Production of 3-hydroxypropionic acid from 3-hydroxypropionaldehyde by recombinant Escherichia coli co-expressing Lactobacillus reuteri propanediol utilization enzymes.
Sabet Azad, Ramin LU ; Sardari, Roya R R LU ; Linares-Pastén, Javier LU
and Hatti-Kaul, Rajni
LU
(2015)
In Bioresource Technology
180.
p.214-221
- Abstract
- 3-Hydroxypropionic acid (3-HP) is an important platform chemical for the biobased chemical industry. Lactobacillus reuteri produces 3-HP from glycerol via 3-hydroxypropionaldehyde (3-HPA) through a CoA-dependent propanediol utilization (Pdu) pathway. This study was performed to verify and evaluate the pathway comprising propionaldehyde dehydrogenase (PduP), phosphotransacylase (PduL), and propionate kinase (PduW) for formation of 3-HP from 3-HPA. The pathway was confirmed using recombinant Escherichia coli co-expressing PduP, PduL and PduW of L. reuteri DSM 20016 and mutants lacking expression of either enzyme. Growing and resting cells of the recombinant strain produced 3-HP with a yield of 0.3mol/mol and 1mol/mol, respectively, from... (More)
- 3-Hydroxypropionic acid (3-HP) is an important platform chemical for the biobased chemical industry. Lactobacillus reuteri produces 3-HP from glycerol via 3-hydroxypropionaldehyde (3-HPA) through a CoA-dependent propanediol utilization (Pdu) pathway. This study was performed to verify and evaluate the pathway comprising propionaldehyde dehydrogenase (PduP), phosphotransacylase (PduL), and propionate kinase (PduW) for formation of 3-HP from 3-HPA. The pathway was confirmed using recombinant Escherichia coli co-expressing PduP, PduL and PduW of L. reuteri DSM 20016 and mutants lacking expression of either enzyme. Growing and resting cells of the recombinant strain produced 3-HP with a yield of 0.3mol/mol and 1mol/mol, respectively, from 3-HPA. 3-HP was the sole product with resting cells, while growing cells produced 1,3-propanediol as co-product. 3-HP production from glycerol was achieved with a yield of 0.68mol/mol by feeding recombinant E. coli with 3-HPA produced by L. reuteri and recovered using bisulfite-functionalized resin. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/5039949
- author
- Sabet Azad, Ramin
LU
; Sardari, Roya R R
LU
; Linares-Pastén, Javier
LU
and Hatti-Kaul, Rajni
LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Bioresource Technology
- volume
- 180
- pages
- 214 - 221
- publisher
- Elsevier
- external identifiers
-
- pmid:25614245
- wos:000349715700029
- scopus:84921459204
- pmid:25614245
- ISSN
- 1873-2976
- DOI
- 10.1016/j.biortech.2014.12.109
- language
- English
- LU publication?
- yes
- id
- 82ef6cfc-5a14-4787-ad91-4623c3e25a5e (old id 5039949)
- date added to LUP
- 2016-04-01 09:53:13
- date last changed
- 2022-04-11 23:50:48
@article{82ef6cfc-5a14-4787-ad91-4623c3e25a5e,
abstract = {{3-Hydroxypropionic acid (3-HP) is an important platform chemical for the biobased chemical industry. Lactobacillus reuteri produces 3-HP from glycerol via 3-hydroxypropionaldehyde (3-HPA) through a CoA-dependent propanediol utilization (Pdu) pathway. This study was performed to verify and evaluate the pathway comprising propionaldehyde dehydrogenase (PduP), phosphotransacylase (PduL), and propionate kinase (PduW) for formation of 3-HP from 3-HPA. The pathway was confirmed using recombinant Escherichia coli co-expressing PduP, PduL and PduW of L. reuteri DSM 20016 and mutants lacking expression of either enzyme. Growing and resting cells of the recombinant strain produced 3-HP with a yield of 0.3mol/mol and 1mol/mol, respectively, from 3-HPA. 3-HP was the sole product with resting cells, while growing cells produced 1,3-propanediol as co-product. 3-HP production from glycerol was achieved with a yield of 0.68mol/mol by feeding recombinant E. coli with 3-HPA produced by L. reuteri and recovered using bisulfite-functionalized resin.}},
author = {{Sabet Azad, Ramin and Sardari, Roya R R and Linares-Pastén, Javier and Hatti-Kaul, Rajni}},
issn = {{1873-2976}},
language = {{eng}},
pages = {{214--221}},
publisher = {{Elsevier}},
series = {{Bioresource Technology}},
title = {{Production of 3-hydroxypropionic acid from 3-hydroxypropionaldehyde by recombinant Escherichia coli co-expressing Lactobacillus reuteri propanediol utilization enzymes.}},
url = {{http://dx.doi.org/10.1016/j.biortech.2014.12.109}},
doi = {{10.1016/j.biortech.2014.12.109}},
volume = {{180}},
year = {{2015}},
}