Protein Expression Profiling of Formalin-Fixed Paraffin-Embedded Tissue Using Recombinant Antibody Microarrays
(2013) In Journal of Proteome Research 12(12). p.5943-5953- Abstract
- Proteomics, the large-scale analysis of proteins, is a rapidly evolving field with an increasing number of key clinical applications, such as diagnosis, prognosis, and classification. In order to generate complete protein expression profiles, or protein atlases, any crude sample format must be addressable in a rapid, multiplex, and sensitive manner. A common and clinically central sample format, formalin-fixed, paraffin-embedded (FFPE) tissue material, holds great potential as a source for disease-associated biomarker signatures. However, despite major efforts, extraction and subsequent profiling of proteins from FFPE tissue has proven to be challenging. In this proof-of-concept study, we have demonstrated for the first time that proteins... (More)
- Proteomics, the large-scale analysis of proteins, is a rapidly evolving field with an increasing number of key clinical applications, such as diagnosis, prognosis, and classification. In order to generate complete protein expression profiles, or protein atlases, any crude sample format must be addressable in a rapid, multiplex, and sensitive manner. A common and clinically central sample format, formalin-fixed, paraffin-embedded (FFPE) tissue material, holds great potential as a source for disease-associated biomarker signatures. However, despite major efforts, extraction and subsequent profiling of proteins from FFPE tissue has proven to be challenging. In this proof-of-concept study, we have demonstrated for the first time that proteins could be extracted, labeled, and subsequently profiled in a multiplex, sensitive, and reproducible manner using recombinant scFv antibody microarrays. Thus, we have added FFPE samples to the list of sample formats available for high-throughput analysis by affinity proteomics, paving the way for the next generation of biomarker-driven discovery projects. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4272433
- author
- Pauly, Frida LU ; Dexlin Mellby, Linda LU ; Ek, Sara LU ; Ohlin, Mats LU ; Olsson, Niclas LU ; Jirström, Karin LU ; Dictor, Michael LU ; Schoenmakers, Saskia ; Borrebaeck, Carl LU and Wingren, Christer LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- antibody microarrays, FFPE, protein expression profiling
- in
- Journal of Proteome Research
- volume
- 12
- issue
- 12
- pages
- 5943 - 5953
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000328231300052
- scopus:84890055112
- pmid:24063262
- ISSN
- 1535-3893
- DOI
- 10.1021/pr4003245
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pathology, (Lund) (013030000), Department of Immunotechnology (011029300)
- id
- f909ae9f-5b1c-4a0a-9377-733f7c103d5d (old id 4272433)
- date added to LUP
- 2016-04-01 10:42:03
- date last changed
- 2024-01-06 22:57:06
@article{f909ae9f-5b1c-4a0a-9377-733f7c103d5d, abstract = {{Proteomics, the large-scale analysis of proteins, is a rapidly evolving field with an increasing number of key clinical applications, such as diagnosis, prognosis, and classification. In order to generate complete protein expression profiles, or protein atlases, any crude sample format must be addressable in a rapid, multiplex, and sensitive manner. A common and clinically central sample format, formalin-fixed, paraffin-embedded (FFPE) tissue material, holds great potential as a source for disease-associated biomarker signatures. However, despite major efforts, extraction and subsequent profiling of proteins from FFPE tissue has proven to be challenging. In this proof-of-concept study, we have demonstrated for the first time that proteins could be extracted, labeled, and subsequently profiled in a multiplex, sensitive, and reproducible manner using recombinant scFv antibody microarrays. Thus, we have added FFPE samples to the list of sample formats available for high-throughput analysis by affinity proteomics, paving the way for the next generation of biomarker-driven discovery projects.}}, author = {{Pauly, Frida and Dexlin Mellby, Linda and Ek, Sara and Ohlin, Mats and Olsson, Niclas and Jirström, Karin and Dictor, Michael and Schoenmakers, Saskia and Borrebaeck, Carl and Wingren, Christer}}, issn = {{1535-3893}}, keywords = {{antibody microarrays; FFPE; protein expression profiling}}, language = {{eng}}, number = {{12}}, pages = {{5943--5953}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Journal of Proteome Research}}, title = {{Protein Expression Profiling of Formalin-Fixed Paraffin-Embedded Tissue Using Recombinant Antibody Microarrays}}, url = {{http://dx.doi.org/10.1021/pr4003245}}, doi = {{10.1021/pr4003245}}, volume = {{12}}, year = {{2013}}, }