EHD2 regulates adipocyte function and is enriched at cell surface-associated lipid droplets in primary human adipocytes
(2019) In Molecular Biology of the Cell 30(10). p.1147-1159- Abstract
Adipocytes play a central role in energy balance, and dysfunctional adipose tissue severely affects systemic energy homeostasis. The ATPase EH domain-containing 2 (EHD2) has previously been shown to regulate caveolae, plasma membrane-specific domains that are involved in lipid uptake and signal transduction. Here, we investigated the role of EHD2 in adipocyte function. We demonstrate that EHD2 protein expression is highly up-regulated at the onset of triglyceride accumulation during adipocyte differentiation. Small interfering RNA-mediated EHD2 silencing affected the differentiation process and impaired insulin sensitivity, lipid storage capacity, and lipolysis. Fluorescence imaging revealed localization of EHD2 to caveolae, close to... (More)
Adipocytes play a central role in energy balance, and dysfunctional adipose tissue severely affects systemic energy homeostasis. The ATPase EH domain-containing 2 (EHD2) has previously been shown to regulate caveolae, plasma membrane-specific domains that are involved in lipid uptake and signal transduction. Here, we investigated the role of EHD2 in adipocyte function. We demonstrate that EHD2 protein expression is highly up-regulated at the onset of triglyceride accumulation during adipocyte differentiation. Small interfering RNA-mediated EHD2 silencing affected the differentiation process and impaired insulin sensitivity, lipid storage capacity, and lipolysis. Fluorescence imaging revealed localization of EHD2 to caveolae, close to cell surface-associated lipid droplets in primary human adipocytes. These lipid droplets stained positive for glycerol transporter aquaporin 7 and phosphorylated perilipin-1 following adrenergic stimulation. Further, EHD2 overexpression in human adipocytes increased the lipolytic signaling and suppressed the activity of transcription factor PPARγ. Overall, these data suggest that EHD2 plays a key role for adipocyte function.
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- author
- Morén, Björn LU ; Hansson, Björn LU ; Negoita, Florentina LU ; Fryklund, Claes LU ; Lundmark, Richard ; Göransson, Olga LU and Stenkula, Karin G. LU
- organization
- publishing date
- 2019
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Molecular Biology of the Cell
- volume
- 30
- issue
- 10
- pages
- 13 pages
- publisher
- American Society for Cell Biology
- external identifiers
-
- pmid:30811273
- scopus:85065230221
- ISSN
- 1059-1524
- DOI
- 10.1091/mbc.E18-10-0680
- language
- English
- LU publication?
- yes
- id
- 521e6dbd-60a3-473e-953a-b4437462270f
- date added to LUP
- 2019-05-24 11:53:53
- date last changed
- 2024-09-03 20:55:44
@article{521e6dbd-60a3-473e-953a-b4437462270f, abstract = {{<p>Adipocytes play a central role in energy balance, and dysfunctional adipose tissue severely affects systemic energy homeostasis. The ATPase EH domain-containing 2 (EHD2) has previously been shown to regulate caveolae, plasma membrane-specific domains that are involved in lipid uptake and signal transduction. Here, we investigated the role of EHD2 in adipocyte function. We demonstrate that EHD2 protein expression is highly up-regulated at the onset of triglyceride accumulation during adipocyte differentiation. Small interfering RNA-mediated EHD2 silencing affected the differentiation process and impaired insulin sensitivity, lipid storage capacity, and lipolysis. Fluorescence imaging revealed localization of EHD2 to caveolae, close to cell surface-associated lipid droplets in primary human adipocytes. These lipid droplets stained positive for glycerol transporter aquaporin 7 and phosphorylated perilipin-1 following adrenergic stimulation. Further, EHD2 overexpression in human adipocytes increased the lipolytic signaling and suppressed the activity of transcription factor PPARγ. Overall, these data suggest that EHD2 plays a key role for adipocyte function.</p>}}, author = {{Morén, Björn and Hansson, Björn and Negoita, Florentina and Fryklund, Claes and Lundmark, Richard and Göransson, Olga and Stenkula, Karin G.}}, issn = {{1059-1524}}, language = {{eng}}, number = {{10}}, pages = {{1147--1159}}, publisher = {{American Society for Cell Biology}}, series = {{Molecular Biology of the Cell}}, title = {{EHD2 regulates adipocyte function and is enriched at cell surface-associated lipid droplets in primary human adipocytes}}, url = {{http://dx.doi.org/10.1091/mbc.E18-10-0680}}, doi = {{10.1091/mbc.E18-10-0680}}, volume = {{30}}, year = {{2019}}, }