GSM 900 MHz exposure of Transgenic GFAP-Mice: A study of histopathology, Tumour induction and BBB leakage
Salford, Leif LU ; Brun, Arne LU and Persson, Bertil R LU
(2012)
In Acta Scientiarum Lundensia
2012-003.
p.1-17
- Abstract
- Abstract. In previous work we have studied the effects of electromagnetic fields upon
the promotion of growth of tumours from two different rat glioma cell lines, RG2 and N32,
inoculated in the brains of Fischer 344 rats. No promotions of tumour growth due to the
EMF could be demonstrated in large series of animals as compared to their matched
controls. In the present study we have utilized a newly generated "knock-out" mouse
model. By the use of gene targeting, Pekny et al. have created mice deficient for glial
fibrillary acidic protein (GFAP), which is an astrocyte-specific protein that forms
intermediate filaments in the cytoskeleton of astrocytes. The GFAP-deficient mice... (More) - Abstract. In previous work we have studied the effects of electromagnetic fields upon
the promotion of growth of tumours from two different rat glioma cell lines, RG2 and N32,
inoculated in the brains of Fischer 344 rats. No promotions of tumour growth due to the
EMF could be demonstrated in large series of animals as compared to their matched
controls. In the present study we have utilized a newly generated "knock-out" mouse
model. By the use of gene targeting, Pekny et al. have created mice deficient for glial
fibrillary acidic protein (GFAP), which is an astrocyte-specific protein that forms
intermediate filaments in the cytoskeleton of astrocytes. The GFAP-deficient mice utilized
by us develop spontaneous tumours, both lymphoblastic (non-Hodgkin) lymphomas and
Anaplastic rapidly growing tumours diagnosed as malignant Swannomas. The test system
consists of four TEM-cells. A GSM telephone is hooked up to a terminal program on a
computer that controls the power and repetition of pulses. The rats were exposed for about
8 hours during 43 days for a total time of 1143 hours.
In male GFAP-mice we found tumours in all 6 controls and in 4 out of 6 exposed. We
found, however, no tumours in FM controls and in seven exposed FM mice we fund only 1
tumour. Microwave exposure revealed no significant effect on tumour evolution in the
GFAP-mice.
In the CBA-mice microwave exposure gave no significant results of BBB-leakage. But
for the CBA-mice BBB-leakage after microwave exposure of CBA-mice was found
probable at a 99% significance-level. (Less)
Please use this url to cite or link to this publication:
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- author
- Salford, Leif
LU
; Brun, Arne
LU
and Persson, Bertil R
LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- 900 MHz. exposure, GFAP, Transgenic Mice, tumour induction, BBB leakage, GSM
- in
- Acta Scientiarum Lundensia
- volume
- 2012-003
- pages
- 1 - 17
- publisher
- Bertil RR Persson, Medical Radiation Physics, 22185 Lund, Sweden
- ISSN
- 1651-5013
- language
- English
- LU publication?
- yes
- additional info
- We wish to thank professor Milos Pekny, MD, PhD, at the Institute of Neuroscience and Physiology, University of Gothenburg for giving us the opportunity to use his GFAP mice for this study. We also thank BMA Catarina Blennow and BMA Sussanne Strömblad at the Rausing Laboratory for their excellent technical assistance in the exposure and blood-sampling of all the mice. References Cardis
- id
- a5a631de-0f6f-49d7-9e9a-692359f7cd39 (old id 3910692)
- date added to LUP
- 2016-04-01 13:53:30
- date last changed
- 2018-11-21 20:20:58
@article{a5a631de-0f6f-49d7-9e9a-692359f7cd39,
abstract = {{Abstract. In previous work we have studied the effects of electromagnetic fields upon<br/><br>
the promotion of growth of tumours from two different rat glioma cell lines, RG2 and N32,<br/><br>
inoculated in the brains of Fischer 344 rats. No promotions of tumour growth due to the<br/><br>
EMF could be demonstrated in large series of animals as compared to their matched<br/><br>
controls. In the present study we have utilized a newly generated "knock-out" mouse<br/><br>
model. By the use of gene targeting, Pekny et al. have created mice deficient for glial<br/><br>
fibrillary acidic protein (GFAP), which is an astrocyte-specific protein that forms<br/><br>
intermediate filaments in the cytoskeleton of astrocytes. The GFAP-deficient mice utilized<br/><br>
by us develop spontaneous tumours, both lymphoblastic (non-Hodgkin) lymphomas and<br/><br>
Anaplastic rapidly growing tumours diagnosed as malignant Swannomas. The test system<br/><br>
consists of four TEM-cells. A GSM telephone is hooked up to a terminal program on a<br/><br>
computer that controls the power and repetition of pulses. The rats were exposed for about<br/><br>
8 hours during 43 days for a total time of 1143 hours.<br/><br>
In male GFAP-mice we found tumours in all 6 controls and in 4 out of 6 exposed. We<br/><br>
found, however, no tumours in FM controls and in seven exposed FM mice we fund only 1<br/><br>
tumour. Microwave exposure revealed no significant effect on tumour evolution in the<br/><br>
GFAP-mice.<br/><br>
In the CBA-mice microwave exposure gave no significant results of BBB-leakage. But<br/><br>
for the CBA-mice BBB-leakage after microwave exposure of CBA-mice was found<br/><br>
probable at a 99% significance-level.}},
author = {{Salford, Leif and Brun, Arne and Persson, Bertil R}},
issn = {{1651-5013}},
keywords = {{900 MHz. exposure; GFAP; Transgenic Mice; tumour induction; BBB leakage; GSM}},
language = {{eng}},
pages = {{1--17}},
publisher = {{Bertil RR Persson, Medical Radiation Physics, 22185 Lund, Sweden}},
series = {{Acta Scientiarum Lundensia}},
title = {{GSM 900 MHz exposure of Transgenic GFAP-Mice: A study of histopathology, Tumour induction and BBB leakage}},
volume = {{2012-003}},
year = {{2012}},
}