Cell harvesting by cross-flow microfiltration using a shear-enhanced module
(1996) In Biotechnology and Bioengineering 52(3). p.397-403- Abstract
- Protein, produced by a bacterial culture of recombinant Vibrio cholerae, was separated from cells in a fermentation broth by cross-flow microfiltration. A new, mechanically agitated (rotational) shear filter, the DMF(TM) filter from Pall, was used to perform the separation. Higher protein recovery and permeate flux than commonly obtained during cell harvesting were demonstrated using sixfold concentration followed by twofold diafiltration. The transmembrane pressure only increased by 10 kPa when the flux was kept constant at 150 L/m(2) h during both concentration and diafiltration. The protein transmission was about 100% initially, and over 90% at the end of the concentration process. The protein transmission during the diafiltration was... (More)
- Protein, produced by a bacterial culture of recombinant Vibrio cholerae, was separated from cells in a fermentation broth by cross-flow microfiltration. A new, mechanically agitated (rotational) shear filter, the DMF(TM) filter from Pall, was used to perform the separation. Higher protein recovery and permeate flux than commonly obtained during cell harvesting were demonstrated using sixfold concentration followed by twofold diafiltration. The transmembrane pressure only increased by 10 kPa when the flux was kept constant at 150 L/m(2) h during both concentration and diafiltration. The protein transmission was about 100% initially, and over 90% at the end of the concentration process. The protein transmission during the diafiltration was over 80%. The total recovery of protein was 97%. When using an enzymatic cleaning agent, no significant pure water flux decrease was detected during the course of the experiments. (C) 1996 John Wiley & Sons, Inc. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3915742
- author
- Frenander, U and Jönsson, Ann-Sofi LU
- organization
- publishing date
- 1996
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- cell harvesting, protein separation, Vibrio cholerae, cross-flow, microfiltration, shear-enhanced module
- in
- Biotechnology and Bioengineering
- volume
- 52
- issue
- 3
- pages
- 397 - 403
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:A1996VM10500005
- scopus:0030571269
- ISSN
- 1097-0290
- DOI
- 10.1002/(SICI)1097-0290(19961105)52:3<397::AID-BIT5>3.3.CO;2-1
- language
- English
- LU publication?
- yes
- id
- ae5ec9d8-fe45-4435-bc53-10d6e39ac887 (old id 3915742)
- date added to LUP
- 2016-04-01 12:19:32
- date last changed
- 2023-11-11 21:01:42
@article{ae5ec9d8-fe45-4435-bc53-10d6e39ac887, abstract = {{Protein, produced by a bacterial culture of recombinant Vibrio cholerae, was separated from cells in a fermentation broth by cross-flow microfiltration. A new, mechanically agitated (rotational) shear filter, the DMF(TM) filter from Pall, was used to perform the separation. Higher protein recovery and permeate flux than commonly obtained during cell harvesting were demonstrated using sixfold concentration followed by twofold diafiltration. The transmembrane pressure only increased by 10 kPa when the flux was kept constant at 150 L/m(2) h during both concentration and diafiltration. The protein transmission was about 100% initially, and over 90% at the end of the concentration process. The protein transmission during the diafiltration was over 80%. The total recovery of protein was 97%. When using an enzymatic cleaning agent, no significant pure water flux decrease was detected during the course of the experiments. (C) 1996 John Wiley & Sons, Inc.}}, author = {{Frenander, U and Jönsson, Ann-Sofi}}, issn = {{1097-0290}}, keywords = {{cell harvesting; protein separation; Vibrio cholerae; cross-flow; microfiltration; shear-enhanced module}}, language = {{eng}}, number = {{3}}, pages = {{397--403}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Biotechnology and Bioengineering}}, title = {{Cell harvesting by cross-flow microfiltration using a shear-enhanced module}}, url = {{http://dx.doi.org/10.1002/(SICI)1097-0290(19961105)52:3<397::AID-BIT5>3.3.CO;2-1}}, doi = {{10.1002/(SICI)1097-0290(19961105)52:3<397::AID-BIT5>3.3.CO;2-1}}, volume = {{52}}, year = {{1996}}, }