The carbon starvation response of the ectomycorrhizal fungus Paxillus involutus.
Ellström, Magnus LU ; Shah, Firoz LU ; Johansson, Tomas LU ; Ahrén, Dag LU
; Persson, Per
LU
and Tunlid, Anders
LU
(2015)
In FEMS Microbiology Ecology
91(4).
- Abstract
- The amounts of carbon allocated to the fungal partner in ectomycorrhizal associations can vary substantially depending on the plant growth and the soil nutrient conditions, and the fungus may frequently be confronted with limitations in carbon. We used chemical analysis and transcriptome profiling to examine the physiological response of the ectomycorrhizal fungus Paxillus involutus to carbon starvation during axenic cultivation. Carbon starvation induced a decrease in the biomass. Concomitantly, ammonium, cell-wall material (chitin) and proteolytic enzymes were released into the medium, which suggest autolysis. Compared with the transcriptome of actively growing hyphae, about 45% of the transcripts analyzed were differentially regulated... (More)
- The amounts of carbon allocated to the fungal partner in ectomycorrhizal associations can vary substantially depending on the plant growth and the soil nutrient conditions, and the fungus may frequently be confronted with limitations in carbon. We used chemical analysis and transcriptome profiling to examine the physiological response of the ectomycorrhizal fungus Paxillus involutus to carbon starvation during axenic cultivation. Carbon starvation induced a decrease in the biomass. Concomitantly, ammonium, cell-wall material (chitin) and proteolytic enzymes were released into the medium, which suggest autolysis. Compared with the transcriptome of actively growing hyphae, about 45% of the transcripts analyzed were differentially regulated during C-starvation. Induced during starvation were transcripts encoding extracellular enzymes such as peptidases, chitinases, and laccases. In parallel, transcripts of N-transporters were upregulated, which suggest that some of the released nitrogen compounds were re-assimilated by the mycelium. The observed changes suggest that the carbon-starvation response in P. involutus is associated with complex cellular changes that involves autolysis, recycling of intracellular compounds by autophagy and reabsorption of the extracellular released material. The study provides molecular markers that can be used to examine the role of autolysis for the turnover and survival of the ectomycorrhizal mycelium in soils. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/5258455
- author
- Ellström, Magnus
LU
; Shah, Firoz
LU
; Johansson, Tomas
LU
; Ahrén, Dag
LU
; Persson, Per
LU
and Tunlid, Anders
LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- FEMS Microbiology Ecology
- volume
- 91
- issue
- 4
- publisher
- Oxford University Press
- external identifiers
-
- pmid:25778509
- wos:000355327300010
- scopus:84954239015
- pmid:25778509
- ISSN
- 1574-6941
- DOI
- 10.1093/femsec/fiv027
- project
- MICCS - Molecular Interactions Controlling soil Carbon Sequestration
- language
- English
- LU publication?
- yes
- id
- ab2fff3e-c53d-47cd-9f90-c9ef3445cf2c (old id 5258455)
- date added to LUP
- 2016-04-01 10:33:21
- date last changed
- 2022-02-17 19:14:33
@article{ab2fff3e-c53d-47cd-9f90-c9ef3445cf2c,
abstract = {{The amounts of carbon allocated to the fungal partner in ectomycorrhizal associations can vary substantially depending on the plant growth and the soil nutrient conditions, and the fungus may frequently be confronted with limitations in carbon. We used chemical analysis and transcriptome profiling to examine the physiological response of the ectomycorrhizal fungus Paxillus involutus to carbon starvation during axenic cultivation. Carbon starvation induced a decrease in the biomass. Concomitantly, ammonium, cell-wall material (chitin) and proteolytic enzymes were released into the medium, which suggest autolysis. Compared with the transcriptome of actively growing hyphae, about 45% of the transcripts analyzed were differentially regulated during C-starvation. Induced during starvation were transcripts encoding extracellular enzymes such as peptidases, chitinases, and laccases. In parallel, transcripts of N-transporters were upregulated, which suggest that some of the released nitrogen compounds were re-assimilated by the mycelium. The observed changes suggest that the carbon-starvation response in P. involutus is associated with complex cellular changes that involves autolysis, recycling of intracellular compounds by autophagy and reabsorption of the extracellular released material. The study provides molecular markers that can be used to examine the role of autolysis for the turnover and survival of the ectomycorrhizal mycelium in soils.}},
author = {{Ellström, Magnus and Shah, Firoz and Johansson, Tomas and Ahrén, Dag and Persson, Per and Tunlid, Anders}},
issn = {{1574-6941}},
language = {{eng}},
number = {{4}},
publisher = {{Oxford University Press}},
series = {{FEMS Microbiology Ecology}},
title = {{The carbon starvation response of the ectomycorrhizal fungus Paxillus involutus.}},
url = {{http://dx.doi.org/10.1093/femsec/fiv027}},
doi = {{10.1093/femsec/fiv027}},
volume = {{91}},
year = {{2015}},
}