Characterizing nanoparticles in complex biological media and physiological fluids with depolarized dynamic light scattering
(2015) In Nanoscale 7(14). p.5991-5997- Abstract
- Light scattering is one of the few techniques available to adequately characterize suspended nanoparticles (NPs) in real time and in situ. However, when it comes to NPs in multicomponent and optically complex aqueous matrices - such as biological media and physiological fluids - light scattering suffers from lack of selectivity, as distinguishing the relevant optical signals from the irrelevant ones is very challenging. We meet this challenge by building on depolarized scattering: Unwanted signals from the matrix are completely suppressed. This approach yields information with an unprecedented signal-to-noise ratio in favour of the NPs and NP-biomolecule corona complexes, which in turn opens the frontier to scattering-based studies... (More)
- Light scattering is one of the few techniques available to adequately characterize suspended nanoparticles (NPs) in real time and in situ. However, when it comes to NPs in multicomponent and optically complex aqueous matrices - such as biological media and physiological fluids - light scattering suffers from lack of selectivity, as distinguishing the relevant optical signals from the irrelevant ones is very challenging. We meet this challenge by building on depolarized scattering: Unwanted signals from the matrix are completely suppressed. This approach yields information with an unprecedented signal-to-noise ratio in favour of the NPs and NP-biomolecule corona complexes, which in turn opens the frontier to scattering-based studies addressing the behaviour of NPs in complex physiological/biological fluids. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/5281642
- author
- Balog, S. ; Rodriguez-Lorenzo, L. ; Monnier, C. A. ; Obiols-Rabasa, Marc LU ; Rothen-Rutishauser, B. ; Schurtenberger, Peter LU and Petri-Fink, A.
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Nanoscale
- volume
- 7
- issue
- 14
- pages
- 7 pages
- publisher
- Royal Society of Chemistry
- external identifiers
-
- wos:000351934700011
- scopus:84961290408
- pmid:25631245
- ISSN
- 2040-3372
- DOI
- 10.1039/c4nr06538g
- language
- English
- LU publication?
- yes
- id
- 60cfcc1f-dd69-4e61-8bfd-d2cc52d0f165 (old id 5281642)
- date added to LUP
- 2016-04-01 10:44:12
- date last changed
- 2023-11-10 04:08:43
@article{60cfcc1f-dd69-4e61-8bfd-d2cc52d0f165, abstract = {{Light scattering is one of the few techniques available to adequately characterize suspended nanoparticles (NPs) in real time and in situ. However, when it comes to NPs in multicomponent and optically complex aqueous matrices - such as biological media and physiological fluids - light scattering suffers from lack of selectivity, as distinguishing the relevant optical signals from the irrelevant ones is very challenging. We meet this challenge by building on depolarized scattering: Unwanted signals from the matrix are completely suppressed. This approach yields information with an unprecedented signal-to-noise ratio in favour of the NPs and NP-biomolecule corona complexes, which in turn opens the frontier to scattering-based studies addressing the behaviour of NPs in complex physiological/biological fluids.}}, author = {{Balog, S. and Rodriguez-Lorenzo, L. and Monnier, C. A. and Obiols-Rabasa, Marc and Rothen-Rutishauser, B. and Schurtenberger, Peter and Petri-Fink, A.}}, issn = {{2040-3372}}, language = {{eng}}, number = {{14}}, pages = {{5991--5997}}, publisher = {{Royal Society of Chemistry}}, series = {{Nanoscale}}, title = {{Characterizing nanoparticles in complex biological media and physiological fluids with depolarized dynamic light scattering}}, url = {{http://dx.doi.org/10.1039/c4nr06538g}}, doi = {{10.1039/c4nr06538g}}, volume = {{7}}, year = {{2015}}, }