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Pre-PCR processing strategies

Rådström, Peter LU ; Lövenklev, Maria LU ; Wolffs, Petra LU ; Löfström, Charlotta LU and Knutsson, Rickard LU (2004)
Abstract
A review describes the sample prepn. for biochem. methods, enrichment methods, immunol. methods, and phys. methods. The pretreatment of a complex biol. sample is crucial, and for successful PCR the following requirements have to be fulfilled: complete lack or low concn. of PCR-inhibitory components in the sample and sufficient concn. of target DNA. The aim of the pre-PCR treatment is to convert a complex biol. sample contg. the target microorganisms into PCR-amplifiable samples.
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author
; ; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
host publication
PCR technology : current innovations
publisher
CRC Press
external identifiers
  • scopus:4344599509
ISBN
0-8493-1184-5
language
English
LU publication?
yes
id
081bb468-cd74-4805-b47e-96dd2890826c (old id 530653)
date added to LUP
2016-04-04 10:16:54
date last changed
2022-04-08 05:21:57
@inbook{081bb468-cd74-4805-b47e-96dd2890826c,
  abstract     = {{A review describes the sample prepn. for biochem. methods, enrichment methods, immunol. methods, and phys. methods. The pretreatment of a complex biol. sample is crucial, and for successful PCR the following requirements have to be fulfilled: complete lack or low concn. of PCR-inhibitory components in the sample and sufficient concn. of target DNA. The aim of the pre-PCR treatment is to convert a complex biol. sample contg. the target microorganisms into PCR-amplifiable samples.}},
  author       = {{Rådström, Peter and Lövenklev, Maria and Wolffs, Petra and Löfström, Charlotta and Knutsson, Rickard}},
  booktitle    = {{PCR technology : current innovations}},
  isbn         = {{0-8493-1184-5}},
  language     = {{eng}},
  publisher    = {{CRC Press}},
  title        = {{Pre-PCR processing strategies}},
  year         = {{2004}},
}