Localization of phosphatidylserine binding sites to structural domains of factor X-a
(2002) In Journal of Biological Chemistry 277(3). p.1855-1863- Abstract
- Binding of short chain phosphatidylserine (C6PS) enhances the proteolytic activity of factor X-a by 60-fold (Koppaka, V., Wang, J., Banerjee, M., and Lentz, B. R. (1996) Biochemistry 35, 7482-7491). In the present study, we locate three C6PS binding sites to different domains of factor X-a using a combination of activity, circular dichroism, fluorescence, and equilibrium dialysis measurements on proteolytic and biosynthetic fragments of factor X-a. Our results demonstrate that the structural responses of human and bovine factor X. to C6PS binding are somewhat different. Despite this difference, data obtained with fragments from both human and bovine factor X-a are consistent with a common hypothesis for the location of C6PS binding sites... (More)
- Binding of short chain phosphatidylserine (C6PS) enhances the proteolytic activity of factor X-a by 60-fold (Koppaka, V., Wang, J., Banerjee, M., and Lentz, B. R. (1996) Biochemistry 35, 7482-7491). In the present study, we locate three C6PS binding sites to different domains of factor X-a using a combination of activity, circular dichroism, fluorescence, and equilibrium dialysis measurements on proteolytic and biosynthetic fragments of factor X-a. Our results demonstrate that the structural responses of human and bovine factor X. to C6PS binding are somewhat different. Despite this difference, data obtained with fragments from both human and bovine factor X-a are consistent with a common hypothesis for the location of C6PS binding sites to different structural domains. First, the gamma-carboxyglutamic acid (Gla) domain binds C6PS only in the absence of Ca2+ (k(d) similar to 1 mm), although this PS site does not influence the functional response of factor X-a. Second, a Ca2+-dependent binding site is in the epidermal growth factor domains (EGF(NC)) that are linked by Ca2+ and C6PS binding to the Gla domain. This site appears to be the lipid regulatory site of factor X-a. Third, a Ca2+-requiring site seems to be in the EGF(C)-catalytic domain. This site appears not to be a lipid regulatory site but rather to share residues with the substrate recognition site. Finally, the full functional response to C6PS requires linkage of the Gla, EGF(NC), and catalytic domains in the presence of Ca2+, meaning that PS regulation of factor X-a involves linkage between widely separated parts of the protein. (Less)
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https://lup.lub.lu.se/record/344437
- author
- Srivastava, A ; Wang, JF ; Majumder, R ; Rezaie, AR ; Stenflo, Johan LU ; Esmon, CT and Lentz, BR
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Biological Chemistry
- volume
- 277
- issue
- 3
- pages
- 1855 - 1863
- publisher
- American Society for Biochemistry and Molecular Biology
- external identifiers
-
- wos:000173421300032
- pmid:11707438
- scopus:0037127212
- ISSN
- 1083-351X
- DOI
- 10.1074/jbc.M105697200
- language
- English
- LU publication?
- yes
- id
- d140eeb6-3307-4b82-bc7b-f818d04ff64d (old id 344437)
- date added to LUP
- 2016-04-01 12:33:41
- date last changed
- 2022-01-27 06:44:10
@article{d140eeb6-3307-4b82-bc7b-f818d04ff64d,
abstract = {{Binding of short chain phosphatidylserine (C6PS) enhances the proteolytic activity of factor X-a by 60-fold (Koppaka, V., Wang, J., Banerjee, M., and Lentz, B. R. (1996) Biochemistry 35, 7482-7491). In the present study, we locate three C6PS binding sites to different domains of factor X-a using a combination of activity, circular dichroism, fluorescence, and equilibrium dialysis measurements on proteolytic and biosynthetic fragments of factor X-a. Our results demonstrate that the structural responses of human and bovine factor X. to C6PS binding are somewhat different. Despite this difference, data obtained with fragments from both human and bovine factor X-a are consistent with a common hypothesis for the location of C6PS binding sites to different structural domains. First, the gamma-carboxyglutamic acid (Gla) domain binds C6PS only in the absence of Ca2+ (k(d) similar to 1 mm), although this PS site does not influence the functional response of factor X-a. Second, a Ca2+-dependent binding site is in the epidermal growth factor domains (EGF(NC)) that are linked by Ca2+ and C6PS binding to the Gla domain. This site appears to be the lipid regulatory site of factor X-a. Third, a Ca2+-requiring site seems to be in the EGF(C)-catalytic domain. This site appears not to be a lipid regulatory site but rather to share residues with the substrate recognition site. Finally, the full functional response to C6PS requires linkage of the Gla, EGF(NC), and catalytic domains in the presence of Ca2+, meaning that PS regulation of factor X-a involves linkage between widely separated parts of the protein.}},
author = {{Srivastava, A and Wang, JF and Majumder, R and Rezaie, AR and Stenflo, Johan and Esmon, CT and Lentz, BR}},
issn = {{1083-351X}},
language = {{eng}},
number = {{3}},
pages = {{1855--1863}},
publisher = {{American Society for Biochemistry and Molecular Biology}},
series = {{Journal of Biological Chemistry}},
title = {{Localization of phosphatidylserine binding sites to structural domains of factor X-a}},
url = {{http://dx.doi.org/10.1074/jbc.M105697200}},
doi = {{10.1074/jbc.M105697200}},
volume = {{277}},
year = {{2002}},
}