Lactobacilli express cell surface proteins which mediate binding of immobilized collagen and fibronectin
(2002) In FEMS Microbiology Letters 206(1). p.31-37- Abstract
- Binding of immobilized collagen-I (Cn-I) and fibronectin (Fn) by Lactobacillus acidophilus CRL 639 depends on cell-surface proteins. Capsule formation during the stationary growth phase has a negative effect on adherence of Cn-I and Fn. However, cells from the exponential growth phase. which produce no capsule, exhibit maximal binding. Binding is sensitive to trypsin, proteinase K, pronase E, and heat. Gelatin and soluble Cn-I partially inhibit binding of Cn-I although various proteins, sugars and amino acids do not affect binding to Fn. These results indicate that protein-protein interactions mediate adhesion to extracellular matrix proteins. SDS-PAGE and Western blot analyses of surface proteins revealed that several proteins including... (More)
- Binding of immobilized collagen-I (Cn-I) and fibronectin (Fn) by Lactobacillus acidophilus CRL 639 depends on cell-surface proteins. Capsule formation during the stationary growth phase has a negative effect on adherence of Cn-I and Fn. However, cells from the exponential growth phase. which produce no capsule, exhibit maximal binding. Binding is sensitive to trypsin, proteinase K, pronase E, and heat. Gelatin and soluble Cn-I partially inhibit binding of Cn-I although various proteins, sugars and amino acids do not affect binding to Fn. These results indicate that protein-protein interactions mediate adhesion to extracellular matrix proteins. SDS-PAGE and Western blot analyses of surface proteins revealed that several proteins including the major 43-kDa protein of the S-layer are expressed. Monoclonal antibodies showed that Fn binds to a 15-kDa protein, while Cn-I binds to proteins of 45 and 58 kDa. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/345903
- author
- Lorca, G ; Torino, MI ; de Valdez, GF and Ljungh, Åsa LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- immunoblot, polysaccharide, fibronectin binding, Lactobacillus, collagen binding, protein-protein interaction
- in
- FEMS Microbiology Letters
- volume
- 206
- issue
- 1
- pages
- 31 - 37
- publisher
- Oxford University Press
- external identifiers
-
- wos:000173284600005
- pmid:11786253
- scopus:0037005874
- ISSN
- 1574-6968
- DOI
- 10.1016/S0378-1097(01)00512-2
- language
- English
- LU publication?
- yes
- id
- 855c067c-6d6c-4d37-8cfd-18fda462d29f (old id 345903)
- date added to LUP
- 2016-04-01 16:16:43
- date last changed
- 2022-03-07 04:49:56
@article{855c067c-6d6c-4d37-8cfd-18fda462d29f, abstract = {{Binding of immobilized collagen-I (Cn-I) and fibronectin (Fn) by Lactobacillus acidophilus CRL 639 depends on cell-surface proteins. Capsule formation during the stationary growth phase has a negative effect on adherence of Cn-I and Fn. However, cells from the exponential growth phase. which produce no capsule, exhibit maximal binding. Binding is sensitive to trypsin, proteinase K, pronase E, and heat. Gelatin and soluble Cn-I partially inhibit binding of Cn-I although various proteins, sugars and amino acids do not affect binding to Fn. These results indicate that protein-protein interactions mediate adhesion to extracellular matrix proteins. SDS-PAGE and Western blot analyses of surface proteins revealed that several proteins including the major 43-kDa protein of the S-layer are expressed. Monoclonal antibodies showed that Fn binds to a 15-kDa protein, while Cn-I binds to proteins of 45 and 58 kDa.}}, author = {{Lorca, G and Torino, MI and de Valdez, GF and Ljungh, Åsa}}, issn = {{1574-6968}}, keywords = {{immunoblot; polysaccharide; fibronectin binding; Lactobacillus; collagen binding; protein-protein interaction}}, language = {{eng}}, number = {{1}}, pages = {{31--37}}, publisher = {{Oxford University Press}}, series = {{FEMS Microbiology Letters}}, title = {{Lactobacilli express cell surface proteins which mediate binding of immobilized collagen and fibronectin}}, url = {{http://dx.doi.org/10.1016/S0378-1097(01)00512-2}}, doi = {{10.1016/S0378-1097(01)00512-2}}, volume = {{206}}, year = {{2002}}, }