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Functional analysis of the mouse J chain

Erlandsson, Lena LU (1999)
Abstract
We have in this study analyzed the function of the murine Joining chain (J chain) in vivo. J chain is a polypeptide found in sera and secretions in complex with pentameric IgM and dimeric IgA. J chain is expressed in terminally differentiated B lymphocytes and needed for the transport of dimeric IgA and pentameric IgM over mucosal surfaces via the poly Ig receptor. A mouse deficient for J chain was generated and it was shown that J chain in vivo seems to be necessary for efficient assembly and secretion of polymeric IgM. Secondly, in the absence of J chain, antigen-specific IgA immunity in the lamina propria of the mucosa cannot mediate protection on the mucosal surface of the intestinal lumen; thereby demonstrating in vivo a direct... (More)
We have in this study analyzed the function of the murine Joining chain (J chain) in vivo. J chain is a polypeptide found in sera and secretions in complex with pentameric IgM and dimeric IgA. J chain is expressed in terminally differentiated B lymphocytes and needed for the transport of dimeric IgA and pentameric IgM over mucosal surfaces via the poly Ig receptor. A mouse deficient for J chain was generated and it was shown that J chain in vivo seems to be necessary for efficient assembly and secretion of polymeric IgM. Secondly, in the absence of J chain, antigen-specific IgA immunity in the lamina propria of the mucosa cannot mediate protection on the mucosal surface of the intestinal lumen; thereby demonstrating in vivo a direct relationship between mucosal transport of secretory IgA and intestinal immune protection. A second mouse was generated where the Diphtheria toxin A gene was introduced into the J chain locus, thereby ablating J chain expressing cells. Evidence was found for the existence of two separate plasma cell populations distinguishable by the absence or the presence of J chain. The dogma that in mice the J chain is expressed in all plasma cells irrespective of isotype has been challenged. (Less)
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author
supervisor
opponent
  • Dr Sideras, Paschalis, Umeå University, Sweden
organization
publishing date
type
Thesis
publication status
published
subject
keywords
Immunology, diphtheria toxin A, plasma cell, mucosa, J chain, knock-out, serology, transplantation, Immunologi, serologi
pages
140 pages
publisher
Immunology Unit, Sölvegatan 21, 223 62 Lund, Sweden
defense location
Sölvegatan 21, Lund
defense date
1999-05-05 10:15:00
external identifiers
  • other:ISRN: LUMEDW/MECM--99/1026--SE
ISBN
91-628-3544-0
language
English
LU publication?
yes
id
396d3d5f-8d01-4a2e-8303-42bd168b2cab (old id 39490)
date added to LUP
2016-04-04 10:41:39
date last changed
2018-11-21 21:00:15
@phdthesis{396d3d5f-8d01-4a2e-8303-42bd168b2cab,
  abstract     = {{We have in this study analyzed the function of the murine Joining chain (J chain) in vivo. J chain is a polypeptide found in sera and secretions in complex with pentameric IgM and dimeric IgA. J chain is expressed in terminally differentiated B lymphocytes and needed for the transport of dimeric IgA and pentameric IgM over mucosal surfaces via the poly Ig receptor. A mouse deficient for J chain was generated and it was shown that J chain in vivo seems to be necessary for efficient assembly and secretion of polymeric IgM. Secondly, in the absence of J chain, antigen-specific IgA immunity in the lamina propria of the mucosa cannot mediate protection on the mucosal surface of the intestinal lumen; thereby demonstrating in vivo a direct relationship between mucosal transport of secretory IgA and intestinal immune protection. A second mouse was generated where the Diphtheria toxin A gene was introduced into the J chain locus, thereby ablating J chain expressing cells. Evidence was found for the existence of two separate plasma cell populations distinguishable by the absence or the presence of J chain. The dogma that in mice the J chain is expressed in all plasma cells irrespective of isotype has been challenged.}},
  author       = {{Erlandsson, Lena}},
  isbn         = {{91-628-3544-0}},
  keywords     = {{Immunology; diphtheria toxin A; plasma cell; mucosa; J chain; knock-out; serology; transplantation; Immunologi; serologi}},
  language     = {{eng}},
  publisher    = {{Immunology Unit, Sölvegatan 21, 223 62 Lund, Sweden}},
  school       = {{Lund University}},
  title        = {{Functional analysis of the mouse J chain}},
  year         = {{1999}},
}