Mitochondrial respiration in human viable platelets-Methodology and influence of gender, age and storage.
(2013) In Mitochondrion 13(1). p.7-14- Abstract
- Studying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source of human viable mitochondria when studying mitochondrial physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondrial respiration using high-resolution respirometry. Further, we aimed to explore the limits of sample size and the impact of storage as well as to establish a wide range of reference data from different... (More)
- Studying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source of human viable mitochondria when studying mitochondrial physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondrial respiration using high-resolution respirometry. Further, we aimed to explore the limits of sample size and the impact of storage as well as to establish a wide range of reference data from different pediatric and adult cohorts. Our results indicate that platelet mitochondria are well suited for ex-vivo analysis with the need for minute sample amounts and excellent reproducibility and stability. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3218800
- author
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Mitochondrion
- volume
- 13
- issue
- 1
- pages
- 7 - 14
- publisher
- Elsevier
- external identifiers
-
- wos:000315757100002
- pmid:23164798
- scopus:84873304542
- pmid:23164798
- ISSN
- 1567-7249
- DOI
- 10.1016/j.mito.2012.11.001
- project
- EU FP Horizon 2020 COST Action CA15203 MITOEAGLE
- language
- English
- LU publication?
- yes
- id
- 8804a031-bacb-4f14-85ec-5041dd839bcb (old id 3218800)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/23164798?dopt=Abstract
- date added to LUP
- 2016-04-01 10:14:52
- date last changed
- 2024-01-21 09:11:19
@article{8804a031-bacb-4f14-85ec-5041dd839bcb, abstract = {{Studying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source of human viable mitochondria when studying mitochondrial physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondrial respiration using high-resolution respirometry. Further, we aimed to explore the limits of sample size and the impact of storage as well as to establish a wide range of reference data from different pediatric and adult cohorts. Our results indicate that platelet mitochondria are well suited for ex-vivo analysis with the need for minute sample amounts and excellent reproducibility and stability.}}, author = {{Sjövall, Fredrik and Ehinger, Johannes and Marelsson, Sigurdur and Morota, Saori and Åsander Frostner, Eleonor and Uchino, Hiroyuki and Lundgren, Johan and Arnbjörnsson, Einar and Hansson, Magnus and Fellman, Vineta and Elmer, Eskil}}, issn = {{1567-7249}}, language = {{eng}}, number = {{1}}, pages = {{7--14}}, publisher = {{Elsevier}}, series = {{Mitochondrion}}, title = {{Mitochondrial respiration in human viable platelets-Methodology and influence of gender, age and storage.}}, url = {{http://dx.doi.org/10.1016/j.mito.2012.11.001}}, doi = {{10.1016/j.mito.2012.11.001}}, volume = {{13}}, year = {{2013}}, }