Direct neural conversion from human fibroblasts using self-regulating and nonintegrating viral vectors.
Lau, Shong LU ; Rylander, Daniella LU ; Jakobsson, Johan LU
and Parmar, Malin
LU
(2014)
In Cell Reports
9(5).
p.1673-1680
- Abstract
- Recent findings show that human fibroblasts can be directly programmed into functional neurons without passing via a proliferative stem cell intermediate. These findings open up the possibility of generating subtype-specific neurons of human origin for therapeutic use from fetal cell, from patients themselves, or from matched donors. In this study, we present an improved system for direct neural conversion of human fibroblasts. The neural reprogramming genes are regulated by the neuron-specific microRNA, miR-124, such that each cell turns off expression of the reprogramming genes once the cell has reached a stable neuronal fate. The regulated system can be combined with integrase-deficient vectors, providing a nonintegrative and... (More)
- Recent findings show that human fibroblasts can be directly programmed into functional neurons without passing via a proliferative stem cell intermediate. These findings open up the possibility of generating subtype-specific neurons of human origin for therapeutic use from fetal cell, from patients themselves, or from matched donors. In this study, we present an improved system for direct neural conversion of human fibroblasts. The neural reprogramming genes are regulated by the neuron-specific microRNA, miR-124, such that each cell turns off expression of the reprogramming genes once the cell has reached a stable neuronal fate. The regulated system can be combined with integrase-deficient vectors, providing a nonintegrative and self-regulated conversion system that rids problems associated with the integration of viral transgenes into the host genome. These modifications make the system suitable for clinical use and therefore represent a major step forward in the development of induced neurons for cell therapy. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4908805
- author
- Lau, Shong
LU
; Rylander, Daniella
LU
; Jakobsson, Johan
LU
and Parmar, Malin
LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Cell Reports
- volume
- 9
- issue
- 5
- pages
- 1673 - 1680
- publisher
- Cell Press
- external identifiers
-
- pmid:25482564
- wos:000346851900013
- scopus:84915749642
- pmid:25482564
- ISSN
- 2211-1247
- DOI
- 10.1016/j.celrep.2014.11.017
- language
- English
- LU publication?
- yes
- id
- 14515893-893a-45a8-a47f-a019df119196 (old id 4908805)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/25482564?dopt=Abstract
- date added to LUP
- 2016-04-01 13:40:01
- date last changed
- 2022-04-29 21:21:05
@article{14515893-893a-45a8-a47f-a019df119196,
abstract = {{Recent findings show that human fibroblasts can be directly programmed into functional neurons without passing via a proliferative stem cell intermediate. These findings open up the possibility of generating subtype-specific neurons of human origin for therapeutic use from fetal cell, from patients themselves, or from matched donors. In this study, we present an improved system for direct neural conversion of human fibroblasts. The neural reprogramming genes are regulated by the neuron-specific microRNA, miR-124, such that each cell turns off expression of the reprogramming genes once the cell has reached a stable neuronal fate. The regulated system can be combined with integrase-deficient vectors, providing a nonintegrative and self-regulated conversion system that rids problems associated with the integration of viral transgenes into the host genome. These modifications make the system suitable for clinical use and therefore represent a major step forward in the development of induced neurons for cell therapy.}},
author = {{Lau, Shong and Rylander, Daniella and Jakobsson, Johan and Parmar, Malin}},
issn = {{2211-1247}},
language = {{eng}},
number = {{5}},
pages = {{1673--1680}},
publisher = {{Cell Press}},
series = {{Cell Reports}},
title = {{Direct neural conversion from human fibroblasts using self-regulating and nonintegrating viral vectors.}},
url = {{https://lup.lub.lu.se/search/files/3514759/7697157.pdf}},
doi = {{10.1016/j.celrep.2014.11.017}},
volume = {{9}},
year = {{2014}},
}