Oxidative stress, telomere shortening, and DNA methylation in relation to low-to-moderate occupational exposure to welding fumes.
Li, Huiqi LU ; Hedmer, Maria LU
; Wojdacz, Tomasz K
LU
; Hossain, Bakhtiar
LU
; Lindh, Christian
LU
; Tinnerberg, Håkan
LU
; Albin, Maria
LU
and Broberg Palmgren, Karin
LU
(2015)
In Environmental and Molecular Mutagenesis
56(8).
p.684-693
- Abstract
- Evidence suggests that exposure to welding fumes is a risk factor for lung cancer. We examined relationships between low-to-moderate occupational exposure to particles from welding fumes and cancer-related biomarkers for oxidative stress, changes in telomere length, and alterations in DNA methylation. We enrolled 101 welders and 127 controls (all currently nonsmoking men) from southern Sweden. We performed personal sampling of respirable dust and measured 8-oxodG concentrations in urine using a simplified liquid chromatography tandem mass spectrometry method. Telomere length in peripheral blood was measured by quantitative polymerase chain reaction. Methylation status of 10 tumor suppressor genes was determined by methylation-sensitive... (More)
- Evidence suggests that exposure to welding fumes is a risk factor for lung cancer. We examined relationships between low-to-moderate occupational exposure to particles from welding fumes and cancer-related biomarkers for oxidative stress, changes in telomere length, and alterations in DNA methylation. We enrolled 101 welders and 127 controls (all currently nonsmoking men) from southern Sweden. We performed personal sampling of respirable dust and measured 8-oxodG concentrations in urine using a simplified liquid chromatography tandem mass spectrometry method. Telomere length in peripheral blood was measured by quantitative polymerase chain reaction. Methylation status of 10 tumor suppressor genes was determined by methylation-sensitive high-resolution melting analysis. All analyses were adjusted for age, body mass index, previous smoking, passive smoking, current residence, and wood burning stove/boiler at home. Welders were exposed to respirable dust at 1.2 mg/m(3) (standard deviation, 3.3 mg/m(3) ; range, 0.1-19.3), whereas control exposures did not exceed 0.1 mg/m(3) (P < 0.001). Welders and controls did not differ in 8-oxodG levels (β = 1.2, P = 0.17) or relative telomere length (β = -0.053, P = 0.083) in adjusted models. Welders showed higher probability of adenomatous polyposis coli (APC) methylation in the unadjusted model (odds ratio = 14, P = 0.014), but this was not significant in the fully adjusted model (P = 0.052). Every working year as a welder was associated with 0.0066 units shorter telomeres (95% confidence interval -0.013 to -0.00053, P = 0.033). Although there were no clear associations between concentrations of respirable dust and the biomarkers, there were modest signs of associations between oxidative stress, telomere alterations, DNA methylation, and occupational exposure to low-to-moderate levels of particles. Environ. Mol. Mutagen., 2015. © 2015 Wiley Periodicals, Inc. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/5442169
- author
- Li, Huiqi
LU
; Hedmer, Maria
LU
; Wojdacz, Tomasz K
LU
; Hossain, Bakhtiar
LU
; Lindh, Christian
LU
; Tinnerberg, Håkan
LU
; Albin, Maria
LU
and Broberg Palmgren, Karin
LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Environmental and Molecular Mutagenesis
- volume
- 56
- issue
- 8
- pages
- 684 - 693
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:26013103
- wos:000363209100005
- scopus:84944274721
- pmid:26013103
- ISSN
- 1098-2280
- DOI
- 10.1002/em.21958
- language
- English
- LU publication?
- yes
- id
- 5fc14e9b-102b-4f04-890d-c9b305ff3011 (old id 5442169)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/26013103?dopt=Abstract
- date added to LUP
- 2016-04-01 10:24:55
- date last changed
- 2022-04-27 21:51:53
@article{5fc14e9b-102b-4f04-890d-c9b305ff3011,
abstract = {{Evidence suggests that exposure to welding fumes is a risk factor for lung cancer. We examined relationships between low-to-moderate occupational exposure to particles from welding fumes and cancer-related biomarkers for oxidative stress, changes in telomere length, and alterations in DNA methylation. We enrolled 101 welders and 127 controls (all currently nonsmoking men) from southern Sweden. We performed personal sampling of respirable dust and measured 8-oxodG concentrations in urine using a simplified liquid chromatography tandem mass spectrometry method. Telomere length in peripheral blood was measured by quantitative polymerase chain reaction. Methylation status of 10 tumor suppressor genes was determined by methylation-sensitive high-resolution melting analysis. All analyses were adjusted for age, body mass index, previous smoking, passive smoking, current residence, and wood burning stove/boiler at home. Welders were exposed to respirable dust at 1.2 mg/m(3) (standard deviation, 3.3 mg/m(3) ; range, 0.1-19.3), whereas control exposures did not exceed 0.1 mg/m(3) (P < 0.001). Welders and controls did not differ in 8-oxodG levels (β = 1.2, P = 0.17) or relative telomere length (β = -0.053, P = 0.083) in adjusted models. Welders showed higher probability of adenomatous polyposis coli (APC) methylation in the unadjusted model (odds ratio = 14, P = 0.014), but this was not significant in the fully adjusted model (P = 0.052). Every working year as a welder was associated with 0.0066 units shorter telomeres (95% confidence interval -0.013 to -0.00053, P = 0.033). Although there were no clear associations between concentrations of respirable dust and the biomarkers, there were modest signs of associations between oxidative stress, telomere alterations, DNA methylation, and occupational exposure to low-to-moderate levels of particles. Environ. Mol. Mutagen., 2015. © 2015 Wiley Periodicals, Inc.}},
author = {{Li, Huiqi and Hedmer, Maria and Wojdacz, Tomasz K and Hossain, Bakhtiar and Lindh, Christian and Tinnerberg, Håkan and Albin, Maria and Broberg Palmgren, Karin}},
issn = {{1098-2280}},
language = {{eng}},
number = {{8}},
pages = {{684--693}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Environmental and Molecular Mutagenesis}},
title = {{Oxidative stress, telomere shortening, and DNA methylation in relation to low-to-moderate occupational exposure to welding fumes.}},
url = {{http://dx.doi.org/10.1002/em.21958}},
doi = {{10.1002/em.21958}},
volume = {{56}},
year = {{2015}},
}