Efficient poly(3-hydroxypropionate) production from glycerol using Lactobacillus reuteri and recombinant Escherichia coli harboring L. reuteri propionaldehyde dehydrogenase and Chromobacterium sp. PHA synthase genes.
(2015) In Bioresource Technology 180. p.172-176- Abstract
- Poly(3-hydroxypropionate), P(3HP), is a polymer combining good biodegradability with favorable material properties. In the present study, a production system for P(3HP) was designed, comprising conversion of glycerol to 3-hydroxypropionaldehyde (3HPA) as equilibrium mixture with 3HPA-hydrate and -dimer in aqueous system (reuterin) using resting cells of native Lactobacillus reuteri in a first stage followed by transformation of the 3HPA to P(3HP) using recombinant Escherichia coli strain co-expressing highly active coenzyme A-acylating propionaldehyde dehydrogenase (PduP) from L. reuteri and polyhydroxyalkanoate synthase (PhaCcs) from Chromobacterium sp. P(3HP) content of up to 40% (w/w) cell dry weight was reached, and the yield with... (More)
- Poly(3-hydroxypropionate), P(3HP), is a polymer combining good biodegradability with favorable material properties. In the present study, a production system for P(3HP) was designed, comprising conversion of glycerol to 3-hydroxypropionaldehyde (3HPA) as equilibrium mixture with 3HPA-hydrate and -dimer in aqueous system (reuterin) using resting cells of native Lactobacillus reuteri in a first stage followed by transformation of the 3HPA to P(3HP) using recombinant Escherichia coli strain co-expressing highly active coenzyme A-acylating propionaldehyde dehydrogenase (PduP) from L. reuteri and polyhydroxyalkanoate synthase (PhaCcs) from Chromobacterium sp. P(3HP) content of up to 40% (w/w) cell dry weight was reached, and the yield with respect to the reuterin consumed by the cells was 78%. Short biotransformation period (4.5h), lack of additives or expensive cofactors, and use of a cheap medium for cultivation of the recombinant strain, provides a new efficient and potentially economical system for P(3HP) production. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/5040203
- author
- Linares-Pastén, Javier LU ; Sabet Azad, Ramin LU ; Pessina, Laura LU ; Sardari, Roya R R LU ; Ibrahim, Mohammad H A LU and Hatti-Kaul, Rajni LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Bioresource Technology
- volume
- 180
- pages
- 172 - 176
- publisher
- Elsevier
- external identifiers
-
- pmid:25600014
- wos:000349715700023
- scopus:84921261451
- pmid:25600014
- ISSN
- 1873-2976
- DOI
- 10.1016/j.biortech.2014.12.099
- language
- English
- LU publication?
- yes
- id
- da8e7d19-5b6c-4ec7-8b9f-6e674da6e156 (old id 5040203)
- date added to LUP
- 2016-04-01 10:11:30
- date last changed
- 2022-04-04 03:19:52
@article{da8e7d19-5b6c-4ec7-8b9f-6e674da6e156, abstract = {{Poly(3-hydroxypropionate), P(3HP), is a polymer combining good biodegradability with favorable material properties. In the present study, a production system for P(3HP) was designed, comprising conversion of glycerol to 3-hydroxypropionaldehyde (3HPA) as equilibrium mixture with 3HPA-hydrate and -dimer in aqueous system (reuterin) using resting cells of native Lactobacillus reuteri in a first stage followed by transformation of the 3HPA to P(3HP) using recombinant Escherichia coli strain co-expressing highly active coenzyme A-acylating propionaldehyde dehydrogenase (PduP) from L. reuteri and polyhydroxyalkanoate synthase (PhaCcs) from Chromobacterium sp. P(3HP) content of up to 40% (w/w) cell dry weight was reached, and the yield with respect to the reuterin consumed by the cells was 78%. Short biotransformation period (4.5h), lack of additives or expensive cofactors, and use of a cheap medium for cultivation of the recombinant strain, provides a new efficient and potentially economical system for P(3HP) production.}}, author = {{Linares-Pastén, Javier and Sabet Azad, Ramin and Pessina, Laura and Sardari, Roya R R and Ibrahim, Mohammad H A and Hatti-Kaul, Rajni}}, issn = {{1873-2976}}, language = {{eng}}, pages = {{172--176}}, publisher = {{Elsevier}}, series = {{Bioresource Technology}}, title = {{Efficient poly(3-hydroxypropionate) production from glycerol using Lactobacillus reuteri and recombinant Escherichia coli harboring L. reuteri propionaldehyde dehydrogenase and Chromobacterium sp. PHA synthase genes.}}, url = {{http://dx.doi.org/10.1016/j.biortech.2014.12.099}}, doi = {{10.1016/j.biortech.2014.12.099}}, volume = {{180}}, year = {{2015}}, }