Helicobacter pylori outer membrane vesicles protect the pathogen from reactive oxygen species of the respiratory burst
(2018) In Frontiers in Microbiology 9(SEP).- Abstract
Outer membrane vesicles (OMVs) play an important role in the persistence of Helicobacter pylori infection. Helicobacter OMVs carry a plethora of virulence factors, including catalase (KatA), an antioxidant enzyme that counteracts the host respiratory burst. We found KatA to be enriched and surface-associated in OMVs compared to bacterial cells. This conferred OMV-dependent KatA activity resulting in neutralization of H2O2 and NaClO, and rescue of surrounding bacteria from oxidative damage. The antioxidant activity of OMVs was abolished by deletion of KatA. In conclusion, enrichment of antioxidative KatA in OMVs is highly important for efficient immune evasion.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/55a66bb9-17ca-4e7a-b4cf-510f7e3c4f8c
- author
- Lekmeechai, Sujinna LU ; Su, Yu Ching LU ; Brant, Marta LU ; Alvarado-Kristensson, Maria LU ; Vallström, Anna ; Obi, Ikenna ; Arnqvist, Anna and Riesbeck, Kristian LU
- organization
- publishing date
- 2018-09-07
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- H. pylori, KatA, Outer membrane vesicles, Oxidative burst, Reactive oxygen species
- in
- Frontiers in Microbiology
- volume
- 9
- issue
- SEP
- article number
- 1837
- publisher
- Frontiers Media S. A.
- external identifiers
-
- pmid:30245670
- scopus:85053053080
- ISSN
- 1664-302X
- DOI
- 10.3389/fmicb.2018.01837
- language
- English
- LU publication?
- yes
- id
- 55a66bb9-17ca-4e7a-b4cf-510f7e3c4f8c
- date added to LUP
- 2018-10-12 07:47:47
- date last changed
- 2024-04-01 11:00:41
@article{55a66bb9-17ca-4e7a-b4cf-510f7e3c4f8c, abstract = {{<p>Outer membrane vesicles (OMVs) play an important role in the persistence of Helicobacter pylori infection. Helicobacter OMVs carry a plethora of virulence factors, including catalase (KatA), an antioxidant enzyme that counteracts the host respiratory burst. We found KatA to be enriched and surface-associated in OMVs compared to bacterial cells. This conferred OMV-dependent KatA activity resulting in neutralization of H<sub>2</sub>O<sub>2</sub> and NaClO, and rescue of surrounding bacteria from oxidative damage. The antioxidant activity of OMVs was abolished by deletion of KatA. In conclusion, enrichment of antioxidative KatA in OMVs is highly important for efficient immune evasion.</p>}}, author = {{Lekmeechai, Sujinna and Su, Yu Ching and Brant, Marta and Alvarado-Kristensson, Maria and Vallström, Anna and Obi, Ikenna and Arnqvist, Anna and Riesbeck, Kristian}}, issn = {{1664-302X}}, keywords = {{H. pylori; KatA; Outer membrane vesicles; Oxidative burst; Reactive oxygen species}}, language = {{eng}}, month = {{09}}, number = {{SEP}}, publisher = {{Frontiers Media S. A.}}, series = {{Frontiers in Microbiology}}, title = {{Helicobacter pylori outer membrane vesicles protect the pathogen from reactive oxygen species of the respiratory burst}}, url = {{http://dx.doi.org/10.3389/fmicb.2018.01837}}, doi = {{10.3389/fmicb.2018.01837}}, volume = {{9}}, year = {{2018}}, }