Experimental Models to Study Drug Distributions in Tissue Using MALDI Mass Spectrometry
(2013) In Journal of Proteome Research 12(12). p.5626-5633- Abstract
- Requirements for patient safety and improved efficacy are steadily increasing in modern healthcare and are key drivers in modern drug development. New drug characterization assays are central in providing evidence of the specificity and selectivity of drugs. Meeting this need, matrix-assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) is used to study drug localization within micro-environmental tissue compartments. Thin sections of human lung tumor and rat xenograft tissues were exposed to pharmaceutical drugs by either spotting or submerging. These drugs, the epidermal growth factor receptor antagonists, erlotinib (Tarceva™) and gefitinib (Iressa™), and the acetylcholine receptor antagonist, tiotropium were... (More)
- Requirements for patient safety and improved efficacy are steadily increasing in modern healthcare and are key drivers in modern drug development. New drug characterization assays are central in providing evidence of the specificity and selectivity of drugs. Meeting this need, matrix-assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) is used to study drug localization within micro-environmental tissue compartments. Thin sections of human lung tumor and rat xenograft tissues were exposed to pharmaceutical drugs by either spotting or submerging. These drugs, the epidermal growth factor receptor antagonists, erlotinib (Tarceva™) and gefitinib (Iressa™), and the acetylcholine receptor antagonist, tiotropium were characterized by microenvironment localization. Intact tissue blocks were also immersed in drug solution followed by sectioning. MALDI-MSI was then performed using a Thermo MALDI LTQ Orbitrap XL instrument to localize drug distribution patterns. We propose three MALDI-MSI models measuring drug disposition that have been used to map the selected compounds within tissue compartments of tumors isolated from lung cancer patients. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4193466
- author
- Végvári, Ákos LU ; Fehniger, Thomas E. ; Rezeli, Melinda LU ; Laurell, Thomas LU ; Döme, Balazs ; Jansson, Bo ; Welinder, Charlotte LU and Marko-Varga, György LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Lung Cancer, Adenocarcinoma, MALDI-Mass Spectrometry Imaging, Erlotinib, Gefitinib, Tiotropium
- in
- Journal of Proteome Research
- volume
- 12
- issue
- 12
- pages
- 5626 - 5633
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000328231300024
- scopus:84890104949
- pmid:24134601
- ISSN
- 1535-3893
- DOI
- 10.1021/pr400581b
- language
- English
- LU publication?
- yes
- id
- 14b7a42c-fb6e-45df-96e2-49f8feb89ab9 (old id 4193466)
- date added to LUP
- 2016-04-01 10:19:30
- date last changed
- 2023-08-30 23:48:23
@article{14b7a42c-fb6e-45df-96e2-49f8feb89ab9, abstract = {{Requirements for patient safety and improved efficacy are steadily increasing in modern healthcare and are key drivers in modern drug development. New drug characterization assays are central in providing evidence of the specificity and selectivity of drugs. Meeting this need, matrix-assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) is used to study drug localization within micro-environmental tissue compartments. Thin sections of human lung tumor and rat xenograft tissues were exposed to pharmaceutical drugs by either spotting or submerging. These drugs, the epidermal growth factor receptor antagonists, erlotinib (Tarceva™) and gefitinib (Iressa™), and the acetylcholine receptor antagonist, tiotropium were characterized by microenvironment localization. Intact tissue blocks were also immersed in drug solution followed by sectioning. MALDI-MSI was then performed using a Thermo MALDI LTQ Orbitrap XL instrument to localize drug distribution patterns. We propose three MALDI-MSI models measuring drug disposition that have been used to map the selected compounds within tissue compartments of tumors isolated from lung cancer patients.}}, author = {{Végvári, Ákos and Fehniger, Thomas E. and Rezeli, Melinda and Laurell, Thomas and Döme, Balazs and Jansson, Bo and Welinder, Charlotte and Marko-Varga, György}}, issn = {{1535-3893}}, keywords = {{Lung Cancer; Adenocarcinoma; MALDI-Mass Spectrometry Imaging; Erlotinib; Gefitinib; Tiotropium}}, language = {{eng}}, number = {{12}}, pages = {{5626--5633}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Journal of Proteome Research}}, title = {{Experimental Models to Study Drug Distributions in Tissue Using MALDI Mass Spectrometry}}, url = {{http://dx.doi.org/10.1021/pr400581b}}, doi = {{10.1021/pr400581b}}, volume = {{12}}, year = {{2013}}, }