Haemophilus influenzae Protein F Mediates Binding to Laminin and Human Pulmonary Epithelial Cells.
(2012) In Journal of Infectious Diseases 207(5). p.803-813- Abstract
- The mucosal pathogen non-typeable Haemophilus influenzae (NTHi) adheres to the respiratory epithelium, or in the case of epithelial damage, to the underlying basement membrane and extracellular matrix that amongst other proteins consists of laminin. We have recently identified Protein F, an ABC-transporter involved in NTHi immune evasion. Homology modeling of the Protein F tertiary structure revealed a strong resemblance to the streptococcal laminin-binding proteins Lbp and Lmb. Here, we show that Protein F promotes binding of NTHi to laminin and primary bronchial epithelial cells. Analyses with recombinant proteins and synthetic peptides revealed that the N-terminal part of Protein F contains the host-interacting region. Moreover, Protein... (More)
- The mucosal pathogen non-typeable Haemophilus influenzae (NTHi) adheres to the respiratory epithelium, or in the case of epithelial damage, to the underlying basement membrane and extracellular matrix that amongst other proteins consists of laminin. We have recently identified Protein F, an ABC-transporter involved in NTHi immune evasion. Homology modeling of the Protein F tertiary structure revealed a strong resemblance to the streptococcal laminin-binding proteins Lbp and Lmb. Here, we show that Protein F promotes binding of NTHi to laminin and primary bronchial epithelial cells. Analyses with recombinant proteins and synthetic peptides revealed that the N-terminal part of Protein F contains the host-interacting region. Moreover, Protein F exists in all clinical isolates, and isogenic NTHi Δhpf mutants display significantly reduced binding to laminin and epithelial cells. We thus suggest Protein F as an important and ubiquitous NTHi adhesin. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3347316
- author
- Jalalvand, Farshid LU ; Su, Yu-Ching LU ; Mörgelin, Matthias LU ; Brant, Marta LU ; Hallgren, Oskar LU ; Westergren-Thorsson, Gunilla LU ; Singh, Birendra LU and Riesbeck, Kristian LU
- organization
- publishing date
- 2012-12-10
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Infectious Diseases
- volume
- 207
- issue
- 5
- pages
- 803 - 813
- publisher
- Oxford University Press
- external identifiers
-
- wos:000314894600014
- pmid:23230060
- scopus:84873684522
- pmid:23230060
- ISSN
- 1537-6613
- DOI
- 10.1093/infdis/jis754
- language
- English
- LU publication?
- yes
- id
- 10b2a626-7c39-4109-88ca-5e897f71bc73 (old id 3347316)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/23230060?dopt=Abstract
- date added to LUP
- 2016-04-01 13:24:01
- date last changed
- 2024-04-10 05:05:10
@article{10b2a626-7c39-4109-88ca-5e897f71bc73, abstract = {{The mucosal pathogen non-typeable Haemophilus influenzae (NTHi) adheres to the respiratory epithelium, or in the case of epithelial damage, to the underlying basement membrane and extracellular matrix that amongst other proteins consists of laminin. We have recently identified Protein F, an ABC-transporter involved in NTHi immune evasion. Homology modeling of the Protein F tertiary structure revealed a strong resemblance to the streptococcal laminin-binding proteins Lbp and Lmb. Here, we show that Protein F promotes binding of NTHi to laminin and primary bronchial epithelial cells. Analyses with recombinant proteins and synthetic peptides revealed that the N-terminal part of Protein F contains the host-interacting region. Moreover, Protein F exists in all clinical isolates, and isogenic NTHi Δhpf mutants display significantly reduced binding to laminin and epithelial cells. We thus suggest Protein F as an important and ubiquitous NTHi adhesin.}}, author = {{Jalalvand, Farshid and Su, Yu-Ching and Mörgelin, Matthias and Brant, Marta and Hallgren, Oskar and Westergren-Thorsson, Gunilla and Singh, Birendra and Riesbeck, Kristian}}, issn = {{1537-6613}}, language = {{eng}}, month = {{12}}, number = {{5}}, pages = {{803--813}}, publisher = {{Oxford University Press}}, series = {{Journal of Infectious Diseases}}, title = {{Haemophilus influenzae Protein F Mediates Binding to Laminin and Human Pulmonary Epithelial Cells.}}, url = {{https://lup.lub.lu.se/search/files/3346218/3461229.pdf}}, doi = {{10.1093/infdis/jis754}}, volume = {{207}}, year = {{2012}}, }