Gene expression profiling indicates that immunohistochemical expression of CD40 is a marker of an inflammatory reaction in the tumor stroma of diffuse large B-cell lymphoma
(2012) In Leukemia & Lymphoma 53(9). p.1764-1768- Abstract
- Immunohistochemical expression of CD40 is seen in 60-70% of diffuse large B-cell lymphoma (DLBCL) and is associated with a superior prognosis. By using gene expression profiling we aimed to further explore the underlying mechanisms for this effect. Ninety-eight immunohistochemically defined CD40 positive or negative DLBCL tumors, 63 and 35 respectively, were examined using spotted 55K oligonucleotide arrays. CD40 expressing tumors were characterized by up-regulated expression of genes encoding proteins involved in cell-matrix interactions: collagens, integrin a V, proteoglycans and proteolytic enzymes, and antigen presentation. Immunohistochemistry confirmed that CD40 positive tumors co-express the proinflammatory proteoglycan biglycan (p... (More)
- Immunohistochemical expression of CD40 is seen in 60-70% of diffuse large B-cell lymphoma (DLBCL) and is associated with a superior prognosis. By using gene expression profiling we aimed to further explore the underlying mechanisms for this effect. Ninety-eight immunohistochemically defined CD40 positive or negative DLBCL tumors, 63 and 35 respectively, were examined using spotted 55K oligonucleotide arrays. CD40 expressing tumors were characterized by up-regulated expression of genes encoding proteins involved in cell-matrix interactions: collagens, integrin a V, proteoglycans and proteolytic enzymes, and antigen presentation. Immunohistochemistry confirmed that CD40 positive tumors co-express the proinflammatory proteoglycan biglycan (p = 0.005), which in turn correlates with the amount of infiltrating macrophages and CD4 and CD8 positive T-cells. We postulate that immunohistochemical expression of CD40 mainly reflects the inflammatory status in tumors. A high intratumoral inflammatory reaction may correlate with an increased autologous tumor response, and thereby a better prognosis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3147755
- author
- Fjordén, Karin LU ; Joost, Patrick LU ; Ehinger, Mats LU ; Edén, Patrik LU ; Jerkeman, Mats LU ; Cavallin-Ståhl, Eva LU and Linderoth, Johan LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Diffuse large B-cell lymphoma, CD40, gene expression profiling, tumor, stroma
- in
- Leukemia & Lymphoma
- volume
- 53
- issue
- 9
- pages
- 1764 - 1768
- publisher
- Taylor & Francis
- external identifiers
-
- wos:000307301500022
- scopus:84864988511
- pmid:22335531
- ISSN
- 1042-8194
- DOI
- 10.3109/10428194.2012.666541
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Computational biology and biological physics (000006113), Pathology, (Lund) (013030000), Oncology, MV (013035000)
- id
- 8b22050a-bd37-46ce-8265-680d3b720805 (old id 3147755)
- date added to LUP
- 2016-04-01 14:43:26
- date last changed
- 2024-02-25 18:12:29
@article{8b22050a-bd37-46ce-8265-680d3b720805, abstract = {{Immunohistochemical expression of CD40 is seen in 60-70% of diffuse large B-cell lymphoma (DLBCL) and is associated with a superior prognosis. By using gene expression profiling we aimed to further explore the underlying mechanisms for this effect. Ninety-eight immunohistochemically defined CD40 positive or negative DLBCL tumors, 63 and 35 respectively, were examined using spotted 55K oligonucleotide arrays. CD40 expressing tumors were characterized by up-regulated expression of genes encoding proteins involved in cell-matrix interactions: collagens, integrin a V, proteoglycans and proteolytic enzymes, and antigen presentation. Immunohistochemistry confirmed that CD40 positive tumors co-express the proinflammatory proteoglycan biglycan (p = 0.005), which in turn correlates with the amount of infiltrating macrophages and CD4 and CD8 positive T-cells. We postulate that immunohistochemical expression of CD40 mainly reflects the inflammatory status in tumors. A high intratumoral inflammatory reaction may correlate with an increased autologous tumor response, and thereby a better prognosis.}}, author = {{Fjordén, Karin and Joost, Patrick and Ehinger, Mats and Edén, Patrik and Jerkeman, Mats and Cavallin-Ståhl, Eva and Linderoth, Johan}}, issn = {{1042-8194}}, keywords = {{Diffuse large B-cell lymphoma; CD40; gene expression profiling; tumor; stroma}}, language = {{eng}}, number = {{9}}, pages = {{1764--1768}}, publisher = {{Taylor & Francis}}, series = {{Leukemia & Lymphoma}}, title = {{Gene expression profiling indicates that immunohistochemical expression of CD40 is a marker of an inflammatory reaction in the tumor stroma of diffuse large B-cell lymphoma}}, url = {{http://dx.doi.org/10.3109/10428194.2012.666541}}, doi = {{10.3109/10428194.2012.666541}}, volume = {{53}}, year = {{2012}}, }