Applications of laser spectroscopy to analytical chemistry, environmental monitoring and medicine
(2002) In Lund Reports on Atomic Physics- Abstract
- In this thesis spectroscopic techniques have been used in the fields of analytical chemistry, environmental and medical applications. In analytical chemistry, laser induced fluorescence was used to image the separation of substances during capillary electrophoresis. Here, an optical fibre imaging technique facilitated a higher sensitivity compared with a lens imaging technique. The motion of the substances was also monitored. In a similar experiment, laser induced fluorescence was used to image substances that do not exhibit fluorescence. Levitated drops have also been used as a wall-free cuvette during the study of fat-cell behaviour and protein precipitation, where laser induced fluorescence detection was used in the first part and light... (More)
- In this thesis spectroscopic techniques have been used in the fields of analytical chemistry, environmental and medical applications. In analytical chemistry, laser induced fluorescence was used to image the separation of substances during capillary electrophoresis. Here, an optical fibre imaging technique facilitated a higher sensitivity compared with a lens imaging technique. The motion of the substances was also monitored. In a similar experiment, laser induced fluorescence was used to image substances that do not exhibit fluorescence. Levitated drops have also been used as a wall-free cuvette during the study of fat-cell behaviour and protein precipitation, where laser induced fluorescence detection was used in the first part and light scattering detection in the second part.
For environmental monitoring a lidar (light detection and ranging) system was used. Here, measurements have been performed at different monumental buildings to detect both biodeteriogen invasion and protective treatment. The system was also moved onto a ship for experiments in the Mediterranean Sea. Laser induced fluorescence was here used for monitoring of chlorophyll contents in the seawater together with distributed organic matter (DOM). With the use of depth resolved measurements, the water absorption was studied.
In medical applications, laser induced fluorescence techniques have been used for spectral imaging of human normal and tumour tissue. For the treatment of the tumours, photodynamic therapy was employed. Three to six optical fibres, connected to a diode laser, were used and inserted in the tumour tissue. A sensitising agent was utilised to demarcate tumour tissue by fluorescence, and also to induce cell death. (Less) - Abstract (Swedish)
- Popular Abstract in Swedish
Det ljus som vi vanligen ser, har spritts av objekten i vår omgivning. Ett annat ljusfenomen är fluorescens, som kan användas inom olika områden för analys av prover. Olika substanser ger skilda fluorescensprofiler. Om dessa inte helt överlappar varandra kan substanserna särskiljas. Områden, där ljusdiagnostik har använts inom ramen för detta arbete, är kemisk analys, miljömätteknik och cancerdiagnostik. Likaså har ljus använts för cancerbehandling.
Inom analytisk kemi används många olika detektionsmetoder. Här har i huvudsak laserinducerad fluorescens använts inom separationstekniken kapillärelektrofores. I denna teknik används mycket tunna rör (kapillärer) för separation av... (More) - Popular Abstract in Swedish
Det ljus som vi vanligen ser, har spritts av objekten i vår omgivning. Ett annat ljusfenomen är fluorescens, som kan användas inom olika områden för analys av prover. Olika substanser ger skilda fluorescensprofiler. Om dessa inte helt överlappar varandra kan substanserna särskiljas. Områden, där ljusdiagnostik har använts inom ramen för detta arbete, är kemisk analys, miljömätteknik och cancerdiagnostik. Likaså har ljus använts för cancerbehandling.
Inom analytisk kemi används många olika detektionsmetoder. Här har i huvudsak laserinducerad fluorescens använts inom separationstekniken kapillärelektrofores. I denna teknik används mycket tunna rör (kapillärer) för separation av substanser med hjälp av ett elektriskt fält. För detektion i dessa kapillärer behövs en känslig metod och fluorescens kan då användas. För effektiv ljusinsamling användes optiska fibrer vilket gav en högre känslighet med en faktor 10. En annan analysmetod inom analytisk kemi är att med ljus belysa svävande droppar innehållande substansen av intresse. Även här utnyttjas fluorescenstekniken för detektion. Ett exempel på ett sådant experiment är att undersöka fettceller med avseende på deras fettsyreproduktion. Vid en tredje typ av undersökning studerades vilka betingelser som behövs för att kristallisering av protein skall ske. Detta undersöktes med hjälp av ljusspridningsteknik.
Fluorescens användes också för undersökning av algbeväxning på historiska byggnader som Lunds domkyrka och Parmas katedral. För att kunna mäta på alla svåråtkomliga ställen användes en metod för mätning på långt avstånd. Här skickas en laserpuls iväg och fluorescensen samlas in med hjälp av ett teleskop. Med denna metod kan alger liksom andra substanser på byggnadsfasader detekteras. Samma system användes för att mäta alghalten och organiska föroreningar i Medelhavet utanför Italiens västkust. Här riktades laserstrålen rakt ner i havet från ett undersökningsfartyg, och fluorescensen från olika djup samlades in. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/464989
- author
- Johansson, Thomas LU
- supervisor
- opponent
-
- Prof. van den Bergh, Hubert, Ecole Polytechnique Fédéral de Lausanne, Switzerland
- organization
- publishing date
- 2002
- type
- Thesis
- publication status
- published
- subject
- keywords
- fluorescence, spectroscopic techniques, fluorescence imaging, capillary electrophoresis, acoustic levitation, remote sensing, interstitial photodynamic therapy, multivariate analysis, Laserteknik, Laser technology, tissue optics, medical imaging, Fysicumarkivet A:2002:Johansson
- in
- Lund Reports on Atomic Physics
- pages
- 228 pages
- publisher
- Department of Physics, Lund University
- defense location
- Sölvegatan 14, Hall B
- defense date
- 2002-10-04 10:15:00
- external identifiers
-
- other:ISRN: LUTFD2(TFAF-1049)1-65(2002)
- ISSN
- 0281-2762
- ISBN
- ISBN 91-628-5369-4
- language
- English
- LU publication?
- yes
- additional info
- Article: S. Santesson, E. Cedergren, T. Johansson, T. Laurell, J. Nilsson and S. Nilsson, Screening of protein nucleation conditions using levitated drops, Submitted to Analytical Chemistry, July (2002). Article: S. Santesson, M. Andersson, E. Degerman, T. Johansson, J. Nilsson and S. Nilsson, Airborne cell analysis, Analalytical Chemistry 72, 3412 (2000). Article: T. Johansson, M. Petersson, J. Johansson and S. Nilsson, Real-time imaging through optical fiber array-assisted laser-induced fluorescence of capillary electrophoretic enantiomer separations, Analytical Chemistry 71, 4190 (1999). Article: J. Johansson, T. Johansson and S. Nilsson, Fluorescence imaging of light absorption for axial-beam geometry in capillary electrophoresis, Electrophoresis 19, 2233 (1998). Article: G. Cecchi, M. Bazzani, C. Cucci, D. Lognoli, I. Mochi, L. Pantani, V. Raimondi, R. Carlà, B. Breschi, D. Novelli, T. Johansson, P. Weibring, H. Edner and S. Svanberg, Probing the marine environment with fluorescence lidars - Evaluation of three fluorosensors in a field campaign, Manuscript. Article: P. Weibring, T. Johansson, H. Edner, S. Svanberg, B. Sundnér, V. Raimondi, G. Cecchi and L. Pantani, Fluorescence lidar imaging of historical monuments, Applied Optics 40, 6111 (2001). Article: D. Lognoli, G. Cecchi, I. Mochi, L. Pantani, V. Raimondi, R. Chiari, T. Johansson, P. Weibring, H. Edner and S. Svanberg, Fluorescence lidar imaging of the Parma cathedral and baptistery, Submitted to Applied Physics B, July (2002). Article: T. Johansson, G. Valentini, S. Andersson-Engels, G. Canti, A. Pifferi, P. Taroni, R. Cubeddu, K. Svanberg and S. Svanberg, Spectroscopic characterisation of non-melanoma skin lesions using multi-colour and lifetime fluorescence imaging, Manuscript. Article: M. Stenberg, M. Soto Thompson, T. Johansson, S. Pålsson, C. af Klinteberg, S. Andersson-Engels, U. Stenram, S. Svanberg and K. Svanberg, Interstitial photodynamic therapy. Diagnostic measurements and treatment in rat malignant experimental tumours, SPIE 4161, (2000) Eds. I. J. Bigio, G.J. Pupples, K. Svanberg and R.W. Steiner. Article: T. Johansson, M. Soto Thompson, M. Stenberg, C. af Klinteberg, S. Andersson-Engels, S. Svanberg and K. Svanberg, Feasibility study of a system for combined light dosimetry and interstitial photodynamic treatment of massive tumors, Applied Optics 41, 1462 (2002). Article: M. Soto-Thompson, T. Johansson, N. Bendsoe, A. Derjabo, J. Kapostins, S. Pålsson, U. Stenram, S. Andersson-Engels, J. Spigulis, S. Svanberg and K. Svanberg, Photodynamic therapy of basal cell carcinoma with multi-fibre contact light delivery, Manuscript.
- id
- f23b884d-dc53-41fe-8ec7-cbd9ec776d5b (old id 464989)
- date added to LUP
- 2016-04-01 16:47:42
- date last changed
- 2019-05-21 18:11:12
@phdthesis{f23b884d-dc53-41fe-8ec7-cbd9ec776d5b,
abstract = {{In this thesis spectroscopic techniques have been used in the fields of analytical chemistry, environmental and medical applications. In analytical chemistry, laser induced fluorescence was used to image the separation of substances during capillary electrophoresis. Here, an optical fibre imaging technique facilitated a higher sensitivity compared with a lens imaging technique. The motion of the substances was also monitored. In a similar experiment, laser induced fluorescence was used to image substances that do not exhibit fluorescence. Levitated drops have also been used as a wall-free cuvette during the study of fat-cell behaviour and protein precipitation, where laser induced fluorescence detection was used in the first part and light scattering detection in the second part.<br/><br>
<br/><br>
For environmental monitoring a lidar (light detection and ranging) system was used. Here, measurements have been performed at different monumental buildings to detect both biodeteriogen invasion and protective treatment. The system was also moved onto a ship for experiments in the Mediterranean Sea. Laser induced fluorescence was here used for monitoring of chlorophyll contents in the seawater together with distributed organic matter (DOM). With the use of depth resolved measurements, the water absorption was studied.<br/><br>
<br/><br>
In medical applications, laser induced fluorescence techniques have been used for spectral imaging of human normal and tumour tissue. For the treatment of the tumours, photodynamic therapy was employed. Three to six optical fibres, connected to a diode laser, were used and inserted in the tumour tissue. A sensitising agent was utilised to demarcate tumour tissue by fluorescence, and also to induce cell death.}},
author = {{Johansson, Thomas}},
isbn = {{ISBN 91-628-5369-4}},
issn = {{0281-2762}},
keywords = {{fluorescence; spectroscopic techniques; fluorescence imaging; capillary electrophoresis; acoustic levitation; remote sensing; interstitial photodynamic therapy; multivariate analysis; Laserteknik; Laser technology; tissue optics; medical imaging; Fysicumarkivet A:2002:Johansson}},
language = {{eng}},
publisher = {{Department of Physics, Lund University}},
school = {{Lund University}},
series = {{Lund Reports on Atomic Physics}},
title = {{Applications of laser spectroscopy to analytical chemistry, environmental monitoring and medicine}},
url = {{https://lup.lub.lu.se/search/files/4782732/2370312.pdf}},
year = {{2002}},
}