Mechanism of murine V gamma 1(+) gamma delta T cell-mediated innate immune response against Listeria monocytogenes infection
(2002) In European Journal of Immunology 32(4). p.928-935- Abstract
- Murine gammadelta T cells participate in innate immune response against infection of the intracellular bacterium Listeria monocytogenes. In the present report, we analyzed the mechanism of the gammadelta T cell-mediated response against L. monocytogenes infection. gammadelta T cell-enriched spleen cells of L. monocytogenes-infected mice produced IFN-gamma in vitro in response to L. monocytogenes-infected spleen cells, The IFN-gamma production was abrogated by depletion of Vgamma1(+) gammadelta T cells. IFN-gamma production of the Vy1(+) gammadelta T cells in response to L. monocytogenes-infected spleen cells required IL-12. However, addition of Fab fragment of anti-TCR gammadelta monoclonal antibodies (mAb) failed to block the response,... (More)
- Murine gammadelta T cells participate in innate immune response against infection of the intracellular bacterium Listeria monocytogenes. In the present report, we analyzed the mechanism of the gammadelta T cell-mediated response against L. monocytogenes infection. gammadelta T cell-enriched spleen cells of L. monocytogenes-infected mice produced IFN-gamma in vitro in response to L. monocytogenes-infected spleen cells, The IFN-gamma production was abrogated by depletion of Vgamma1(+) gammadelta T cells. IFN-gamma production of the Vy1(+) gammadelta T cells in response to L. monocytogenes-infected spleen cells required IL-12. However, addition of Fab fragment of anti-TCR gammadelta monoclonal antibodies (mAb) failed to block the response, suggesting that the response requires no TCR-mediated antigen recognition. Interestingly, Vgamma1(+) gammadelta T cells of naive mice also produced IFN-gamma in response to L. monocytogenes-infected spleen cells in an IL-12-dependent manner. Furthermore, the IL-12 receptor (IL-12R) gene was expressed on the Vgamma1(+) gammadelta T cells of naive mice as well as those of L. monocytogenes-infected mice although naive alphabeta T cells lack IL-12R expression. All the results suggest that the Vgamma1(+) gammadelta T cells participate in immune surveillance against intracellular bacterial infection through quick production of IFN-gamma in response to infection-induced IL-12 without antigen-driven clonal expansion and maturation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/340360
- author
- Matsuzaki, G ; Yamada, Hisakata LU ; Kishihara, K ; Yoshikai, Y and Nomoto, K
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- gamma delta T cell, Listeria monocytogenes, IL-12, IFN-gamma
- in
- European Journal of Immunology
- volume
- 32
- issue
- 4
- pages
- 928 - 935
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:11920558
- wos:000174968700002
- scopus:0036010964
- ISSN
- 1521-4141
- DOI
- 10.1002/1521-4141(200204)32:4<928::AID-IMMU928>3.0.CO;2-I
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Medical Inflammation Research (013212019)
- id
- 6b116b49-6ae0-49ab-acfb-a673d262e16d (old id 340360)
- date added to LUP
- 2016-04-01 11:46:54
- date last changed
- 2022-01-26 18:07:55
@article{6b116b49-6ae0-49ab-acfb-a673d262e16d,
abstract = {{Murine gammadelta T cells participate in innate immune response against infection of the intracellular bacterium Listeria monocytogenes. In the present report, we analyzed the mechanism of the gammadelta T cell-mediated response against L. monocytogenes infection. gammadelta T cell-enriched spleen cells of L. monocytogenes-infected mice produced IFN-gamma in vitro in response to L. monocytogenes-infected spleen cells, The IFN-gamma production was abrogated by depletion of Vgamma1(+) gammadelta T cells. IFN-gamma production of the Vy1(+) gammadelta T cells in response to L. monocytogenes-infected spleen cells required IL-12. However, addition of Fab fragment of anti-TCR gammadelta monoclonal antibodies (mAb) failed to block the response, suggesting that the response requires no TCR-mediated antigen recognition. Interestingly, Vgamma1(+) gammadelta T cells of naive mice also produced IFN-gamma in response to L. monocytogenes-infected spleen cells in an IL-12-dependent manner. Furthermore, the IL-12 receptor (IL-12R) gene was expressed on the Vgamma1(+) gammadelta T cells of naive mice as well as those of L. monocytogenes-infected mice although naive alphabeta T cells lack IL-12R expression. All the results suggest that the Vgamma1(+) gammadelta T cells participate in immune surveillance against intracellular bacterial infection through quick production of IFN-gamma in response to infection-induced IL-12 without antigen-driven clonal expansion and maturation.}},
author = {{Matsuzaki, G and Yamada, Hisakata and Kishihara, K and Yoshikai, Y and Nomoto, K}},
issn = {{1521-4141}},
keywords = {{gamma delta T cell; Listeria monocytogenes; IL-12; IFN-gamma}},
language = {{eng}},
number = {{4}},
pages = {{928--935}},
publisher = {{John Wiley & Sons Inc.}},
series = {{European Journal of Immunology}},
title = {{Mechanism of murine V gamma 1(+) gamma delta T cell-mediated innate immune response against Listeria monocytogenes infection}},
url = {{http://dx.doi.org/10.1002/1521-4141(200204)32:4<928::AID-IMMU928>3.0.CO;2-I}},
doi = {{10.1002/1521-4141(200204)32:4<928::AID-IMMU928>3.0.CO;2-I}},
volume = {{32}},
year = {{2002}},
}