Dual Application of Cryogel as Solid Support in Peptide Synthesis and Subsequent Protein-Capture
(2013) In Journal of Applied Polymer Science 130(6). p.4383-4391- Abstract
- The use of a cryogel in a combined application as a solid support for automated synthesis of a peptide ligand followed by affinity chromatography of a target protein is evaluated. The advantage, of synthesizing the ligand directly on the cryogel, is the circumvention of the standard process of synthesizing a peptide on a solid support, followed by cleavage, purification, analysis, and finally immobilization on the cryogel. To demonstrate the application, a peptide affinity ligand is synthesized directly on a cryogel with a yield of 28.4 mol g(-1) dry polymer and purity of 45% of crude product. The affinity capture of an antipeptide antibody reveals a specific binding capacity of 0.86 mg g(-1) dry polymer. To further elucidate the general... (More)
- The use of a cryogel in a combined application as a solid support for automated synthesis of a peptide ligand followed by affinity chromatography of a target protein is evaluated. The advantage, of synthesizing the ligand directly on the cryogel, is the circumvention of the standard process of synthesizing a peptide on a solid support, followed by cleavage, purification, analysis, and finally immobilization on the cryogel. To demonstrate the application, a peptide affinity ligand is synthesized directly on a cryogel with a yield of 28.4 mol g(-1) dry polymer and purity of 45% of crude product. The affinity capture of an antipeptide antibody reveals a specific binding capacity of 0.86 mg g(-1) dry polymer. To further elucidate the general availability of a peptide ligand to a macromolecular interaction, a trypsin substrate is synthesized on a cryogel. Trypsin cleavage of immobilized substrate is determined to 1.5 mol g(-1) dry polymer. (c) 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 130: 4383-4391, 2013 (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4327132
- author
- Jespersen, Gry LU ; Nielsen, Anders Laerke ; Matthiesen, Finn ; Andersen, Henrik Sune and Kirsebom, Harald LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- gels, porous materials, proteins, separation techniques
- in
- Journal of Applied Polymer Science
- volume
- 130
- issue
- 6
- pages
- 4383 - 4391
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000329153300063
- scopus:84885018912
- ISSN
- 1097-4628
- DOI
- 10.1002/app.39727
- language
- English
- LU publication?
- yes
- id
- 6ea395b4-c0a6-4d45-aa7e-9e3b1e4c139b (old id 4327132)
- date added to LUP
- 2016-04-01 10:33:32
- date last changed
- 2022-01-26 00:22:40
@article{6ea395b4-c0a6-4d45-aa7e-9e3b1e4c139b, abstract = {{The use of a cryogel in a combined application as a solid support for automated synthesis of a peptide ligand followed by affinity chromatography of a target protein is evaluated. The advantage, of synthesizing the ligand directly on the cryogel, is the circumvention of the standard process of synthesizing a peptide on a solid support, followed by cleavage, purification, analysis, and finally immobilization on the cryogel. To demonstrate the application, a peptide affinity ligand is synthesized directly on a cryogel with a yield of 28.4 mol g(-1) dry polymer and purity of 45% of crude product. The affinity capture of an antipeptide antibody reveals a specific binding capacity of 0.86 mg g(-1) dry polymer. To further elucidate the general availability of a peptide ligand to a macromolecular interaction, a trypsin substrate is synthesized on a cryogel. Trypsin cleavage of immobilized substrate is determined to 1.5 mol g(-1) dry polymer. (c) 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 130: 4383-4391, 2013}}, author = {{Jespersen, Gry and Nielsen, Anders Laerke and Matthiesen, Finn and Andersen, Henrik Sune and Kirsebom, Harald}}, issn = {{1097-4628}}, keywords = {{gels; porous materials; proteins; separation techniques}}, language = {{eng}}, number = {{6}}, pages = {{4383--4391}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Journal of Applied Polymer Science}}, title = {{Dual Application of Cryogel as Solid Support in Peptide Synthesis and Subsequent Protein-Capture}}, url = {{http://dx.doi.org/10.1002/app.39727}}, doi = {{10.1002/app.39727}}, volume = {{130}}, year = {{2013}}, }