Protocol for Proteome Analysis of Group A Streptococcus
(2020) In Methods in Molecular Biology 2136. p.135-144- Abstract
This chapter describes a protocol for the separation of different cell fractions from Streptococcus pyogenes and sample preparation for analysis by mass spectrometry. The presented approach can be used for the analysis of all subcellular proteomes from S. pyogenes and enables identification of drug or vaccine targets within a certain cellular fraction. Here, proteins integral to the plasma membrane are of particular interest for the development of new antimicrobial therapies.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/522da726-eb8a-423d-8e22-e1f68d5dc78c
- author
- Wilk, Laura LU
- organization
- publishing date
- 2020-05-20
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Bacterial proteome, Cell wall, Cellular fractionation, Gram-positive bacteria, Mass spectrometry, Membrane, secretome, Streptococcus pyogenes
- host publication
- Group A Streptococcus : Methods and Protocols - Methods and Protocols
- series title
- Methods in Molecular Biology
- editor
- Proft, Thomas and Loh, Jacelyn M.S.
- volume
- 2136
- pages
- 10 pages
- publisher
- Humana Press
- external identifiers
-
- pmid:32430817
- scopus:85085155994
- ISSN
- 1940-6029
- 1064-3745
- ISBN
- 978-1-0716-0467-0
- 978-1-0716-0466-3
- DOI
- 10.1007/978-1-0716-0467-0_9
- language
- English
- LU publication?
- yes
- id
- 522da726-eb8a-423d-8e22-e1f68d5dc78c
- date added to LUP
- 2020-06-26 12:01:01
- date last changed
- 2024-07-10 18:40:43
@inbook{522da726-eb8a-423d-8e22-e1f68d5dc78c, abstract = {{<p>This chapter describes a protocol for the separation of different cell fractions from Streptococcus pyogenes and sample preparation for analysis by mass spectrometry. The presented approach can be used for the analysis of all subcellular proteomes from S. pyogenes and enables identification of drug or vaccine targets within a certain cellular fraction. Here, proteins integral to the plasma membrane are of particular interest for the development of new antimicrobial therapies.</p>}}, author = {{Wilk, Laura}}, booktitle = {{Group A Streptococcus : Methods and Protocols}}, editor = {{Proft, Thomas and Loh, Jacelyn M.S.}}, isbn = {{978-1-0716-0467-0}}, issn = {{1940-6029}}, keywords = {{Bacterial proteome; Cell wall; Cellular fractionation; Gram-positive bacteria; Mass spectrometry; Membrane; secretome; Streptococcus pyogenes}}, language = {{eng}}, month = {{05}}, pages = {{135--144}}, publisher = {{Humana Press}}, series = {{Methods in Molecular Biology}}, title = {{Protocol for Proteome Analysis of Group A Streptococcus}}, url = {{http://dx.doi.org/10.1007/978-1-0716-0467-0_9}}, doi = {{10.1007/978-1-0716-0467-0_9}}, volume = {{2136}}, year = {{2020}}, }