Comparison of species identification of endocarditis associated viridans streptococci using rnpB genotyping and 2 MALDI-TOF systems.
Isaksson, Jenny ; Rasmussen, Magnus LU ; Nilson, Bo LU
; Stadler, Liselott Svensson
; Kurland, Siri
; Olaison, Lars
; Ek, Elisabeth
and Herrmann, Björn
(2015)
In Diagnostic Microbiology and Infectious Disease
81(4).
p.240-245
- Abstract
- Streptococcus spp. are important causes of infective endocarditis but challenging in species identification. This study compared identification based on sequence determination of the rnpB gene with 2 systems of matrix-assisted laser desorption ionization-time of flight mass spectrometry, MALDI Biotyper (Bruker) and VITEK MS IVD (bioMérieux). Blood culture isolates of viridans streptococci from 63 patients with infective endocarditis were tested. The 3 methods showed full agreement for all 36 isolates identified in the Anginosus, Bovis, and Mutans groups or identified as Streptococcus cristatus, Streptococcus gordonii, or Streptococcus sanguinis. None of the methods could reliably identify the 23 isolates to the species level when... (More)
- Streptococcus spp. are important causes of infective endocarditis but challenging in species identification. This study compared identification based on sequence determination of the rnpB gene with 2 systems of matrix-assisted laser desorption ionization-time of flight mass spectrometry, MALDI Biotyper (Bruker) and VITEK MS IVD (bioMérieux). Blood culture isolates of viridans streptococci from 63 patients with infective endocarditis were tested. The 3 methods showed full agreement for all 36 isolates identified in the Anginosus, Bovis, and Mutans groups or identified as Streptococcus cristatus, Streptococcus gordonii, or Streptococcus sanguinis. None of the methods could reliably identify the 23 isolates to the species level when designated as Streptococcus mitis, Streptococcus oralis, or Streptococcus tigurinus. In 7 isolates classified to the Mitis group, the rnpB sequences deviated strikingly from all reference sequences, and additional analysis of sodA and groEL genes indicated the occurrence of yet unidentified Streptococcus spp. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/5039881
- author
- Isaksson, Jenny
; Rasmussen, Magnus
LU
; Nilson, Bo
LU
; Stadler, Liselott Svensson
; Kurland, Siri
; Olaison, Lars
; Ek, Elisabeth
and Herrmann, Björn
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Diagnostic Microbiology and Infectious Disease
- volume
- 81
- issue
- 4
- pages
- 240 - 245
- publisher
- Elsevier
- external identifiers
-
- pmid:25616316
- wos:000352117200003
- scopus:84925156374
- pmid:25616316
- ISSN
- 1879-0070
- DOI
- 10.1016/j.diagmicrobio.2014.12.007
- language
- English
- LU publication?
- yes
- id
- 9ff77c46-4d57-45a2-a726-fbb0c0a04286 (old id 5039881)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/25616316?dopt=Abstract
- date added to LUP
- 2016-04-01 11:08:59
- date last changed
- 2023-03-26 03:38:21
@article{9ff77c46-4d57-45a2-a726-fbb0c0a04286,
abstract = {{Streptococcus spp. are important causes of infective endocarditis but challenging in species identification. This study compared identification based on sequence determination of the rnpB gene with 2 systems of matrix-assisted laser desorption ionization-time of flight mass spectrometry, MALDI Biotyper (Bruker) and VITEK MS IVD (bioMérieux). Blood culture isolates of viridans streptococci from 63 patients with infective endocarditis were tested. The 3 methods showed full agreement for all 36 isolates identified in the Anginosus, Bovis, and Mutans groups or identified as Streptococcus cristatus, Streptococcus gordonii, or Streptococcus sanguinis. None of the methods could reliably identify the 23 isolates to the species level when designated as Streptococcus mitis, Streptococcus oralis, or Streptococcus tigurinus. In 7 isolates classified to the Mitis group, the rnpB sequences deviated strikingly from all reference sequences, and additional analysis of sodA and groEL genes indicated the occurrence of yet unidentified Streptococcus spp.}},
author = {{Isaksson, Jenny and Rasmussen, Magnus and Nilson, Bo and Stadler, Liselott Svensson and Kurland, Siri and Olaison, Lars and Ek, Elisabeth and Herrmann, Björn}},
issn = {{1879-0070}},
language = {{eng}},
number = {{4}},
pages = {{240--245}},
publisher = {{Elsevier}},
series = {{Diagnostic Microbiology and Infectious Disease}},
title = {{Comparison of species identification of endocarditis associated viridans streptococci using rnpB genotyping and 2 MALDI-TOF systems.}},
url = {{http://dx.doi.org/10.1016/j.diagmicrobio.2014.12.007}},
doi = {{10.1016/j.diagmicrobio.2014.12.007}},
volume = {{81}},
year = {{2015}},
}