Boosting of HIV-1 Neutralizing Antibody Responses by a Distally Related Retroviral Envelope Protein.
(2014) In Journal of Immunology 192(12). p.5802-5812- Abstract
- Our knowledge of the binding sites for neutralizing Abs (NAb) that recognize a broad range of HIV-1 strains (bNAb) has substantially increased in recent years. However, gaps remain in our understanding of how to focus B cell responses to vulnerable conserved sites within the HIV-1 envelope glycoprotein (Env). In this article, we report an immunization strategy composed of a trivalent HIV-1 (clade B envs) DNA prime, followed by a SIVmac239 gp140 Env protein boost that aimed to focus the immune response to structurally conserved parts of the HIV-1 and simian immunodeficiency virus (SIV) Envs. Heterologous NAb titers, primarily to tier 1 HIV-1 isolates, elicited during the trivalent HIV-1 env prime, were significantly increased by the... (More)
- Our knowledge of the binding sites for neutralizing Abs (NAb) that recognize a broad range of HIV-1 strains (bNAb) has substantially increased in recent years. However, gaps remain in our understanding of how to focus B cell responses to vulnerable conserved sites within the HIV-1 envelope glycoprotein (Env). In this article, we report an immunization strategy composed of a trivalent HIV-1 (clade B envs) DNA prime, followed by a SIVmac239 gp140 Env protein boost that aimed to focus the immune response to structurally conserved parts of the HIV-1 and simian immunodeficiency virus (SIV) Envs. Heterologous NAb titers, primarily to tier 1 HIV-1 isolates, elicited during the trivalent HIV-1 env prime, were significantly increased by the SIVmac239 gp140 protein boost in rabbits. Epitope mapping of Ab-binding reactivity revealed preferential recognition of the C1, C2, V2, V3, and V5 regions. These results provide a proof of concept that a distally related retroviral SIV Env protein boost can increase pre-existing NAb responses against HIV-1. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4455138
- author
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Immunology
- volume
- 192
- issue
- 12
- pages
- 5802 - 5812
- publisher
- American Association of Immunologists
- external identifiers
-
- pmid:24829409
- wos:000337172100040
- scopus:84902181476
- pmid:24829409
- ISSN
- 1550-6606
- DOI
- 10.4049/jimmunol.1301898
- language
- English
- LU publication?
- yes
- id
- 92238bc3-6e13-46fc-bf77-2ffff29e72b1 (old id 4455138)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/24829409?dopt=Abstract
- date added to LUP
- 2016-04-01 10:30:44
- date last changed
- 2022-02-02 18:30:22
@article{92238bc3-6e13-46fc-bf77-2ffff29e72b1, abstract = {{Our knowledge of the binding sites for neutralizing Abs (NAb) that recognize a broad range of HIV-1 strains (bNAb) has substantially increased in recent years. However, gaps remain in our understanding of how to focus B cell responses to vulnerable conserved sites within the HIV-1 envelope glycoprotein (Env). In this article, we report an immunization strategy composed of a trivalent HIV-1 (clade B envs) DNA prime, followed by a SIVmac239 gp140 Env protein boost that aimed to focus the immune response to structurally conserved parts of the HIV-1 and simian immunodeficiency virus (SIV) Envs. Heterologous NAb titers, primarily to tier 1 HIV-1 isolates, elicited during the trivalent HIV-1 env prime, were significantly increased by the SIVmac239 gp140 protein boost in rabbits. Epitope mapping of Ab-binding reactivity revealed preferential recognition of the C1, C2, V2, V3, and V5 regions. These results provide a proof of concept that a distally related retroviral SIV Env protein boost can increase pre-existing NAb responses against HIV-1.}}, author = {{Uchtenhagen, Hannes and Schiffner, Torben and Bowles, Emma and Heyndrickx, Leo and LaBranche, Celia and Applequist, Steven E and Jansson, Marianne and De Silva, Thushan and Back, Jaap Willem and Achour, Adnane and Scarlatti, Gabriella and Fomsgaard, Anders and Montefiori, David and Stewart-Jones, Guillaume and Spetz, Anna-Lena}}, issn = {{1550-6606}}, language = {{eng}}, number = {{12}}, pages = {{5802--5812}}, publisher = {{American Association of Immunologists}}, series = {{Journal of Immunology}}, title = {{Boosting of HIV-1 Neutralizing Antibody Responses by a Distally Related Retroviral Envelope Protein.}}, url = {{http://dx.doi.org/10.4049/jimmunol.1301898}}, doi = {{10.4049/jimmunol.1301898}}, volume = {{192}}, year = {{2014}}, }