Multiplexing of miniaturized planar antibody arrays for serum protein profiling - a biomarker discovery in SLE nephritis.
(2014) In Lab on a Chip 14(11). p.1931-1942- Abstract
- In the quest to decipher disease-associated biomarkers, miniaturized and multiplexed antibody arrays may play a central role in generating protein expression profiles, or protein maps, of crude serum samples. In this conceptual study, we explored a novel, 4-times larger pen design, enabling us to, in a unique manner, simultaneously print 48 different reagents (antibodies) as individual 78.5 μm(2) (10 μm in diameter) sized spots at a density of 38 000 spots cm(-2) using dip-pen nanolithography technology. The antibody array set-up was interfaced with a high-resolution fluorescent-based scanner for sensitive sensing. The performance and applicability of this novel 48-plex recombinant antibody array platform design was demonstrated in a first... (More)
- In the quest to decipher disease-associated biomarkers, miniaturized and multiplexed antibody arrays may play a central role in generating protein expression profiles, or protein maps, of crude serum samples. In this conceptual study, we explored a novel, 4-times larger pen design, enabling us to, in a unique manner, simultaneously print 48 different reagents (antibodies) as individual 78.5 μm(2) (10 μm in diameter) sized spots at a density of 38 000 spots cm(-2) using dip-pen nanolithography technology. The antibody array set-up was interfaced with a high-resolution fluorescent-based scanner for sensitive sensing. The performance and applicability of this novel 48-plex recombinant antibody array platform design was demonstrated in a first clinical application targeting SLE nephritis, a severe chronic autoimmune connective tissue disorder, as the model disease. To this end, crude, directly biotinylated serum samples were targeted. The results showed that the miniaturized and multiplexed array platform displayed adequate performance, and that SLE-associated serum biomarker panels reflecting the disease process could be deciphered, outlining the use of miniaturized antibody arrays for disease proteomics and biomarker discovery. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4429505
- author
- Petersson, Linn ; Dexlin-Mellby, Linda ; Bengtsson, Anders LU ; Sturfelt, Gunnar LU ; Borrebaeck, Carl A K and Wingren, Christer
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Lab on a Chip
- volume
- 14
- issue
- 11
- pages
- 1931 - 1942
- publisher
- Royal Society of Chemistry
- external identifiers
-
- wos:000335925400019
- pmid:24763547
- scopus:84899873765
- ISSN
- 1473-0189
- DOI
- 10.1039/c3lc51420j
- language
- English
- LU publication?
- yes
- id
- cd455539-64fe-47cb-a31c-e70d4fb289cd (old id 4429505)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/24763547?dopt=Abstract
- date added to LUP
- 2016-04-01 10:29:17
- date last changed
- 2022-02-10 02:39:44
@article{cd455539-64fe-47cb-a31c-e70d4fb289cd,
abstract = {{In the quest to decipher disease-associated biomarkers, miniaturized and multiplexed antibody arrays may play a central role in generating protein expression profiles, or protein maps, of crude serum samples. In this conceptual study, we explored a novel, 4-times larger pen design, enabling us to, in a unique manner, simultaneously print 48 different reagents (antibodies) as individual 78.5 μm(2) (10 μm in diameter) sized spots at a density of 38 000 spots cm(-2) using dip-pen nanolithography technology. The antibody array set-up was interfaced with a high-resolution fluorescent-based scanner for sensitive sensing. The performance and applicability of this novel 48-plex recombinant antibody array platform design was demonstrated in a first clinical application targeting SLE nephritis, a severe chronic autoimmune connective tissue disorder, as the model disease. To this end, crude, directly biotinylated serum samples were targeted. The results showed that the miniaturized and multiplexed array platform displayed adequate performance, and that SLE-associated serum biomarker panels reflecting the disease process could be deciphered, outlining the use of miniaturized antibody arrays for disease proteomics and biomarker discovery.}},
author = {{Petersson, Linn and Dexlin-Mellby, Linda and Bengtsson, Anders and Sturfelt, Gunnar and Borrebaeck, Carl A K and Wingren, Christer}},
issn = {{1473-0189}},
language = {{eng}},
number = {{11}},
pages = {{1931--1942}},
publisher = {{Royal Society of Chemistry}},
series = {{Lab on a Chip}},
title = {{Multiplexing of miniaturized planar antibody arrays for serum protein profiling - a biomarker discovery in SLE nephritis.}},
url = {{http://dx.doi.org/10.1039/c3lc51420j}},
doi = {{10.1039/c3lc51420j}},
volume = {{14}},
year = {{2014}},
}