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Sulindac induces apoptosis, inhibits proliferation and activates caspase-3 in Hep G2 cells

Liu, Jian-Jun LU ; Wang, Junyang LU ; Hertervig, E ; Nilsson, A and Duan, Rui-Dong LU (2002) In Anticancer research 22(1A). p.263-266
Abstract
Background: It has recently been reported that sulindac has an apoptotic effect on KYN-2 cells, an undifferentiated hepatoma cell line. The present work investigates whether sulindac also has an apoptotic effect on well-differentiated hepatoma cells and what its potential mechanism might be. Materials and Methods: Hep G2 cells were treated with sulindac at different concentrations. Apoptosis rate, cell proliferation and 3H-thymidine incorporation were measured. The activities of caspase-3, acid and neutral sphingomyelinase and the changes of sphingomyelin content were also assayed. Results: Sulindac dose-dependently induced apoptosis in Hep G2 cells; both sulindac sulfone and sulfide had similar effects. The apoptosis was accompanied by an... (More)
Background: It has recently been reported that sulindac has an apoptotic effect on KYN-2 cells, an undifferentiated hepatoma cell line. The present work investigates whether sulindac also has an apoptotic effect on well-differentiated hepatoma cells and what its potential mechanism might be. Materials and Methods: Hep G2 cells were treated with sulindac at different concentrations. Apoptosis rate, cell proliferation and 3H-thymidine incorporation were measured. The activities of caspase-3, acid and neutral sphingomyelinase and the changes of sphingomyelin content were also assayed. Results: Sulindac dose-dependently induced apoptosis in Hep G2 cells; both sulindac sulfone and sulfide had similar effects. The apoptosis was accompanied by an increase 3 of caspase-3 activity and a decrease of cell proliferation and H-3-thymidine incorporation. No significant change could be observed for the activity of sphingomyelinase and sphingomyelin content. Conclusion: Sulindac induces apoptosis and inhibits proliferation in Hep G2 cells. The effect may, be mediated by, a pathway related to caspase-3 activation but independent of sphingomyelin metabolism. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
sphingomyelinase, caspase-3, proliferation, sulindac, apoptosis
in
Anticancer research
volume
22
issue
1A
pages
263 - 266
publisher
International Institute of Cancer Research
external identifiers
  • wos:000175351900043
  • scopus:0036244224
ISSN
1791-7530
language
English
LU publication?
yes
id
127dbfbe-5479-46e5-9f82-6fb3ecb03a58 (old id 338731)
date added to LUP
2016-04-01 12:32:51
date last changed
2024-01-09 00:24:07
@article{127dbfbe-5479-46e5-9f82-6fb3ecb03a58,
  abstract     = {{Background: It has recently been reported that sulindac has an apoptotic effect on KYN-2 cells, an undifferentiated hepatoma cell line. The present work investigates whether sulindac also has an apoptotic effect on well-differentiated hepatoma cells and what its potential mechanism might be. Materials and Methods: Hep G2 cells were treated with sulindac at different concentrations. Apoptosis rate, cell proliferation and 3H-thymidine incorporation were measured. The activities of caspase-3, acid and neutral sphingomyelinase and the changes of sphingomyelin content were also assayed. Results: Sulindac dose-dependently induced apoptosis in Hep G2 cells; both sulindac sulfone and sulfide had similar effects. The apoptosis was accompanied by an increase 3 of caspase-3 activity and a decrease of cell proliferation and H-3-thymidine incorporation. No significant change could be observed for the activity of sphingomyelinase and sphingomyelin content. Conclusion: Sulindac induces apoptosis and inhibits proliferation in Hep G2 cells. The effect may, be mediated by, a pathway related to caspase-3 activation but independent of sphingomyelin metabolism.}},
  author       = {{Liu, Jian-Jun and Wang, Junyang and Hertervig, E and Nilsson, A and Duan, Rui-Dong}},
  issn         = {{1791-7530}},
  keywords     = {{sphingomyelinase; caspase-3; proliferation; sulindac; apoptosis}},
  language     = {{eng}},
  number       = {{1A}},
  pages        = {{263--266}},
  publisher    = {{International Institute of Cancer Research}},
  series       = {{Anticancer research}},
  title        = {{Sulindac induces apoptosis, inhibits proliferation and activates caspase-3 in Hep G2 cells}},
  volume       = {{22}},
  year         = {{2002}},
}