Chemical characterization of the predominant proteins secreted by mouse seminal vesicles
(1997) In Eur J Biochem 249(1). p.39-44- Abstract
- Mouse seminal vesicles secrete four major protein components with estimated molecular masses of 95, 38, 17, and 16 kDa. Amino acid sequencing revealed that the 95-kDa component represents a protein with an unknown structure, while the 38-kDa component was identified as semenoclotin, the 17-kDa component as seminal-vesicle-secreted protein IV, and the 16-kDa component as seminal-vesicle-secreted protein V. Semenoclotin and the 95-kDa component were readily cross-linked by transglutaminase, suggesting that the two proteins are involved in the formation of the mouse copulatory plug. Treatment of mouse seminal vesicle fluid with human prostate-specific antigen rapidly degraded semenoclotin, indicating a structural resemblance of this protein... (More)
- Mouse seminal vesicles secrete four major protein components with estimated molecular masses of 95, 38, 17, and 16 kDa. Amino acid sequencing revealed that the 95-kDa component represents a protein with an unknown structure, while the 38-kDa component was identified as semenoclotin, the 17-kDa component as seminal-vesicle-secreted protein IV, and the 16-kDa component as seminal-vesicle-secreted protein V. Semenoclotin and the 95-kDa component were readily cross-linked by transglutaminase, suggesting that the two proteins are involved in the formation of the mouse copulatory plug. Treatment of mouse seminal vesicle fluid with human prostate-specific antigen rapidly degraded semenoclotin, indicating a structural resemblance of this protein to human semenogelins, despite the vast difference in primary structure. As previously reported for other seminal-vesicle-secreted proteins, the semenoclotin transcripts are shown to be under androgen control. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3965273
- author
- Lundwall, Åke LU ; Peter, A. ; Lövgren, J. ; Lilja, Hans LU and Malm, J.
- organization
- publishing date
- 1997
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- RNA, Proteins/*chemistry/genetics/*secretion, Post-Translational, Protein Processing, Molecular Weight, Inbred BALB C, Mice, Male, Humans, Gonadal Steroid Hormones/chemistry/genetics, Cross-Linking Reagents, Amino Acid Sequence, Animals, Messenger/genetics/metabolism, Research Support, Non-U.S. Gov't, *Seminal Vesicle Secretory Proteins, Seminal Vesicles/chemistry/drug effects/*secretion, Testosterone/pharmacology, Transglutaminases
- in
- Eur J Biochem
- volume
- 249
- issue
- 1
- pages
- 39 - 44
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:0030819922
- language
- English
- LU publication?
- yes
- additional info
- 1
- id
- 2fd412b7-59a1-4eda-8896-5f4707fd633e (old id 3965273)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9363751
- date added to LUP
- 2016-04-04 13:59:34
- date last changed
- 2022-01-30 01:18:01
@article{2fd412b7-59a1-4eda-8896-5f4707fd633e, abstract = {{Mouse seminal vesicles secrete four major protein components with estimated molecular masses of 95, 38, 17, and 16 kDa. Amino acid sequencing revealed that the 95-kDa component represents a protein with an unknown structure, while the 38-kDa component was identified as semenoclotin, the 17-kDa component as seminal-vesicle-secreted protein IV, and the 16-kDa component as seminal-vesicle-secreted protein V. Semenoclotin and the 95-kDa component were readily cross-linked by transglutaminase, suggesting that the two proteins are involved in the formation of the mouse copulatory plug. Treatment of mouse seminal vesicle fluid with human prostate-specific antigen rapidly degraded semenoclotin, indicating a structural resemblance of this protein to human semenogelins, despite the vast difference in primary structure. As previously reported for other seminal-vesicle-secreted proteins, the semenoclotin transcripts are shown to be under androgen control.}}, author = {{Lundwall, Åke and Peter, A. and Lövgren, J. and Lilja, Hans and Malm, J.}}, keywords = {{RNA; Proteins/*chemistry/genetics/*secretion; Post-Translational; Protein Processing; Molecular Weight; Inbred BALB C; Mice; Male; Humans; Gonadal Steroid Hormones/chemistry/genetics; Cross-Linking Reagents; Amino Acid Sequence; Animals; Messenger/genetics/metabolism; Research Support; Non-U.S. Gov't; *Seminal Vesicle Secretory Proteins; Seminal Vesicles/chemistry/drug effects/*secretion; Testosterone/pharmacology; Transglutaminases}}, language = {{eng}}, number = {{1}}, pages = {{39--44}}, publisher = {{Wiley-Blackwell}}, series = {{Eur J Biochem}}, title = {{Chemical characterization of the predominant proteins secreted by mouse seminal vesicles}}, url = {{http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9363751}}, volume = {{249}}, year = {{1997}}, }