Effect of cell-derived growth factors and cytokines on the clonal outgrowth of EBV-infected B cells and established lymphoblastoid cell lines
(1993) In Human Antibodies and Hybridomas 4(3). p.115-123- Abstract
Epstein-Barr virus (EBV) is a potent inducer of polyclonal B lymphocyte proliferation and is widely used as a tool for the establishment of B cell lines producing human monoclonal antibodies. However, because of low transformability, low clonability, and the inherent instability of EBV-infected B cells, valuable antibody-producing B cells are often lost during this procedure. We have here examined various cell-derived cytokines for their ability to enhance both the cellular outgrowth of newly infected B cells and the clonability of infected B cells and lymphoblastoid cell lines. Our results show that the murine thymoma cell line EL-4 is superior to peripheral blood mononuclear cells in both cellular outgrowth and cloning experiments,... (More)
Epstein-Barr virus (EBV) is a potent inducer of polyclonal B lymphocyte proliferation and is widely used as a tool for the establishment of B cell lines producing human monoclonal antibodies. However, because of low transformability, low clonability, and the inherent instability of EBV-infected B cells, valuable antibody-producing B cells are often lost during this procedure. We have here examined various cell-derived cytokines for their ability to enhance both the cellular outgrowth of newly infected B cells and the clonability of infected B cells and lymphoblastoid cell lines. Our results show that the murine thymoma cell line EL-4 is superior to peripheral blood mononuclear cells in both cellular outgrowth and cloning experiments, whereas monocyte-derived factors and monocyte cell lines were less capable than peripheral blood mononuclear cells in enhancing cellular outgrowth and cloning. Furthermore, the human T cell hybridoma cell line MP6 that secretes a B cell growth and differentiation factor, recently identified as an isoform of thioredoxin, is also capable of stimulating EBV-infected B cells and lymphoblastoid cell lines. Co-cultivation of EBV-infected B cells with MP6 cells significantly enhanced the cloning efficiency at the 1 cell/well level. The present results also suggest that one potential role of the MP6-derived thioredoxin could be the up regulation of IL-6 receptor expression in EBV-infected B cells.
(Less)
- author
- Ifversen, P. ; Xiu-Mei, Z. ; Ohlin, M. LU ; Zeuthen, J. and Borrebaeck, C. A K LU
- organization
- publishing date
- 1993
- type
- Contribution to journal
- publication status
- published
- keywords
- B cells, EBV infection, MP6, Thioredoxin
- in
- Human Antibodies and Hybridomas
- volume
- 4
- issue
- 3
- pages
- 9 pages
- publisher
- Butterworth Scientific Ltd.
- external identifiers
-
- scopus:0027295397
- pmid:8395232
- ISSN
- 0956-960X
- language
- English
- LU publication?
- yes
- id
- 4bfe395b-64d4-48f9-98a3-296382794f7a
- date added to LUP
- 2016-04-19 14:16:24
- date last changed
- 2024-01-03 23:53:34
@article{4bfe395b-64d4-48f9-98a3-296382794f7a, abstract = {{<p>Epstein-Barr virus (EBV) is a potent inducer of polyclonal B lymphocyte proliferation and is widely used as a tool for the establishment of B cell lines producing human monoclonal antibodies. However, because of low transformability, low clonability, and the inherent instability of EBV-infected B cells, valuable antibody-producing B cells are often lost during this procedure. We have here examined various cell-derived cytokines for their ability to enhance both the cellular outgrowth of newly infected B cells and the clonability of infected B cells and lymphoblastoid cell lines. Our results show that the murine thymoma cell line EL-4 is superior to peripheral blood mononuclear cells in both cellular outgrowth and cloning experiments, whereas monocyte-derived factors and monocyte cell lines were less capable than peripheral blood mononuclear cells in enhancing cellular outgrowth and cloning. Furthermore, the human T cell hybridoma cell line MP6 that secretes a B cell growth and differentiation factor, recently identified as an isoform of thioredoxin, is also capable of stimulating EBV-infected B cells and lymphoblastoid cell lines. Co-cultivation of EBV-infected B cells with MP6 cells significantly enhanced the cloning efficiency at the 1 cell/well level. The present results also suggest that one potential role of the MP6-derived thioredoxin could be the up regulation of IL-6 receptor expression in EBV-infected B cells.</p>}}, author = {{Ifversen, P. and Xiu-Mei, Z. and Ohlin, M. and Zeuthen, J. and Borrebaeck, C. A K}}, issn = {{0956-960X}}, keywords = {{B cells; EBV infection; MP6; Thioredoxin}}, language = {{eng}}, number = {{3}}, pages = {{115--123}}, publisher = {{Butterworth Scientific Ltd.}}, series = {{Human Antibodies and Hybridomas}}, title = {{Effect of cell-derived growth factors and cytokines on the clonal outgrowth of EBV-infected B cells and established lymphoblastoid cell lines}}, volume = {{4}}, year = {{1993}}, }