Functional importance of individual rRNA 2'-O-ribose methylations revealed by high-resolution phenotyping
(2008) In RNA 14(4). p.56-649- Abstract
- Ribosomal RNAs contain numerous modifications at specific nucleotides. Despite their evolutionary conservation, the functional role of individual 2'-O-ribose methylations in rRNA is not known. A distinct family of small nucleolar RNAs, box C/D snoRNAs, guides the methylating complex to specific rRNA sites. Using a high-resolution phenotyping approach, we characterized 20 box C/D snoRNA gene deletions for altered growth dynamics under a wide array of environmental perturbations, encompassing intraribosomal antibiotics, inhibitors of specific cellular features, as well as general stressors. Ribosome-specific antibiotics generated phenotypes indicating different and long-ranging structural effects of rRNA methylations on the ribosome. For all... (More)
- Ribosomal RNAs contain numerous modifications at specific nucleotides. Despite their evolutionary conservation, the functional role of individual 2'-O-ribose methylations in rRNA is not known. A distinct family of small nucleolar RNAs, box C/D snoRNAs, guides the methylating complex to specific rRNA sites. Using a high-resolution phenotyping approach, we characterized 20 box C/D snoRNA gene deletions for altered growth dynamics under a wide array of environmental perturbations, encompassing intraribosomal antibiotics, inhibitors of specific cellular features, as well as general stressors. Ribosome-specific antibiotics generated phenotypes indicating different and long-ranging structural effects of rRNA methylations on the ribosome. For all studied box C/D snoRNA mutants we uncovered phenotypes to extraribosomal growth inhibitors, most frequently reflected in alteration in growth lag (adaptation time). A number of strains were highly pleiotropic and displayed a great number of sensitive phenotypes, e.g., deletion mutants of snR70 and snR71, which both have clear human homologues, and deletion mutants of snR65 and snR68. Our data indicate that individual rRNA ribose methylations can play either distinct or general roles in the workings of the ribosome. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3217782
- author
- Esguerra, Jonathan LU ; Warringer, J. and Blomberg, A.
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Ribosomal/*chemistry/genetics/*metabolism, Fungal/chemistry/genetics/metabolism, RNA, Phenotype, Nucleic Acid Conformation/drug effects, Methylation, Anti-Bacterial Agents/pharmacology, Fungal, Genes, Ribosomes/drug effects/metabolism, Saccharomyces cerevisiae/genetics/metabolism, Sequence Deletion, Ribose/*chemistry, Small Nucleolar/chemistry/genetics/metabolism
- in
- RNA
- volume
- 14
- issue
- 4
- pages
- 56 - 649
- publisher
- Cold Spring Harbor Laboratory Press (CSHL)
- external identifiers
-
- scopus:41649115487
- pmid:18256246
- ISSN
- 1355-8382
- DOI
- 10.1261/rna.845808
- language
- English
- LU publication?
- no
- id
- 5d88669c-25fd-4e4b-a7a3-6f3b092aac0b (old id 3217782)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/18256246
- http://www.ncbi.nlm.nih.gov/pubmed/18256246
- date added to LUP
- 2016-04-04 09:04:39
- date last changed
- 2022-04-15 21:39:36
@article{5d88669c-25fd-4e4b-a7a3-6f3b092aac0b, abstract = {{Ribosomal RNAs contain numerous modifications at specific nucleotides. Despite their evolutionary conservation, the functional role of individual 2'-O-ribose methylations in rRNA is not known. A distinct family of small nucleolar RNAs, box C/D snoRNAs, guides the methylating complex to specific rRNA sites. Using a high-resolution phenotyping approach, we characterized 20 box C/D snoRNA gene deletions for altered growth dynamics under a wide array of environmental perturbations, encompassing intraribosomal antibiotics, inhibitors of specific cellular features, as well as general stressors. Ribosome-specific antibiotics generated phenotypes indicating different and long-ranging structural effects of rRNA methylations on the ribosome. For all studied box C/D snoRNA mutants we uncovered phenotypes to extraribosomal growth inhibitors, most frequently reflected in alteration in growth lag (adaptation time). A number of strains were highly pleiotropic and displayed a great number of sensitive phenotypes, e.g., deletion mutants of snR70 and snR71, which both have clear human homologues, and deletion mutants of snR65 and snR68. Our data indicate that individual rRNA ribose methylations can play either distinct or general roles in the workings of the ribosome.}}, author = {{Esguerra, Jonathan and Warringer, J. and Blomberg, A.}}, issn = {{1355-8382}}, keywords = {{Ribosomal/*chemistry/genetics/*metabolism; Fungal/chemistry/genetics/metabolism; RNA; Phenotype; Nucleic Acid Conformation/drug effects; Methylation; Anti-Bacterial Agents/pharmacology; Fungal; Genes; Ribosomes/drug effects/metabolism; Saccharomyces cerevisiae/genetics/metabolism; Sequence Deletion; Ribose/*chemistry; Small Nucleolar/chemistry/genetics/metabolism}}, language = {{eng}}, number = {{4}}, pages = {{56--649}}, publisher = {{Cold Spring Harbor Laboratory Press (CSHL)}}, series = {{RNA}}, title = {{Functional importance of individual rRNA 2'-O-ribose methylations revealed by high-resolution phenotyping}}, url = {{http://dx.doi.org/10.1261/rna.845808}}, doi = {{10.1261/rna.845808}}, volume = {{14}}, year = {{2008}}, }