A novel serotonin-containing tuft cell subpopulation in mouse intestine
(2019) In Cell and Tissue Research 376(2). p.189-197- Abstract
In this study, a novel subset of doublecortin-like kinase 1 (DCLK1)-immunoreactive (IR) tuft cells that also contain serotonin (5-hydroxytryptamine, 5HT) is described, in terms of their number, regional distribution, possible synthesis or reuptake of 5HT and proximity to 5-HT-containing enterochromaffin (EC) cells. The small intestine from C57BL/6J mice was divided into five segments while the large intestine was kept undivided. Double immunostaining was used to estimate numbers and topographic distribution of 5HT-IR (DCLK1/5HT) tuft cells and their possible expression of tryptophan hydroxylase (TPH) and serotonin transporter (SERT). Also, possible contacts between tuft cells and 5HT-IR EC cells were studied. In the small intestine, up... (More)
In this study, a novel subset of doublecortin-like kinase 1 (DCLK1)-immunoreactive (IR) tuft cells that also contain serotonin (5-hydroxytryptamine, 5HT) is described, in terms of their number, regional distribution, possible synthesis or reuptake of 5HT and proximity to 5-HT-containing enterochromaffin (EC) cells. The small intestine from C57BL/6J mice was divided into five segments while the large intestine was kept undivided. Double immunostaining was used to estimate numbers and topographic distribution of 5HT-IR (DCLK1/5HT) tuft cells and their possible expression of tryptophan hydroxylase (TPH) and serotonin transporter (SERT). Also, possible contacts between tuft cells and 5HT-IR EC cells were studied. In the small intestine, up to 80% of all tuft cells were identified as DCLK1/5HT-IR; in the large intestine, such cells were rare. The highest number of DCLK1/5HT-IR cells was found in the upper small intestine. The numbers of DCLK1/5HT-IR cells gradually decreased distally. DCLK1-IR tuft cells were not found to contain TPH, the rate-limiting enzyme in 5HT synthesis. SERT, the selective transporter for 5HT reuptake, could not convincingly be demonstrated in tuft cells. In villi and crypts, 3% and 10%, respectively, of all DCLK1-IR cells were in close proximity to EC cells. EC cells in close proximity to DCLK1-IR cells were, in villi and crypts, 3 and 8%, respectively. We conclude that DCLK1/5HT-IR cells constitute a novel subset of tuft cells that may have unique roles in the GI tract.
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- author
- Cheng, Xiaowen LU ; Voss, Ulrikke LU and Ekblad, Eva LU
- organization
- publishing date
- 2019-01-21
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Enterochromaffin cell, Serotonin, Serotonin transporter, Tryptophan hydroxylase, Tuft cell
- in
- Cell and Tissue Research
- volume
- 376
- issue
- 2
- pages
- 189 - 197
- publisher
- Springer
- external identifiers
-
- pmid:30666535
- scopus:85060328579
- ISSN
- 0302-766X
- DOI
- 10.1007/s00441-018-02988-3
- language
- English
- LU publication?
- yes
- id
- 54224ebe-aede-47f4-95af-0cde07aa675a
- date added to LUP
- 2019-02-01 09:40:55
- date last changed
- 2024-08-06 08:55:56
@article{54224ebe-aede-47f4-95af-0cde07aa675a, abstract = {{<p>In this study, a novel subset of doublecortin-like kinase 1 (DCLK1)-immunoreactive (IR) tuft cells that also contain serotonin (5-hydroxytryptamine, 5HT) is described, in terms of their number, regional distribution, possible synthesis or reuptake of 5HT and proximity to 5-HT-containing enterochromaffin (EC) cells. The small intestine from C57BL/6J mice was divided into five segments while the large intestine was kept undivided. Double immunostaining was used to estimate numbers and topographic distribution of 5HT-IR (DCLK1/5HT) tuft cells and their possible expression of tryptophan hydroxylase (TPH) and serotonin transporter (SERT). Also, possible contacts between tuft cells and 5HT-IR EC cells were studied. In the small intestine, up to 80% of all tuft cells were identified as DCLK1/5HT-IR; in the large intestine, such cells were rare. The highest number of DCLK1/5HT-IR cells was found in the upper small intestine. The numbers of DCLK1/5HT-IR cells gradually decreased distally. DCLK1-IR tuft cells were not found to contain TPH, the rate-limiting enzyme in 5HT synthesis. SERT, the selective transporter for 5HT reuptake, could not convincingly be demonstrated in tuft cells. In villi and crypts, 3% and 10%, respectively, of all DCLK1-IR cells were in close proximity to EC cells. EC cells in close proximity to DCLK1-IR cells were, in villi and crypts, 3 and 8%, respectively. We conclude that DCLK1/5HT-IR cells constitute a novel subset of tuft cells that may have unique roles in the GI tract.</p>}}, author = {{Cheng, Xiaowen and Voss, Ulrikke and Ekblad, Eva}}, issn = {{0302-766X}}, keywords = {{Enterochromaffin cell; Serotonin; Serotonin transporter; Tryptophan hydroxylase; Tuft cell}}, language = {{eng}}, month = {{01}}, number = {{2}}, pages = {{189--197}}, publisher = {{Springer}}, series = {{Cell and Tissue Research}}, title = {{A novel serotonin-containing tuft cell subpopulation in mouse intestine}}, url = {{http://dx.doi.org/10.1007/s00441-018-02988-3}}, doi = {{10.1007/s00441-018-02988-3}}, volume = {{376}}, year = {{2019}}, }