New Set-up for Capillary Isoelectric Focusing in Uncoated Capillaries
(1998) In Journal of Chromatography A 813(2). p.349-360- Abstract
- Substituted aminomethylphenol dyes, low-molecular-mass isoelectric point (pI ) markers and hemoglobin samples from normal individuals and diabetic patients were used to test a new set-up of capillary isoelectric focusing (cIEF) in uncoated capillaries. In previous cIEF methods, a mixture of sample components and carrier ampholytes was applied in the capillary and analyzed. In the new set-up a fractionated injection protocol is used to apply a ‘sandwich’ ampholyte–sample–ampholyte plug in the capillary for analysis. This new set-up allows the separation of amphoteric compounds having pI values outside the pH region of the ampholytes applied in the capillary with high precision. The high resolution power of this technique was proven with the... (More)
- Substituted aminomethylphenol dyes, low-molecular-mass isoelectric point (pI ) markers and hemoglobin samples from normal individuals and diabetic patients were used to test a new set-up of capillary isoelectric focusing (cIEF) in uncoated capillaries. In previous cIEF methods, a mixture of sample components and carrier ampholytes was applied in the capillary and analyzed. In the new set-up a fractionated injection protocol is used to apply a ‘sandwich’ ampholyte–sample–ampholyte plug in the capillary for analysis. This new set-up allows the separation of amphoteric compounds having pI values outside the pH region of the ampholytes applied in the capillary with high precision. The high resolution power of this technique was proven with the analysis of hemoglobin variants. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3516344
- author
- Kilár, Ferenc ; Végvári, Ákos LU and Mód, Andor
- publishing date
- 1998
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Isoelectric focusing, Sandwich injection, Injection methods, Haemoglobin, Dyes, Nitrophenols
- in
- Journal of Chromatography A
- volume
- 813
- issue
- 2
- pages
- 349 - 360
- publisher
- Elsevier
- external identifiers
-
- scopus:0344541992
- ISSN
- 0021-9673
- DOI
- 10.1016/S0021-9673(98)00377-X
- language
- English
- LU publication?
- no
- id
- 0934bc21-a74a-4ecb-b0bb-8cc975fecb47 (old id 3516344)
- date added to LUP
- 2016-04-04 09:44:20
- date last changed
- 2022-03-31 08:00:15
@article{0934bc21-a74a-4ecb-b0bb-8cc975fecb47, abstract = {{Substituted aminomethylphenol dyes, low-molecular-mass isoelectric point (pI ) markers and hemoglobin samples from normal individuals and diabetic patients were used to test a new set-up of capillary isoelectric focusing (cIEF) in uncoated capillaries. In previous cIEF methods, a mixture of sample components and carrier ampholytes was applied in the capillary and analyzed. In the new set-up a fractionated injection protocol is used to apply a ‘sandwich’ ampholyte–sample–ampholyte plug in the capillary for analysis. This new set-up allows the separation of amphoteric compounds having pI values outside the pH region of the ampholytes applied in the capillary with high precision. The high resolution power of this technique was proven with the analysis of hemoglobin variants.}}, author = {{Kilár, Ferenc and Végvári, Ákos and Mód, Andor}}, issn = {{0021-9673}}, keywords = {{Isoelectric focusing; Sandwich injection; Injection methods; Haemoglobin; Dyes; Nitrophenols}}, language = {{eng}}, number = {{2}}, pages = {{349--360}}, publisher = {{Elsevier}}, series = {{Journal of Chromatography A}}, title = {{New Set-up for Capillary Isoelectric Focusing in Uncoated Capillaries}}, url = {{http://dx.doi.org/10.1016/S0021-9673(98)00377-X}}, doi = {{10.1016/S0021-9673(98)00377-X}}, volume = {{813}}, year = {{1998}}, }