongum 6:18 and three reference strains B. breve CCUG 24611, B. lactis JCM 10602, and Bifidobacteriumpseudocatenulatum JCM 1200 were examined for acid and bile tolerance, prebiotic utilization and antimicrobial activity against four Clostridium difficile (CD) strains including the hypervirulent strain, PCR ribotype NAP1/027. B. lactis 8:8 and B. lactis JCM 10602 exhibited a high tolerance in MRSC broth with pH 2.5 for 30 min. B. breve 46 and B. lactis 8:8 remained 100% viable in MRSC broth with 5% porcine bile after 4 h. All six strains showed a high prebiotic degrading ability (prebiotic score) with galactooligosaccharides (GOS), isomaltooligosaccharides (IMOS) and lactulose as carbon sources and moderate degradation of fructooligosaccharides (FOS). Xylooligosaccharides (XOS) was metabolized to a greater extent by B. lactis 8:8, B. lactis JCM 10602, B. pseudocatenulatum JCM 1200 and B. longum 6:18 (prebiotic score >50%). All strains exhibited extracellular antimicrobial activity (AMA) against four CD strains including the CD NAP1/027. AMA of B. breve 46, B. lactis 8:8 and B. lactis JCM 10602 strains was mainly ascribed to a combined action of organic acids and heat stable, protease sensitive antimicrobial peptides when cells were grown in MRSC broth with glucose and by acids when grown with five different prebiotic-non-digestible oligosaccharides (NDOs). None of C. difficile strains degraded five prebiotic-NDOs. Whole cells of B. breve 46 and B. lactis 8:8 and their supernatants inhibited the growth and toxin production of the CD NAP1/027 strain. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3124479
- author
- Kondepudi, Kanthi Kiran ; Ambalam, Padma ; Nilsson, Ingrid LU ; Wadström, Torkel LU and Ljungh, Åsa LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Anaerobe
- volume
- 18
- issue
- 5
- pages
- 489 - 497
- publisher
- Elsevier
- external identifiers
-
- wos:000310943200003
- pmid:22940065
- scopus:84867329333
- pmid:22940065
- ISSN
- 1095-8274
- DOI
- 10.1016/j.anaerobe.2012.08.005
- language
- English
- LU publication?
- yes
- id
- f7e2fb52-6cd9-4047-b25f-fd9d52e4b9fe (old id 3124479)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/22940065?dopt=Abstract
- date added to LUP
- 2016-04-04 07:08:11
- date last changed
- 2022-03-30 21:57:03
@article{f7e2fb52-6cd9-4047-b25f-fd9d52e4b9fe,
abstract = {{Bifidobacterium breve 46, Bifidobacteriumlactis 8:8 and Bifidobacteriumlongum 6:18 and three reference strains B. breve CCUG 24611, B. lactis JCM 10602, and Bifidobacteriumpseudocatenulatum JCM 1200 were examined for acid and bile tolerance, prebiotic utilization and antimicrobial activity against four Clostridium difficile (CD) strains including the hypervirulent strain, PCR ribotype NAP1/027. B. lactis 8:8 and B. lactis JCM 10602 exhibited a high tolerance in MRSC broth with pH 2.5 for 30 min. B. breve 46 and B. lactis 8:8 remained 100% viable in MRSC broth with 5% porcine bile after 4 h. All six strains showed a high prebiotic degrading ability (prebiotic score) with galactooligosaccharides (GOS), isomaltooligosaccharides (IMOS) and lactulose as carbon sources and moderate degradation of fructooligosaccharides (FOS). Xylooligosaccharides (XOS) was metabolized to a greater extent by B. lactis 8:8, B. lactis JCM 10602, B. pseudocatenulatum JCM 1200 and B. longum 6:18 (prebiotic score >50%). All strains exhibited extracellular antimicrobial activity (AMA) against four CD strains including the CD NAP1/027. AMA of B. breve 46, B. lactis 8:8 and B. lactis JCM 10602 strains was mainly ascribed to a combined action of organic acids and heat stable, protease sensitive antimicrobial peptides when cells were grown in MRSC broth with glucose and by acids when grown with five different prebiotic-non-digestible oligosaccharides (NDOs). None of C. difficile strains degraded five prebiotic-NDOs. Whole cells of B. breve 46 and B. lactis 8:8 and their supernatants inhibited the growth and toxin production of the CD NAP1/027 strain.}},
author = {{Kondepudi, Kanthi Kiran and Ambalam, Padma and Nilsson, Ingrid and Wadström, Torkel and Ljungh, Åsa}},
issn = {{1095-8274}},
language = {{eng}},
number = {{5}},
pages = {{489--497}},
publisher = {{Elsevier}},
series = {{Anaerobe}},
title = {{Prebiotic-non-digestible oligosaccharides preference of probiotic bifidobacteria and antimicrobial activity against Clostridium difficile.}},
url = {{http://dx.doi.org/10.1016/j.anaerobe.2012.08.005}},
doi = {{10.1016/j.anaerobe.2012.08.005}},
volume = {{18}},
year = {{2012}},
}