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Tissue microarray is inappropriate for analysis of BCL6 expression in diffuse large B-cell lymphoma.

Linderoth, Johan LU ; Ehinger, Mats LU ; Åkerman, Måns LU ; Cavallin-Ståhl, Eva LU ; Enblad, Gunilla ; Erlanson, Martin and Jerkeman, Mats LU (2007) In European Journal of Haematology 79(2). p.146-149
Abstract
Objective: In this study, our aim was to investigate how different immunohistochemical techniques may influence the result of BCL6 positivity and categorization in germinal center (GC) and non-GC derived diffuse large B-cell lymphoma (DLBCL), as it has been proposed that classification of DLBCL according to cell-of-origin by immunohistochemistry may be performed as a routine procedure in the diagnostic workup. However, a number of technical issues need to be solved before introducing this as a standard technique. Methods: Tumor specimens from 122 patients with de novo stage II-IV disease, adequately treated with anthracycline-containing chemotherapy regimens were collected. Immunohistochemical expression of BCL6, CD10, and MUM-1/IRF4 was... (More)
Objective: In this study, our aim was to investigate how different immunohistochemical techniques may influence the result of BCL6 positivity and categorization in germinal center (GC) and non-GC derived diffuse large B-cell lymphoma (DLBCL), as it has been proposed that classification of DLBCL according to cell-of-origin by immunohistochemistry may be performed as a routine procedure in the diagnostic workup. However, a number of technical issues need to be solved before introducing this as a standard technique. Methods: Tumor specimens from 122 patients with de novo stage II-IV disease, adequately treated with anthracycline-containing chemotherapy regimens were collected. Immunohistochemical expression of BCL6, CD10, and MUM-1/IRF4 was examined using a tissue microarray (TMA) technique. BCL6 and CD10 were also evaluated on whole tissue sections. Results: Due to profound tissue heterogeneity, BCL6 showed a wide range of positivity, with a high number of false negative results by TMA (25% positive), compared to 53% on whole tissue sections (WTS). CD10 was more homogeneously expressed, and TMA results corresponded better to WTS. Consequently, the results from categorization into GC and non-GC DLBCL differed considerably by use of the two methods, and resulted in very different outcome in terms of overall survival. Conclusion: Immunohistochemical GC-status determined on TMA is not reliable enough to be used for individual treatment decisions in DLBCL, mostly due to difficulties in interpreting BCL6 status. (Less)
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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
bCL6, microarray, tissue, diffuse large B-cell lymphoma, immunohistochemistry
in
European Journal of Haematology
volume
79
issue
2
pages
146 - 149
publisher
Wiley-Blackwell
external identifiers
  • wos:000248450800008
  • scopus:34447344536
ISSN
1600-0609
DOI
10.1111/j.1600-0609.2007.00892.x
language
English
LU publication?
yes
id
378af8a5-1e97-4c52-a6db-0190b4290156 (old id 540695)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17635238&dopt=Abstract
date added to LUP
2016-04-01 12:06:33
date last changed
2022-01-26 22:57:23
@article{378af8a5-1e97-4c52-a6db-0190b4290156,
  abstract     = {{Objective: In this study, our aim was to investigate how different immunohistochemical techniques may influence the result of BCL6 positivity and categorization in germinal center (GC) and non-GC derived diffuse large B-cell lymphoma (DLBCL), as it has been proposed that classification of DLBCL according to cell-of-origin by immunohistochemistry may be performed as a routine procedure in the diagnostic workup. However, a number of technical issues need to be solved before introducing this as a standard technique. Methods: Tumor specimens from 122 patients with de novo stage II-IV disease, adequately treated with anthracycline-containing chemotherapy regimens were collected. Immunohistochemical expression of BCL6, CD10, and MUM-1/IRF4 was examined using a tissue microarray (TMA) technique. BCL6 and CD10 were also evaluated on whole tissue sections. Results: Due to profound tissue heterogeneity, BCL6 showed a wide range of positivity, with a high number of false negative results by TMA (25% positive), compared to 53% on whole tissue sections (WTS). CD10 was more homogeneously expressed, and TMA results corresponded better to WTS. Consequently, the results from categorization into GC and non-GC DLBCL differed considerably by use of the two methods, and resulted in very different outcome in terms of overall survival. Conclusion: Immunohistochemical GC-status determined on TMA is not reliable enough to be used for individual treatment decisions in DLBCL, mostly due to difficulties in interpreting BCL6 status.}},
  author       = {{Linderoth, Johan and Ehinger, Mats and Åkerman, Måns and Cavallin-Ståhl, Eva and Enblad, Gunilla and Erlanson, Martin and Jerkeman, Mats}},
  issn         = {{1600-0609}},
  keywords     = {{bCL6; microarray; tissue; diffuse large B-cell lymphoma; immunohistochemistry}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{146--149}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{European Journal of Haematology}},
  title        = {{Tissue microarray is inappropriate for analysis of BCL6 expression in diffuse large B-cell lymphoma.}},
  url          = {{https://lup.lub.lu.se/search/files/2785168/626065.pdf}},
  doi          = {{10.1111/j.1600-0609.2007.00892.x}},
  volume       = {{79}},
  year         = {{2007}},
}