Comparative analysis of the mouse and human genes (Matn2 and MATN2) for matrilin-2, a filament-forming protein widely distributed in extracellular matrices
(2002) In Matrix Biology 21(2). p.163-174- Abstract
- We previously identified matrilin-2 (MATN2), the largest member of the novel family of matrilins. These filament-forming adapter proteins expressed in a distinct, but partially overlapping, pattern in all tissues were implicated in the organization of the extracellular matrix. Matrilin-2 functions in a great variety of tissues. Here, we present the genomic organization of the highly conserved mouse and human MATN2 loci, which cover > 100 kb and 167.167 kb genomic regions, respectively, and are composed of 19 exons. RT-PCR analysis revealed that alternative transcripts with identical protein coding regions are transcribed from two promoters in both species. The upstream, housekeeping type promoter is functional in all tissues and cell... (More)
- We previously identified matrilin-2 (MATN2), the largest member of the novel family of matrilins. These filament-forming adapter proteins expressed in a distinct, but partially overlapping, pattern in all tissues were implicated in the organization of the extracellular matrix. Matrilin-2 functions in a great variety of tissues. Here, we present the genomic organization of the highly conserved mouse and human MATN2 loci, which cover > 100 kb and 167.167 kb genomic regions, respectively, and are composed of 19 exons. RT-PCR analysis revealed that alternative transcripts with identical protein coding regions are transcribed from two promoters in both species. The upstream, housekeeping type promoter is functional in all tissues and cell types tested. The activity of the downstream, TATA-like promoter preceded with putative motifs for the homeobox transcription factor PRRX2 is restricted to embryonic fibroblasts and certain cell lines. The oligomerization module is split by an U12-type AT-AC intron found in conserved position in all four matrilin genes. We assigned Matn2 to mouse chromosome 15, linked to Trhr and Sntb1 in a region synthenic to human chromosome 8q22-24. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/341984
- author
- Mates, L ; Korpos, E ; Deak, F ; Liu, ZQ ; Beier, DR ; Aszodi, Attila LU and Kiss, I
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- PRRX2 motifs, alternative promoters, introns, AT-AC, genomic organization, extracellular matrix, adapter protein
- in
- Matrix Biology
- volume
- 21
- issue
- 2
- pages
- 163 - 174
- publisher
- Elsevier
- external identifiers
-
- wos:000174426100005
- scopus:0036168619
- ISSN
- 1569-1802
- DOI
- 10.1016/S0945-053X(01)00194-9
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Experimental Pathology (013031100), Pathology, (Lund) (013030000)
- id
- 5c7568ee-7915-4752-994d-e78d700aa483 (old id 341984)
- date added to LUP
- 2016-04-01 15:36:17
- date last changed
- 2022-01-28 06:11:03
@article{5c7568ee-7915-4752-994d-e78d700aa483, abstract = {{We previously identified matrilin-2 (MATN2), the largest member of the novel family of matrilins. These filament-forming adapter proteins expressed in a distinct, but partially overlapping, pattern in all tissues were implicated in the organization of the extracellular matrix. Matrilin-2 functions in a great variety of tissues. Here, we present the genomic organization of the highly conserved mouse and human MATN2 loci, which cover > 100 kb and 167.167 kb genomic regions, respectively, and are composed of 19 exons. RT-PCR analysis revealed that alternative transcripts with identical protein coding regions are transcribed from two promoters in both species. The upstream, housekeeping type promoter is functional in all tissues and cell types tested. The activity of the downstream, TATA-like promoter preceded with putative motifs for the homeobox transcription factor PRRX2 is restricted to embryonic fibroblasts and certain cell lines. The oligomerization module is split by an U12-type AT-AC intron found in conserved position in all four matrilin genes. We assigned Matn2 to mouse chromosome 15, linked to Trhr and Sntb1 in a region synthenic to human chromosome 8q22-24.}}, author = {{Mates, L and Korpos, E and Deak, F and Liu, ZQ and Beier, DR and Aszodi, Attila and Kiss, I}}, issn = {{1569-1802}}, keywords = {{PRRX2 motifs; alternative promoters; introns; AT-AC; genomic organization; extracellular matrix; adapter protein}}, language = {{eng}}, number = {{2}}, pages = {{163--174}}, publisher = {{Elsevier}}, series = {{Matrix Biology}}, title = {{Comparative analysis of the mouse and human genes (Matn2 and MATN2) for matrilin-2, a filament-forming protein widely distributed in extracellular matrices}}, url = {{http://dx.doi.org/10.1016/S0945-053X(01)00194-9}}, doi = {{10.1016/S0945-053X(01)00194-9}}, volume = {{21}}, year = {{2002}}, }