Sulindac induces apoptosis, inhibits proliferation and activates caspase-3 in Hep G2 cells
(2002) In Anticancer research 22(1A). p.263-266- Abstract
- Background: It has recently been reported that sulindac has an apoptotic effect on KYN-2 cells, an undifferentiated hepatoma cell line. The present work investigates whether sulindac also has an apoptotic effect on well-differentiated hepatoma cells and what its potential mechanism might be. Materials and Methods: Hep G2 cells were treated with sulindac at different concentrations. Apoptosis rate, cell proliferation and 3H-thymidine incorporation were measured. The activities of caspase-3, acid and neutral sphingomyelinase and the changes of sphingomyelin content were also assayed. Results: Sulindac dose-dependently induced apoptosis in Hep G2 cells; both sulindac sulfone and sulfide had similar effects. The apoptosis was accompanied by an... (More)
- Background: It has recently been reported that sulindac has an apoptotic effect on KYN-2 cells, an undifferentiated hepatoma cell line. The present work investigates whether sulindac also has an apoptotic effect on well-differentiated hepatoma cells and what its potential mechanism might be. Materials and Methods: Hep G2 cells were treated with sulindac at different concentrations. Apoptosis rate, cell proliferation and 3H-thymidine incorporation were measured. The activities of caspase-3, acid and neutral sphingomyelinase and the changes of sphingomyelin content were also assayed. Results: Sulindac dose-dependently induced apoptosis in Hep G2 cells; both sulindac sulfone and sulfide had similar effects. The apoptosis was accompanied by an increase 3 of caspase-3 activity and a decrease of cell proliferation and H-3-thymidine incorporation. No significant change could be observed for the activity of sphingomyelinase and sphingomyelin content. Conclusion: Sulindac induces apoptosis and inhibits proliferation in Hep G2 cells. The effect may, be mediated by, a pathway related to caspase-3 activation but independent of sphingomyelin metabolism. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/338731
- author
- Liu, Jian-Jun LU ; Wang, Junyang LU ; Hertervig, E ; Nilsson, A and Duan, Rui-Dong LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- sphingomyelinase, caspase-3, proliferation, sulindac, apoptosis
- in
- Anticancer research
- volume
- 22
- issue
- 1A
- pages
- 263 - 266
- publisher
- International Institute of Cancer Research
- external identifiers
-
- wos:000175351900043
- scopus:0036244224
- ISSN
- 1791-7530
- language
- English
- LU publication?
- yes
- id
- 127dbfbe-5479-46e5-9f82-6fb3ecb03a58 (old id 338731)
- date added to LUP
- 2016-04-01 12:32:51
- date last changed
- 2024-01-09 00:24:07
@article{127dbfbe-5479-46e5-9f82-6fb3ecb03a58, abstract = {{Background: It has recently been reported that sulindac has an apoptotic effect on KYN-2 cells, an undifferentiated hepatoma cell line. The present work investigates whether sulindac also has an apoptotic effect on well-differentiated hepatoma cells and what its potential mechanism might be. Materials and Methods: Hep G2 cells were treated with sulindac at different concentrations. Apoptosis rate, cell proliferation and 3H-thymidine incorporation were measured. The activities of caspase-3, acid and neutral sphingomyelinase and the changes of sphingomyelin content were also assayed. Results: Sulindac dose-dependently induced apoptosis in Hep G2 cells; both sulindac sulfone and sulfide had similar effects. The apoptosis was accompanied by an increase 3 of caspase-3 activity and a decrease of cell proliferation and H-3-thymidine incorporation. No significant change could be observed for the activity of sphingomyelinase and sphingomyelin content. Conclusion: Sulindac induces apoptosis and inhibits proliferation in Hep G2 cells. The effect may, be mediated by, a pathway related to caspase-3 activation but independent of sphingomyelin metabolism.}}, author = {{Liu, Jian-Jun and Wang, Junyang and Hertervig, E and Nilsson, A and Duan, Rui-Dong}}, issn = {{1791-7530}}, keywords = {{sphingomyelinase; caspase-3; proliferation; sulindac; apoptosis}}, language = {{eng}}, number = {{1A}}, pages = {{263--266}}, publisher = {{International Institute of Cancer Research}}, series = {{Anticancer research}}, title = {{Sulindac induces apoptosis, inhibits proliferation and activates caspase-3 in Hep G2 cells}}, volume = {{22}}, year = {{2002}}, }