Gold nanoparticles explore cells : cellular uptake and their use as intracellular probes
(2014) In Methods 68(2). p.63-354- Abstract
Understanding uptake of nanomaterials by cells and their use for intracellular sensing is important for studying their interaction and toxicology as well as for obtaining new biological insight. Here, we investigate cellular uptake and intracellular dynamics of gold nanoparticles and demonstrate their use in reporting chemical information from the endocytotic pathway and cytoplasm. The intracellular gold nanoparticles serve as probes for surface-enhanced Raman spectroscopy (SERS) allowing for biochemical characterisation of their local environment. In particular, in this work we compare intracellular SERS using non-functionalised and functionalised nanoparticles in their ability to segregate different but closely related cell... (More)
Understanding uptake of nanomaterials by cells and their use for intracellular sensing is important for studying their interaction and toxicology as well as for obtaining new biological insight. Here, we investigate cellular uptake and intracellular dynamics of gold nanoparticles and demonstrate their use in reporting chemical information from the endocytotic pathway and cytoplasm. The intracellular gold nanoparticles serve as probes for surface-enhanced Raman spectroscopy (SERS) allowing for biochemical characterisation of their local environment. In particular, in this work we compare intracellular SERS using non-functionalised and functionalised nanoparticles in their ability to segregate different but closely related cell phenotypes. The results indicate that functionalised gold nanoparticles are more efficient in distinguishing between different types of cells. Our studies pave the way for understanding the uptake of gold nanoparticles and their utilisation for SERS to give rise to a greater biochemical understanding in cell-based therapies.
(Less)
- author
- Huefner, Anna ; Septiadi, Dedy ; Wilts, Bodo D ; Patel, Imran I ; Kuan, Wei-Li ; Fragniere, Alexandra ; Barker, Roger A LU and Mahajan, Sumeet
- publishing date
- 2014-07-01
- type
- Contribution to journal
- publication status
- published
- keywords
- Cell Tracking, Cytoplasm, Endocytosis, Gold, Humans, Imaging, Three-Dimensional, Metal Nanoparticles, Spectrum Analysis, Raman, Journal Article
- in
- Methods
- volume
- 68
- issue
- 2
- pages
- 63 - 354
- publisher
- Elsevier
- external identifiers
-
- pmid:24583117
- scopus:84902329592
- ISSN
- 1095-9130
- DOI
- 10.1016/j.ymeth.2014.02.006
- language
- English
- LU publication?
- no
- id
- 33d24491-a2fd-47a6-9864-bf8ae3ac9fde
- date added to LUP
- 2016-11-24 15:14:04
- date last changed
- 2024-09-07 02:39:10
@article{33d24491-a2fd-47a6-9864-bf8ae3ac9fde, abstract = {{<p>Understanding uptake of nanomaterials by cells and their use for intracellular sensing is important for studying their interaction and toxicology as well as for obtaining new biological insight. Here, we investigate cellular uptake and intracellular dynamics of gold nanoparticles and demonstrate their use in reporting chemical information from the endocytotic pathway and cytoplasm. The intracellular gold nanoparticles serve as probes for surface-enhanced Raman spectroscopy (SERS) allowing for biochemical characterisation of their local environment. In particular, in this work we compare intracellular SERS using non-functionalised and functionalised nanoparticles in their ability to segregate different but closely related cell phenotypes. The results indicate that functionalised gold nanoparticles are more efficient in distinguishing between different types of cells. Our studies pave the way for understanding the uptake of gold nanoparticles and their utilisation for SERS to give rise to a greater biochemical understanding in cell-based therapies.</p>}}, author = {{Huefner, Anna and Septiadi, Dedy and Wilts, Bodo D and Patel, Imran I and Kuan, Wei-Li and Fragniere, Alexandra and Barker, Roger A and Mahajan, Sumeet}}, issn = {{1095-9130}}, keywords = {{Cell Tracking; Cytoplasm; Endocytosis; Gold; Humans; Imaging, Three-Dimensional; Metal Nanoparticles; Spectrum Analysis, Raman; Journal Article}}, language = {{eng}}, month = {{07}}, number = {{2}}, pages = {{63--354}}, publisher = {{Elsevier}}, series = {{Methods}}, title = {{Gold nanoparticles explore cells : cellular uptake and their use as intracellular probes}}, url = {{http://dx.doi.org/10.1016/j.ymeth.2014.02.006}}, doi = {{10.1016/j.ymeth.2014.02.006}}, volume = {{68}}, year = {{2014}}, }