A cDNA coding for human sex hormone binding globulin. Homology to vitamin K-dependent protein S
(1987) In FEBS Letters 220(1). p.35-129- Abstract
- Affinity purified antibodies to human sex hormone binding globulin (SHBG) were used in screening a human liver cDNA library, constructed in the expression vector lambda gt11. One clone, identified as producing recombinant SHBG, carried a cDNA insert of 1.1 kb. The nucleotide sequence of the insert had an open reading frame coding for 356 amino acid residues. The coding sequence was followed by a short 3'-region of 19 non-translated nucleotides and a poly(A) tail. Confirmation that the cDNA clone represented human SHBG was obtained by the finding of a complete agreement in amino acid sequence with several peptide fragments generated from purified SHBG by proteolytic cleavage. The primary structure of SHBG shows a considerable homology to... (More)
- Affinity purified antibodies to human sex hormone binding globulin (SHBG) were used in screening a human liver cDNA library, constructed in the expression vector lambda gt11. One clone, identified as producing recombinant SHBG, carried a cDNA insert of 1.1 kb. The nucleotide sequence of the insert had an open reading frame coding for 356 amino acid residues. The coding sequence was followed by a short 3'-region of 19 non-translated nucleotides and a poly(A) tail. Confirmation that the cDNA clone represented human SHBG was obtained by the finding of a complete agreement in amino acid sequence with several peptide fragments generated from purified SHBG by proteolytic cleavage. The primary structure of SHBG shows a considerable homology to that of protein S, a vitamin K-dependent protein with functions in the coagulation system. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3965145
- author
- Gershagen, S. ; Fernlund, Per LU and Lundwall, Åke LU
- organization
- publishing date
- 1987
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Protein S, Peptide Fragments/analysis, Liver/analysis, Humans, Glycoproteins/analysis/*genetics, DNA/*analysis, Comparative Study, Cattle, *Base Sequence, Amino Acid Sequence, Animals, Research Support, Non-U.S. Gov't, *Sequence Homology, Nucleic Acid, Sex Hormone-Binding Globulin/analysis/*genetics
- in
- FEBS Letters
- volume
- 220
- issue
- 1
- pages
- 35 - 129
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:0023273737
- ISSN
- 1873-3468
- language
- English
- LU publication?
- yes
- additional info
- 1
- id
- e85083db-805b-4366-bba0-07c67b230da7 (old id 3965145)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2956126
- date added to LUP
- 2016-04-04 13:50:12
- date last changed
- 2021-08-29 05:33:01
@article{e85083db-805b-4366-bba0-07c67b230da7, abstract = {{Affinity purified antibodies to human sex hormone binding globulin (SHBG) were used in screening a human liver cDNA library, constructed in the expression vector lambda gt11. One clone, identified as producing recombinant SHBG, carried a cDNA insert of 1.1 kb. The nucleotide sequence of the insert had an open reading frame coding for 356 amino acid residues. The coding sequence was followed by a short 3'-region of 19 non-translated nucleotides and a poly(A) tail. Confirmation that the cDNA clone represented human SHBG was obtained by the finding of a complete agreement in amino acid sequence with several peptide fragments generated from purified SHBG by proteolytic cleavage. The primary structure of SHBG shows a considerable homology to that of protein S, a vitamin K-dependent protein with functions in the coagulation system.}}, author = {{Gershagen, S. and Fernlund, Per and Lundwall, Åke}}, issn = {{1873-3468}}, keywords = {{Protein S; Peptide Fragments/analysis; Liver/analysis; Humans; Glycoproteins/analysis/*genetics; DNA/*analysis; Comparative Study; Cattle; *Base Sequence; Amino Acid Sequence; Animals; Research Support; Non-U.S. Gov't; *Sequence Homology; Nucleic Acid; Sex Hormone-Binding Globulin/analysis/*genetics}}, language = {{eng}}, number = {{1}}, pages = {{35--129}}, publisher = {{Wiley-Blackwell}}, series = {{FEBS Letters}}, title = {{A cDNA coding for human sex hormone binding globulin. Homology to vitamin K-dependent protein S}}, url = {{http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2956126}}, volume = {{220}}, year = {{1987}}, }