Release of cytokines and proteases from human peripheral blood mononuclear and polymorphonuclear cells following phagocytosis and LPS stimulation
(1996) In Scandinavian Journal of Clinical and Laboratory Investigation 56(5). p.461-470- Abstract
Release and cellular contents of pro- and anti-inflammatory cytokines, neutrophilic elastase and secretory leukocyte proteinase inhibitor (SLPI) were measured with enzyme-linked immunosorbent assay in peripheral blood mono- and polymorphonuclear cells stimulated with preopsonized yeast cells or lipopolysaccharide. Tumour necrosis factor alpha (TNFα) was also measured with a bioassay. TNFα production and soluble TNFα receptor I (sTNF RI) were demonstrated in the environment of both cell populations. The bioassay indicated levels of TNFα far below those detected by ELISA. The overall secretion of cytokines and their inhibitors was found to favour an anti-inflammatory balance in the environment of the stimulated cells. The interleukin-1... (More)
Release and cellular contents of pro- and anti-inflammatory cytokines, neutrophilic elastase and secretory leukocyte proteinase inhibitor (SLPI) were measured with enzyme-linked immunosorbent assay in peripheral blood mono- and polymorphonuclear cells stimulated with preopsonized yeast cells or lipopolysaccharide. Tumour necrosis factor alpha (TNFα) was also measured with a bioassay. TNFα production and soluble TNFα receptor I (sTNF RI) were demonstrated in the environment of both cell populations. The bioassay indicated levels of TNFα far below those detected by ELISA. The overall secretion of cytokines and their inhibitors was found to favour an anti-inflammatory balance in the environment of the stimulated cells. The interleukin-1 receptor antagonist (IL1-ra), compared with interleukin-1β (IL-1β), dominated the secretions from both cell types with a 100- to 1000-fold excess respectively. Most of the translated IL-1β was not secreted but found associated with the cellular compartments. In contrast to lipopolysaccharide (LPS) stimulation, preopsonized yeast cells stimulated a massive release of elastase from neutrophil cells.
(Less)
- author
- Ohlsson, K. ; Linder, C. ; Lundberg, E. and Axelsson, L. LU
- organization
- publishing date
- 1996
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cytokines, Interleukin-1, Interleukin-1 receptor antagonist, Leukocyte proteases, Secretory leukocyte proteinase inhibitor, Soluble tumour necrosis factor receptor I, Tumour necrosis factor
- in
- Scandinavian Journal of Clinical and Laboratory Investigation
- volume
- 56
- issue
- 5
- pages
- 10 pages
- publisher
- Informa Healthcare
- external identifiers
-
- pmid:8869669
- scopus:0029816325
- ISSN
- 0036-5513
- language
- English
- LU publication?
- yes
- id
- 524a69e7-8302-4aa8-b6c8-d79ae97d3df8
- date added to LUP
- 2017-03-10 13:16:15
- date last changed
- 2024-02-29 11:07:00
@article{524a69e7-8302-4aa8-b6c8-d79ae97d3df8,
abstract = {{<p>Release and cellular contents of pro- and anti-inflammatory cytokines, neutrophilic elastase and secretory leukocyte proteinase inhibitor (SLPI) were measured with enzyme-linked immunosorbent assay in peripheral blood mono- and polymorphonuclear cells stimulated with preopsonized yeast cells or lipopolysaccharide. Tumour necrosis factor alpha (TNFα) was also measured with a bioassay. TNFα production and soluble TNFα receptor I (sTNF RI) were demonstrated in the environment of both cell populations. The bioassay indicated levels of TNFα far below those detected by ELISA. The overall secretion of cytokines and their inhibitors was found to favour an anti-inflammatory balance in the environment of the stimulated cells. The interleukin-1 receptor antagonist (IL1-ra), compared with interleukin-1β (IL-1β), dominated the secretions from both cell types with a 100- to 1000-fold excess respectively. Most of the translated IL-1β was not secreted but found associated with the cellular compartments. In contrast to lipopolysaccharide (LPS) stimulation, preopsonized yeast cells stimulated a massive release of elastase from neutrophil cells.</p>}},
author = {{Ohlsson, K. and Linder, C. and Lundberg, E. and Axelsson, L.}},
issn = {{0036-5513}},
keywords = {{Cytokines; Interleukin-1; Interleukin-1 receptor antagonist; Leukocyte proteases; Secretory leukocyte proteinase inhibitor; Soluble tumour necrosis factor receptor I; Tumour necrosis factor}},
language = {{eng}},
number = {{5}},
pages = {{461--470}},
publisher = {{Informa Healthcare}},
series = {{Scandinavian Journal of Clinical and Laboratory Investigation}},
title = {{Release of cytokines and proteases from human peripheral blood mononuclear and polymorphonuclear cells following phagocytosis and LPS stimulation}},
volume = {{56}},
year = {{1996}},
}